[1]王立东) #,任景丽)),宋昕),等.食管癌变过程中肿瘤相关蛋白的表达*[J].郑州大学学报(医学版),2009,(01):13-16.
 WANG Lidong),REN Jingli),SONG Xin),et al.Proteins profile in esophageal multistage carcinogenesis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):13-16.
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食管癌变过程中肿瘤相关蛋白的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
13-16
栏目:
食管癌研究
出版日期:
2009-01-01

文章信息/Info

Title:
Proteins profile in esophageal multistage carcinogenesis
作者:
王立东1) #任景丽1)2)宋昕1)申秋1)郭涛1)杜娴娟1)李吉林3)
1)河南省食管癌重点开放实验室;郑州大学第一附属医院;郑州大学基础医学院郑州4500522)郑州大学第二附属医院病理科郑州4500143)林州市姚村食管癌医院病理科林州4565924)郑州大学第二附属医院消化内科郑州4500145)河南省人民医院消化内科郑州4500036)首都医科大学同仁医院消化内科北京1000177)林州市中心医院外科林州456592
Author(s):
WANG Lidong1)REN Jingli12)SONG Xin1)SHEN Qiu1)GUO Tao1)etc
关键词:
食管肿瘤鳞状细胞癌蛋白表达癌前病变河南
Keywords:
esophageal neoplasm squamous cell carcinomaproteins expressionprecancerous lesionHenan
分类号:
R735.1
摘要:
观察河南省食管癌高发区居民正常食管、癌前病变和癌组织中肿瘤相关蛋白表达的变化,探讨该地区居民食管癌变多阶段演进的分子基础。方法:采用免疫组织化学技术对来自河南省食管癌高发区居民的正常食管(1400例)、癌前病变(基底细胞过度增生1679例,不典型增生826例,原位癌380例)和癌组织(鳞状细胞癌2788例)中65种肿瘤相关蛋白进行检测,对阳性表达频率及其与病变进展关系进行生物信息学分析。结果:随病变程度加重,P53、Rb、RARβ、TGFβ1、Survivin、Bcl2、Cmyc、MDM2、Id1、NFkβp50、polβ、P40、EGFR、sPLA2ⅡA等14种蛋白表达升高,而P62、Koc、CyclinB1、IMP1、P21WAF1、XPC、MGMT、P16等8种蛋白表达下降。结论:上述蛋白有可能成为高危人群预警和早期发现的一组重要候选分子标志物。
Abstract:
To characterize the alterations of protein profiles in esophageal multistage carcinogenesis from normal to different precancerous and cancerous lesions in high incidence area for esophageal cancer (EC) in Henan and to elucidate the possible molecular mechanism of human esophageal multistage carcinogenesis.Methods:The 65 different proteins related with cancer suppressor gene and oncogenes were detected by immunohistochemistry in normal esophageal epithelia and in different esophageal precancerous and cancerous lesions. All the specimen were from the patients (n=7 073) at high incidence area for EC in Henan.Results: With the lesion progressed from normal (NOR) to basal cell hyperplasia (BCH),dysplasia (DYS),and esophageal squamous cell carcinoma (ESCC), the expressions of P53,Rb,RARβ,TGFβ1,Survivin,Bcl2,Cmyc,MDM2,Id1,NFkβp50,polβ,P40,EGFR and sPLA2ⅡA increased significantly; and the expressions of P62,koc,cyclinB1,IMP1,P21WAF1,XPC,MGMT and P16 showed declined tendency.Conclusion:The expression of the proteins identified in this study increases with the esophageal epithelial lesions progressed, which indicates that these proteins may be a group of promising molecular candidates for highrisk subject screening and early detection of EC.

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备注/Memo

备注/Memo:
#通讯作者,男,1958年生,博士,教授,研究方向:食管和贲门癌变机制和防治研究,Email:ldwang@zzu.edu.cn *美国NCI资助项目CA65871;国家自然科学基金资助项目30670956;国家科技部863重大资助项目2006AA02A403;973计划前期研究专项资助项目2007CB516812;河南省高校科技创新团队资助项目2008080411
更新日期/Last Update: 2010-05-18