[1]孙长宇)△,张贤强),杨观瑞).肝细胞癌组织中乙型肝炎病毒X基因、COX2及MMP9的表达[J].郑州大学学报(医学版),2009,(01):96-98.
 SUN Changyu),ZHANG Xianqiang),YANG Guanrui).Expression of HBX,COX2 and MMP9 in hepatocellular carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):96-98.
点击复制

肝细胞癌组织中乙型肝炎病毒X基因、COX2及MMP9的表达()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
96-98
栏目:
论著
出版日期:
2009-01-01

文章信息/Info

Title:
Expression of HBX,COX2 and MMP9 in hepatocellular carcinoma tissue
作者:
孙长宇1)张贤强1)杨观瑞2)
1)郑州大学第一附属医院感染科郑州4500522)河南省医学科学研究所郑州450052
Author(s):
SUN Changyu1)ZHANG Xianqiang1)YANG Guanrui2)
1)Department of Infection,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Henan Institute of Medical Sciences, Zhengzhou 450052
关键词:
肝细胞癌乙型肝炎病毒X基因环氧化酶2基质金属蛋白酶9
Keywords:
hepatocellularcarcinomahepatitis B virus X genecyclooxygenase2matrix metalloproteinase9
分类号:
R735.7
摘要:
检测人原发性肝细胞癌(HCC)组织中乙型肝炎病毒X基因(HBX)、环氧化酶2(COX2)与基质金属蛋白酶9(MMP9)蛋白的表达。方法:采用免疫组织化学方法检测49例HCC组织、癌旁组织及10例正常肝组织中HBX、COX2与MMP9蛋白的表达情况。结果:HCC组织中的HBX、COX2及MMP9蛋白的阳性表达率分别为69.4%、77.5%与61.2%,癌旁组织中的阳性表达率分别为57.1%、71.4%与79.6%,均明显高于正常肝组织(均为0.0%,P<0.05);HCC组织与癌旁组织中COX2及MMP9蛋白的表达与HBX蛋白的表达均分别呈线性相关;HCC组织中有转移与无转移组间HBX、COX2与MMP9蛋白的阳性表达率差异均有统计学意义(P<0.05)。结论:在HCC的发生发展与转移过程中HBX、COX2与MMP9蛋白具有重要作用。
Abstract:
To study the expression of MMP9, COX2 and HBX in primary hepatocellular carcinoma(HCC)tissue.Methods:The expression of MMP9, COX2 and HBX in 49 cases of HCC tissue and the adjacent nontumorous tissue,and 10 cases of normal liver tissue were detected by immunohistochemistry.Results: The positive rates of HBX, COX2 and MMP9 in HCC and the adjacent nontumorous tissue were 69.4%,77.5%,61.2% and 57.1%,71.4%, 79.6%,respectively, higher than those in normal liver tissue (P<0.05).There was significant lineal correlation among HBX,COX2 and MMP9 immunoreactivity in HCC tissue and adjacent nontumorous tissue. The expressions of HBX, COX2 and MMP9 in HCC with metastasis were significantly higher than those without metastasis (P<0.05).Conclusion: It suggests that HBX, COX2 and MMP9 may play important roles in the carcinogenesis, development and metastasis of HCC.

参考文献/References:

[1]Sirma H, Giannini C, Poussin K,et al. Hepatitis B virus X mutants, present in hepatocellular carcinoma tissue abrogate both the antiproliferative and transactivation effects of HBx[J]. Oncogene, 1999,18(34):4 848
[2]Soslow RA,Dannenberg AJ,Rush D,et al. COX2 is expressed in human pulmonary, colonic, and mammary tumors[J]. Cancer,2000,89(12):2 637
[3]Ristimaki A,Nieminen O,Saukkonen K,et al.Expression of cyclooxygenase2 in human transitional cell carcinoma of the urinary bladder[J]. Am J Pathol,2001,158(3):849
[4]Liabakk NB,Talbot I,Smith RA,et al.Matrix metalloprotease 2(MMP2) and matrix metalloprotease 9(MMP9)type IV collagenases in colorectal cancer[J].Cancer Res,1996,56(1):190
[5]Fromowitz FB, Viola MV, Chao S, et al.ras p2l expression in the progression of breast cancer[J].Hum Pathol,1987,18(12):1 268
[6]Elmore LW,Hancock AR,Chang SF, et al. Hepatitis B virus X protein and p53 tumor suppressor interactions in the modulation of apoptosis[J].Proc Natl Acad Sci U S A,1997,94(26):14 707
[7]Gottlob K, Fulco M, Levrero M, et al . The hapatitis B virus HBx protein inhibits capcase 3 activity[J].J Biol Chem,1998,273(50):33 347
[8]LaraPezzi E,Roche S,Andrisani OM,et al.The hepatitis B virus HBx protein induces adherens junction disruption in a srcdependent manner[J].Oncogene,2001,20(26):3 323
[9]Tsujii M,DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2[J]. Cell,1995,83(3):493
[10]Tsujii M,Kawano S,DuBois RN. Cyclooxygenase2 expression in human colon cancer cells increases metastatic potential[J]. Proc Natl Acad Sci U S A,1997,94(7):3 336
[11]Wu YL,Sun B,Zhang XJ, et al.Growth inhibition and apoptosis induction of Sulindac on Human gastric cancer cells[J]. World J Gastroenterol,2001,7(6):796
[12]Chan TA,Morin PJ,Vogelstein B,et al. Mechanisms underlying nonsteroidal antiinflammatory drugmediated apoptosis[J].Proc Natl Acad Sci U S A,1998,95(2):681
[13]Callejas NA,Casado M,Diaz Guerra MJ,et al.Expression of cyclooxygenase2 promotes the release of matrix metalloproteinase2 and 9 in fetal rat hepatocytes[J].Hepatology,2001,33(4):860
[14]Cao Y,Prescott SM.Many actions of cyclooxygenase2 in cellular dynamics and in cancer[J].J Cell Physiol,2002,190(3):279
[15]Hah N,Lee ST.An absolute role of PKCdependent NFkappaB activation for induction of MMP9 in hepatocellular carcinoma cells[J].Biochem Biophys Res Commun,2003,305(2):428
[16]Yamamoto H,Itoh F,Adachi Y, et al. Relation of enhanced secretion of active matrix metalloproteinases with tumor spread in human hepatocellular carcinom[J].Gastroenterology,1997,112(4):1 290
[17]Hayasaka A ,Suzuki N, Fujimoto N, et al. Elevated plasma levels of matalloproteinase9 (92kd type Ⅳ collagenase/geltinase B) in hepatocellular carcinom[J].Hepatology,1996,24(5):1 058
[18]Su F,Schneider RJ.Hepatitis B virus HBx protein activates transcription factor NFkappaB by acting on multiple cytoplasmic inhibitors of relrelated proteins[J].J Virol,1996,70(7):4 558
[19]Mestre JR, Mackrell PJ, Rivadeneira DE, et al.Redundancy in the signaling pathways and promoter elements regulating cyclooxygenase2 gene expression in endotoxintreated macrophage/monocytic cells[J].J Biol Chem,2001,276(6):3 977

相似文献/References:

[1]杨利霞)△,骈林萍),李超).肝细胞癌多普勒超声影像与微血管密度、血管内皮生长因子表达的相关性分析[J].郑州大学学报(医学版),2009,(05):1006.
 YANG Lixia),PIAN Linping),LI Chao).Relationship between tumoral vascularity with Doppler sonography,MVD and expression of VEGF in primary hepatocarcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1006.
[2]孙长宇),张贤强),江河清)#,等.肝细胞性肝癌组织中HBX蛋白与骨桥蛋白的表达[J].郑州大学学报(医学版),2009,(03):579.
 SUN Changyu),ZHANG Xianqiang),JIANG Heqing),et al.Expression of HBX and OPN in hepatocellular carcinoma and the clinical significances[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):579.
[3]王欣,张艳,王宁,等.小鼠腹水型肝癌多药耐药模型的建立[J].郑州大学学报(医学版),2010,(06):920.
[4]黄涛)△,宫东伟),周进学),等.肝细胞癌组织中三磷酸腺苷结合盒转运体G2和CD133蛋白的表达[J].郑州大学学报(医学版),2011,(01):31.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):31.
[5]李德宇△,陈孝平.肝细胞癌组织中血管生成素样蛋白3和MAPK蛋白的表达*[J].郑州大学学报(医学版),2011,(02):192.
 LI Deyu,CHEN Xiaoping.Expression of ANGPTL3 and MAPK protein in hepatocellular carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):192.
[6]张宝南,魏治鹏.血管内皮抑素联合肝动脉化疗栓塞对大鼠肝癌移植瘤组织血管生成的影响*[J].郑州大学学报(医学版),2011,(03):462.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):462.
[7]黄明宜)△,刘宾),何炜)#.黑色素瘤抗原1诱导特异性抗肝癌免疫应答*[J].郑州大学学报(医学版),2013,(03):376.
 HUANG Mingyi),LIU Bin),HE Wei).Cancer testis antigen MAGE1 can induce immune response for antitumor in vivo and in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(01):376.
[8]张香梅△,杨桂林,乐晓华,等.肝细胞癌合并乙型肝炎肝硬化患者癌组织中长链非编码RNA表达谱分析*[J].郑州大学学报(医学版),2015,(02):194.
 ZHANG Xiangmei,YANG Guilin,LE Xiaohua,et al.Expression profiles of long noncoding RNA in HCC patients suffering hepatitis B liver cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(01):194.
[9]文夏杰),鲁凤民),吕全军)#.术前凝血功能指标对HBV相关肝细胞癌术后生存状况的评估价值*[J].郑州大学学报(医学版),2017,(06):725.[doi:10.13705/j.issn.1671-6825.2017.06.015]
 WEN Xiajie),LU Fengmin),LYU Quanjun).Estimate value of blood coagulation function index before surgery for survival of HBV-related hepatocellular carcinoma patients after hepatectomy[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(01):725.[doi:10.13705/j.issn.1671-6825.2017.06.015]

备注/Memo

备注/Memo:
△男,1969年生,博士研究生,副教授,研究方向:乙肝相关性肝癌的防治,Email:scy691014@yahoo.com.cn
更新日期/Last Update: 2010-05-18