[1]贺付成),张岚),高冬玲),等.维生素D3对EC9706细胞增殖、裸鼠移植瘤生长及NDRG1蛋白表达的影响*[J].郑州大学学报(医学版),2009,(01):42-44.
 HE Fucheng),ZHANG Lan),GAO Dongling),et al.Effect of Vitamin D3 on EC9706 cells proliferation,xenograft tumor growth and expression of NDRG1 protein[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):42-44.
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维生素D3对EC9706细胞增殖、裸鼠移植瘤生长及NDRG1蛋白表达的影响* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
42-44
栏目:
食管癌研究
出版日期:
2009-01-01

文章信息/Info

Title:
Effect of Vitamin D3 on EC9706 cells proliferation,xenograft tumor growth and expression of NDRG1 protein
作者:
贺付成1)张岚2)高冬玲2)李惠翔2)陈奎生2)张云汉2)#
1)郑州大学第一附属医院检验科郑州4500522)河南省肿瘤病理重点实验室郑州450052
Author(s):
HE Fucheng1)ZHANG Lan2)GAO Dongling2)LI Huixiang2)CHEN Kuisheng2)ZHANG Yunhan2)
1)Department of Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Henan Key Laboraty of Tumor Pathology,Zhengzhou 450052
关键词:
125(OH)2维生素D3NDRG1EC9706细胞裸鼠
Keywords:
125(OH)2Vitamin D3Nmyc downstream regulated gene 1EC9706 cellsnude mice
分类号:
R735.1
摘要:
观察1,25(OH)2维生素D3对食管癌EC9706细胞细胞增殖、细胞周期、食管癌细胞裸鼠移植瘤生长及对NDRG1蛋白表达的影响。方法:采用0.3μmol/L的1,25(OH)2维生素D3作用于EC9706细胞后,MTT法检测肿瘤细胞的增殖情况,流式细胞仪检测细胞周期的变化。BALB/c小鼠荷瘤后,用5μg/kg1,25(OH)2维生素D3溶液皮下注射于移植瘤周围,观察移植瘤生长情况,并采用免疫组织化学方法检测裸鼠移植瘤组织中NDRG1蛋白表达水平。实验中均以未经1,25(OH)2维生素D3处理作为对照组。结果:①MTT实验结果显示,1,25(OH)2维生素D3作用1~5d时各组吸光度均低于对照组,差异有统计学意义(P<0.05);随着1,25(OH)2维生素D3作用时间的延长,细胞增殖抑制率逐渐升高(1d:7.4%,3d:21.1%,5d:23.8%)。②细胞周期检测结果显示,不同培养时间组G1期细胞分别与对照组比较,差异均有统计学意义(P<0.05)。③与对照组相比,1,25(OH)2维生素D3可上调NDRG1蛋白表达水平。结论:1,25(OH)2维生素D3可抑制食管癌细胞增殖,并通过上调NDRG1的表达而抑制食管癌裸鼠移植瘤的生长。
Abstract:
To study the proliferation,cell cycle distribution, expression of NDRG1 protein and xenograft tumor growth of human esophageal carcinoma EC9706 cells after being treated with 1,25(OH)2 Vitamin D3.Methods:EC9706 cells were treated with 0.3 μmol/L 1,25(OH)2 Vitamin D3,then cell proliferation was detected by MTT method and cell cycle distribution was observed by flow cytometer.The xenograft tumor was therapied by 5 μg/kg 1,25(OH)2 Vitamin D3 and the expression of NDRG1 protein in xenografts tumor was detected by immunohistochemistry method.Results:After EC9706 cells were exposed to 1,25(OH)2Vitamin D3 for 1~5 d, the mean absorbance values at 490 nm at different time points were lower than those in control group(P<0.05). The inhibition rate of proliferation at different time was:7.4%at day 1, 21.1% at day 3, 23.8% at day 5,respectively. The inhibition rate increased with time.Flow cytometry (FCM) revealed that after EC9706 cells being exposed to 1,25(OH)2 Vitamin D3 for 1~5 d,the cell cycle distribution changed significantly.There were significant differences in the percentages of the cells in G1 phase between experiment group and control group (P<0.05).1,25(OH)2 Vitamin D3 could upregulate the expression of NDRG1 protein.Conclusion: 1,25(OH)2 Vitamin D3 may inhibit growth of xenograft tumor through regulating expression of NDRG1.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30470779 #通讯作者,男,1938年生,教授,研究方向:肿瘤病理,Email:yhzhang@zzu.edu.cn
更新日期/Last Update: 2010-05-18