[1]原志庆),崔静),千新来),等. 大肠癌组织中p16、Rb及p27基因启动子区甲基化状态检测*[J].郑州大学学报(医学版),2011,(03):379.
 YUAN Zhiqing),CUI Jing),QIAN Xinlai),et al. Detection of methylation status of p16,Rb and p27 gene promoter region in colorectal carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(03):379.
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 大肠癌组织中p16、Rb及p27基因启动子区甲基化状态检测*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年03期
页码:
379
栏目:
论著
出版日期:
2011-05-15

文章信息/Info

Title:
 Detection of methylation status of p16,Rb and p27 gene promoter region in colorectal carcinoma tissue
作者:
 原志庆12)崔静2)千新来2)贺国洋2)郑丽丽1)#
1)郑州大学第一附属医院内分泌科 郑州 450052
2)新乡医学院病理学教研室 新乡 453003
Author(s):
 YUAN Zhiqing12)CUI Jing2)QIAN Xinlai2)HE Guoyang2)ZHENG Lili1)
1)Department of Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
2)Department of Pathology, Xinxiang Medical College,Xinxiang 453003
关键词:
大肠癌DNA甲基化p16基因Rb基因p27基因
分类号:
R735.3
文献标志码:
A
摘要:
 目的:探讨大肠癌组织中p16、Rb和p27基因启动子区甲基化状态的改变及意义。方法:应用甲基化特异性聚合酶链反应方法检测56例大肠癌及其相应癌旁正常上皮、42例腺瘤组织中p16、Rb和p27基因启动子区甲基化状态。结果:腺瘤、癌组织中p16和p27基因启动子区甲基化率均较正常组织增加(χ2=4.680、9.091和4.008、10.618,P<0.05);Rb基因启动子区甲基化率无明显变化(χ2=2.513和3.125,P>0.05),而且甲基化水平低。癌组织中p16和p27基因启动子区甲基化状态与其蛋白表达负关联(rP分别为0.894和0.920,P<0.001),而Rb基因启动子区甲基化状态与蛋白表达无关联(rP=0.026,P=0.661)。p16基因启动子区高甲基化状态与大肠癌的分化程度、浸润深度及淋巴结转移有关(χ2=10.757,6.229和5.707,P<0.05),而p27高甲基化状态仅与淋巴结转移有关(χ2=10.475,P<0.001)。结论:p16和p27基因启动子区高甲基化在大肠癌的发生、演进和转移中可能起重要作用。

参考文献/References:

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备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目0124170620
#通讯作者,女,1956年6月生,教授,博士,主任医师,研究方向:内分泌疾病的防治,E-mail:zhengli1012@126.com
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