[1]吴欣爱)△,樊青霞),李鑫),等.乏氧对EC9706细胞多药耐药相关蛋白表达的影响[J].郑州大学学报(医学版),2009,(01):51-54.
 WU Xinai),FAN Qingxia),LI Xin),et al.Effect of hypoxia on expression of MRP1 and the mechanism in human EC9706 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):51-54.
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乏氧对EC9706细胞多药耐药相关蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
51-54
栏目:
食管癌研究
出版日期:
2009-01-01

文章信息/Info

Title:
Effect of hypoxia on expression of MRP1 and the mechanism in human EC9706 cell line
作者:
吴欣爱1)樊青霞1)李鑫1)王留兴1)王瑞林1)姜中兴2)#
1)郑州大学第一附属医院肿瘤科郑州4500522)郑州大学第一附属医院血液科郑州450052
Author(s):
WU Xin’ai1)FAN Qingxia1)LI Xin1)WANG Liuxing1)WANG Ruilin1)JIANG Zhongxing2)
1)Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Hematology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
乏氧诱导因子1α食管鳞状细胞癌多药耐药相关蛋白RNA干扰
Keywords:
hypoxiainducible factor1 αESCCmultidrug resistance proteinRNA interference
分类号:
R738.1
摘要:
研究乏氧对人食管鳞状细胞癌EC9706细胞株的多药耐药相关蛋白(MRP)表达的影响,探讨乏氧诱导因子1α(HIF1α)在乏氧引起肿瘤细胞多药耐药中的作用。方法:采用化学性低氧诱导剂氯化钴(CoCl2)体外培养人食管鳞状细胞癌EC9706细胞株,用RNAi沉默HIF1α基因表达,应用半定量RTPCR检测HIF1α基因沉默效果。应用RTPCR、免疫细胞化学技术检测RNA干扰前后乏氧条件下MRP1蛋白及RNA水平表达的变化。结果:乏氧后EC9706细胞中MRP1mRNA和蛋白的表达均增加(P<0.05)。针对HIF1α基因的siRNA干扰技术,能有效沉默HIF1α基因的表达。同样乏氧条件,转染HIF1αsiRNA后EC9706细胞较未转染及转染ControlsiRNA组细胞相比,MRP1mRNA和蛋白的表达均减少(P<0.05)。结论:HIF1α上调食管鳞状细胞癌细胞中MRP1的表达可能是乏氧条件下化疗耐药的机制之一;HIF1α可望成为新的食管鳞状细胞癌治疗的靶点。
Abstract:
To study the effect of hypoxia on expression of multidrug resistance protein(MRP1) and its mechanism of multidrug resistance in ESCC, and to provide a new target for treatment of ESCC.Methods:To establish a hypoxia model of EC9706 cells by a chemical hypoxia inducer (Cobalt chloride, CoCl2). RNAinterference targeting HIF1α was used to silence the expression of HIF1α in human EC9706 cell line. The effect of HIF1α gene silencing was measured by RTPCR. The expressions of MRP1 were measured respectively by RTPCR, immunocytochemistry in EC9706 cells under normia, hypoxia and after RNA interference under hypoxia.Results:HIF1α mRNA expression was obviously knocked down by small interferencing RNA(siRNA). Expression of MRP1 on mRNA and protein level was significantly increased in EC9706 cells after hypoxia(P<0.05). MRP1 expression on mRNA and protein level in cells transfected with HIF1α siRNA was significantly lower than those in untransfected group and transfected with control siRNA group under hypoxia(P<0.05).Conclusion: HIF1α can upregulate the expression of MRP1, which may involve in the chemotherapeutic resistance of EC9706 cells under hypoxia. HIF1α can be used as a new, tumorspecific target for anticancer therapy in ESCC.

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备注/Memo

备注/Memo:
△女,1966年生,博士,副教授,研究方向:恶性肿瘤基础与临床,Email:wuxinai66@yahoo.com.cn #通讯作者,男,1965年生,主任医师,研究方向:血液病基础与临床,Email:jzx8888@yahoo.com.cn
更新日期/Last Update: 2010-05-18