[1]刘桂芝),吴逸明)#.非小细胞肺癌组织中RASSF1A蛋白的表达*[J].郑州大学学报(医学版),2009,(01):90-92.
 LIU Guizhi),WU Yiming).RASSF1A protein expression in nonsmall cell lung cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):90-92.
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非小细胞肺癌组织中RASSF1A蛋白的表达* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
90-92
栏目:
论著
出版日期:
2009-01-01

文章信息/Info

Title:
RASSF1A protein expression in nonsmall cell lung cancer tissue
作者:
刘桂芝1)吴逸明2)#
1)郑州大学第一附属医院体检科郑州4500522)郑州大学公共卫生学院劳动卫生学与毒理学教研室郑州450001
Author(s):
LIU Guizhi1) WU Yiming2)
1)Department of Physical Examination,the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522) Department of Occupational Health and Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001
关键词:
肺肿瘤鳞状细胞癌腺癌RASSF1A
Keywords:
lung neoplasmsquamous celladenocarcinomaRASSF1A
分类号:
R734.2
摘要:
检测非小细胞肺癌(NSCLC)组织中RASSF1A蛋白的表达。方法:采用WesternBlot检测96例NSCLC患者癌组织及相应的癌旁正常肺组织中RASSF1A蛋白的表达。结果:①在NSCLC组织中,RASSF1A失表达或低表达率达60.4%(58/96),而相应的癌旁正常肺组织均正常表达,2者比较,差异有统计学意义(P<0.05)。②RASSF1A表达下调或缺失与肿瘤的病理分期和淋巴结转移有关(P<0.05),但与患者的性别、年龄、是否吸烟,肿瘤的组织学类型及分化程度无关(P>0.05)。结论:RASSF1A在中国人NSCLC组织中有较高比例的表达下调或缺失,该蛋白低表达或阴性表达可能促进NSCLC的发生、发展及淋巴结转移。
Abstract:
To observe the expression of RASSF1A protein in nonsmall cell lung cancer(NSCLC) tissue.Methods:Western Blot was used to detect the expression of RASSF1A protein in 96 cases of NSCLC tissue and matched normal lung tissue.Results: The RASSF1A protein expression was normal in all normal lung tissue far from cancer area but lower or loss in 60.4%(58/96) carcinoma tissue.The lower or loss expression of RASSF1A protein was related with pathological stage and lymph node metastasis (P<0.05),but not with gender,age,smoking,histological type or differentiation grade of tumors of the patients (all P>0.05).Conclusion: Lower or loss expression of RASSF1A protein is a frequent event in patients with NSCLS in China, which may play an important role in the malignant progress and lymph node metastasis of NSCLS.

参考文献/References:

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[2]Wistuba Ⅱ, Behrens C, Virmani AK, et al. High resolution chromosome 3p allelotyping of human lung cancer and preneoplastic/preinvasive bronchial epithelium reveals multiple, discontinuous sites of 3p allele loss and three regions of frequent breakpoints[J]. Cancer Res, 2000,60(7):1 949
[3]邵康, 肖智雄, 唐槐静, 等. RASSF1A转录本在肺癌组织中表达及其临床意义[J]. 中华医学杂志, 2003, 83(7): 569
[4]张森, 邵康, 张翠艳,等. 3p21.3区域肺癌相关基因RASSF1A的抑癌功能研究[J].中华医学杂志, 2005, 85(13): 908
[5]杨振华, 蔡映云, 孙丽华, 等. 非小细胞肺癌11个肿瘤相关基因启动子CpG岛甲基化的研究[J]. 临床内科杂志, 2005, 22(10): 708
[6]Marsit CJ, Kim DH, Liu M, et al. Hypermethylation of RASSF1A and BLU tumor suppressor genes in nonsmall cell lung cancer: implications for tobacco smoking during adolescence[J]. Int J Cancer, 2005, 114(2): 219

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30571552 #通讯作者,男,1944年生,教授,研究方向:职业性肺癌
更新日期/Last Update: 2010-05-18