[1]丁广成)),王立东)#,任景丽),等.食管贲门双源癌癌组织中人乳头瘤状病毒和P16INK4A、P21WAF1蛋白检测*[J].郑州大学学报(医学版),2009,(01):31-33.
 DING Guangcheng),WANG Lidong),REN Jingli),et al.Detection of HPV16 infection and P16INK4A,P21WAF1 expression in concurrent esophageal and gastric cardia cancers from the same patient[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):31-33.
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食管贲门双源癌癌组织中人乳头瘤状病毒和P16INK4A、P21WAF1蛋白检测*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
31-33
栏目:
食管癌研究
出版日期:
2009-01-01

文章信息/Info

Title:
Detection of HPV16 infection and P16INK4A,P21WAF1 expression in concurrent esophageal and gastric cardia cancers from the same patient
作者:
丁广成1)2)王立东1)#任景丽13)李吉林4)郭军辉1)袁翎1)5)
1)河南省食管癌重点开放实验室;郑州大学第一附属医院;郑州大学基础医学院郑州4500522)郑州大学第五附属医院肿瘤放射治疗科郑州4500523)郑州大学第二附属医院病理科郑州4500144)林州市姚村食管癌医院病理科林州4565925)河南省肿瘤医院肿瘤放射治疗科郑州4500036)安阳市肿瘤医院胸外科安阳455000
Author(s):
DING Guangcheng12)WANG Lidong1)REN Jingli13) LI Jilin4)GUO Junhui1)etc
关键词:
食管肿瘤鳞状细胞癌贲门肿瘤腺癌人乳头状瘤病毒双源癌P16P21
Keywords:
esophageal neoplasmsquamous cell carcinoma gastric cardia neoplasmadenocarcinomahuman papillomavirusconcurrent cancers from esophagus and gastric cardia in same patientP16P21
分类号:
R735.1
摘要:
探讨食管贲门双源癌癌组织中人乳头瘤状病毒(HPV)感染、P16INK4A、P21WAF1蛋白表达与食管贲门癌变的关系。方法:采用PCR检测17例双源癌食管鳞癌(SCC)和贲门腺癌(GCA)组织HPV16型DNA(HPV16DNA),采用免疫组织化学方法检测P16INK4A、P21WAF1蛋白的表达。结果:17例SCC组织中HPV16DNA检出率为8/17,GCA组织为5/17,且有2例患者SCC和GCA组织中同时检测到HPV16DNA。2种组织中P16INK4A和P21WAF1蛋白均有不同程度表达,且表达的一致性较高(P均>0.05)。SCC、GCA组织中P16INK4A、P21WAF1蛋白表达与HPV16感染无关(P>0.05)。结论:HPV可能是SCC和GCA共同相关致病危险因素。
Abstract:
To detect the HPV16 infection,P16INK4A and P21WAF1 expression in concurrent esophageal and gastric cardia cancers(CC) from the same patient.Methods:HPV16DNA and expressions of P16INK4A and P21WAF1 were detected by PCR and ABC immunohistochemistry in 17 surgically resected specimens from CC patients.Results: The positive rate of HPV16DNA was 47%(8/17) in esophageal squamous cell carcinoma(SCC), and 29% (5/17) in gastric cardia adenocarcinoma(GCA) tissues, respectively, and HPV16DNA was simultaneously observed in SCC and GCA of 2 cases. The immunohistochemical staining for P16INK4A and P21WAF1 protein was observed both in SCC and GCA tissues with different degrees. The P16INK4A and P21WAF1 expression wasn't related to the incidence of HPV16 infection in SCC and GCA(P>0.05).Conclusion: HPV may be one of the common oncogenic factor for both SCC and GCA.

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备注/Memo

备注/Memo:
*河南省自然科学基金资助项目0511043200;河南省医学科技重大攻关项目WKJ20072028#通讯作者,男,1958年生,博士,教授,研究方向:食管和贲门癌变机制和防治研究,Email:ldwang@zzu.edu.cn
更新日期/Last Update: 2010-05-18