[1]樊剑鸣)△,张晓峰),张巧),等.重组苦瓜MAP30蛋白对大肠癌LoVo细胞凋亡的影响*[J].郑州大学学报(医学版),2009,(01):116-119.
 FAN Jianming),ZHANG Xiaofeng),ZHANG Qiao),et al.Cloning of Bitter Melon ribosome inactivating proteinMAP30 Gene and the study of apoptotic LoVo cells induced by recombinant MAP30[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):116-119.
点击复制

重组苦瓜MAP30蛋白对大肠癌LoVo细胞凋亡的影响*()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年01期
页码:
116-119
栏目:
论著
出版日期:
2009-01-01

文章信息/Info

Title:
Cloning of Bitter Melon ribosome inactivating proteinMAP30 Gene and the study of apoptotic LoVo cells induced by recombinant MAP30
作者:
樊剑鸣1)张晓峰2)张巧1)姚武1)徐玉宝1)
1)郑州大学公共卫生学院卫生毒理学教研室郑州4500012)郑州大学公共卫生学院营养与食品卫生学教研室郑州450001
Author(s):
FAN Jianming1)ZHANG Xiaofeng2)ZHANG Qiao1) YAO WU1)XU Yubao1)
1)Department of Toxicology,College of Public Health, Zhengzhou University, Zhengzhou 4500012)Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001
关键词:
苦瓜蛋白MAP30大肠肿瘤LoVo细胞细胞生长抑制率凋亡
Keywords:
MAP30LoVo cellsMTTapoptotsis
分类号:
R575.2
摘要:
克隆及重组苦瓜蛋白MAP30,观察MAP30对大肠癌LoVo细胞凋亡的影响。方法:RTPCR法克隆MAP30基因的cDNA序列,并表达、纯化MAP30蛋白;MTT法测定纯化的MAP30蛋白对LoVo细胞的生长抑制作用;彗星实验观察MAP30引起凋亡的LoVo细胞基因组的降解情况。结果:成功克隆了苦瓜蛋白MAP30基因;MAP30蛋白对LoVo细胞体外生长具有抑制作用;MAP30蛋白对LoVo细胞半数抑制质量浓度(IC50)为70.0mg/L。MAP30在体外能诱导LoVo细胞凋亡。结论:重组的苦瓜MAP30蛋白在体外能抑制LoVo细胞生长,并能诱导其凋亡。
Abstract:
To clone and study the inhibitory effect of recombinant MAP30 (momordica antiHIV protein of 30 ku) on LoVo in vitro.Methods:Evaluation of drug cytotoxicity to LoVo cells with MTT assay and the obvious apoptotic nuclei of the tumor cells were observed at the appropriate concentrations of MAP30 and the degradation of the genome of the apoptotic cells induced by MAP30 were also dectected by using the method of single cell gel electrophoresis (comet assay).Results: MAP30 possessed very high ability to inhibit the growth of LoVo. The antitumor activities (IC50) to LoVo cells of MAP30 was 70.0 mg/L,and can induce the apoptosis of LoVo cells.Conclusion: The study indicates that MAP30 can inhibit LoVo growth and induce the apoptosis of LoVo cells in vitro.

参考文献/References:

[1]Bourinbaiar AS,LeeHuang S.Potentiation of antiHIV activity of antiinflammatory drugs,dexamethasone and indomethacin,by MAP30,the antiviral agent from bitter melon[J].Biochem Biophys Res Commun,1995,208(2):779
[2]LeeHuang S, Huang PL,Chen HC,et al.AntiHIV and antitumor activities of recombinant MAP30 of bitter melon[J].Gene, 1995,161 (2):151
[3]Sikriwal D, Ghosh P, Batra JK. Ribosome inactivating protein saporin induces apoptosis through mitochondrial cascade, independent of translation inhibition[J]. Int J Biochem Cell Biol,2008,40(12):2 880
[4]Narayanan S, Surendranath K, Bora N, et al. Ribosome inactivating proteins and apoptosis[J]. FEBS Lett,2005,579(6):1 324
[5]罗福康,沈大斌,郑红.人趋化因子ELC的克隆与表达[J].第三军医大学学报,2005, 27(12):1 204
[6]庞建新,马仁强,刘兰梅,等.博落回总碱对肝癌细胞的毒性作用和体内抗肿瘤作用[J].第一军医大学学报, 2005,25(3):325
[7]Park SW, Prithiviraj B, Vepachedu R, et al. Isolation and purification of ribosomeinactivating proteins[J].Methods Mol Biol,2006,318:335

相似文献/References:

[1]樊剑鸣)△,马建新),高德富),等.苦瓜蛋白MAP30对大肠癌细胞株LoVo细胞生长及凋亡的影响*[J].郑州大学学报(医学版),2009,(05):983.
 FAN Jianming),MA Jianxin),GAO Defu),et al.Effects of MAP30 from bitter melon on cell growth and apoptosis of LoVo cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):983.

备注/Memo

备注/Memo:
*郑州大学博士引进项目
更新日期/Last Update: 2010-05-18