[1]王立东)△,郭军辉),宋昕),等.食管贲门双源癌与单发食管癌和贲门癌基因组与蛋白质组对比*[J].郑州大学学报(医学版),2009,(01):24-27.
 WANG Lidong),GUO Junhui),SONG Xin),et al.Comparative genomic hybridization and proteomic analysis on esophageal and gastric cardia cancers from the same and different patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):24-27.
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食管贲门双源癌与单发食管癌和贲门癌基因组与蛋白质组对比*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
24-27
栏目:
食管癌研究
出版日期:
2009-01-01

文章信息/Info

Title:
Comparative genomic hybridization and proteomic analysis on esophageal and gastric cardia cancers from the same and different patients
作者:
王立东1)郭军辉1)宋昕1)冯笑山2)郭涛1)申秋1)袁翎1)3)等
1)河南省食管癌重点开放实验室;郑州大学第一附属医院;郑州大学基础医学院郑州4500522)河南科技大学第一附属医院肿瘤科洛阳4710033)河南省肿瘤医院放疗科郑州4500524)磁县医院病理科磁县0565005)林州市中心医院外科林州4565926)林州市姚村食管癌医院病理科林州456592
Author(s):
WANG Lidong1)GUO Junhui1)SONG Xin1)FENG Xiaoshan2)GUO Tao1)SHEN Qiu1)etc
关键词:
食管贲门双源癌蛋白质组比较基因组杂交表面增强激光解析离子化飞行时间质谱
Keywords:
concurrent esophageal and gastric cardia cancers proteomics CGH SELDITOFMS
分类号:
R735.1
摘要:
对食管贲门双源癌和单发食管癌及单发贲门癌患者血清蛋白质组和癌组织基因变化差异进行分析,筛选食管癌、贲门癌关键候选蛋白和基因。方法:采用表面增强激光解析离子化飞行时间质谱(SELDITOFMS)与比较基因组杂交(CGH)技术分析比较食管贲门双源癌(4例)和单发食管癌(107例)、单发贲门癌(86例)患者血清蛋白质组和癌组织全基因组。结果:根据SELDITOFMS蛋白质组所筛选的候选蛋白与CGH所筛选的基因进行一致性对比分析发现:双源癌中食管癌血清蛋白质组和癌组织CGH变异基因位点相一致的蛋白有14种(52%,11/21);双源癌中贲门癌与单发贲门癌位点相一致的蛋白有2种(Betadefensin123,GARP);双源癌、单发贲门癌和单发食管癌3者相一致的蛋白有1种(sPLA2ⅡA)。结论:所发现的基因变异位点与蛋白质组候选蛋白对比相一致的17种蛋白可能是食管和贲门癌变关键候选蛋白和基因。
Abstract:
To identify the key candidate genes and proteins related with esophageal and gastric cardia carcinogenesis by differential analysis on the patients with single esophageal or gastric cardia cancers or both from the same patient and proteomics.Methods:The surfaceenhanced laser description/ionization timeofflight mass spectrometry (SELDI TOFMS) and comparative genomic hybridization (CGH) were applied to the 4 patients with concurrent esophageal and gastric cardia cancers (CC),107 patsents with single esophageal cancer(EC) and 86 patients with gastric cardia cancer.Results: Based on the consistent analysis by CGH and SELDITOFMS, 14 cross proteins were identified both by CGH and SELDITOFMS on EC from CC and single EC patients (52%, 11/21); 2 cross proteins (Betadefens in 123, Gammaaminobutyric acid receptorassociated protein) on gastric cardia adenocarcinoma (GCA) from CC and single GCA patients, and 1 cross protein (Phospholipase A2, membrane associated) from the patients with CC, single EC and single GCA were identified.Conclusion: The seventeen cross proteins identified in this study may be a group of key candidates related with esophageal and gastric cardia carcinogenesis.

参考文献/References:

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备注/Memo

备注/Memo:
*国家科技部863重大资助项目2006AA02A403;国家自然科学基金资助项目30670956;河南省高校科技创新团队资助项目2008080411△男,1958年生,博士,教授,研究方向:食管和贲门癌变机制和防治研究,Email:ldwang@zzu.edu.cn
更新日期/Last Update: 2010-05-18