[1]李振伟),李月白),王义生)#.靶向PPARγRNA干扰对激素诱导家兔骨髓基质细胞成脂分化的影响*[J].郑州大学学报(医学版),2009,(02):272-274.
 LI Zhenwei),LI Yuebai),WANG Yisheng).Effects of RNAi for target on PPARγ to suppress adipogenic differentiation of MSCs of rabbit induced by steroid[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):272-274.
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靶向PPARγRNA干扰对激素诱导家兔骨髓基质细胞成脂分化的影响* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
272-274
栏目:
应用研究
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of RNAi for target on PPARγ to suppress adipogenic differentiation of MSCs of rabbit induced by steroid
作者:
李振伟1)李月白2)王义生1)#
1)郑州大学第一附属医院骨科;河南省高等学校临床医学重点学科开放实验室郑州4500522)郑州大学基础医学院生物化学教研室郑州450001
Author(s):
LI Zhenwei1)LI Yuebai2)WANG Yisheng1)
1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;Open Laboratory of Unode Science of Clinical Medicine of Henan Province, Zhengzhou 4500522)Department of Biochemistry, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
RNA干扰过氧化物酶体增殖子活化受体γ腺病毒载体激素骨髓基质细胞成脂分化
Keywords:
RNA interferenceperoxisome proliferatoractivated receptorγadenovirus vectorsteroidbone marrow stromal celladipogenic differentiationrabbit
分类号:
R681.8
摘要:
观察靶向过氧化物酶体增殖子活化受体γ(PPARγ)RNA干扰抑制激素诱导骨髓基质细胞成脂分化的作用。方法:选用家兔第2代骨髓基质细胞,随机分为6组:模型组(M组:给予10-7mol/L地塞米松),干扰1、2、3组(S1组、S2组、S3组:分别给予siRNA1、2、3腺病毒1×106U和10-7mol/L地塞米松),感染无关siRNA组(C组:给予siRNA4腺病毒1×106U和10-7mol/L地塞米松),对照组(N组:不给予siRNA腺病毒和地塞米松)。苏丹Ⅲ染色,光镜下进行脂肪细胞计数,并采用RTPCR方法检测细胞内PPARγmRNA的表达。结果:处理并培养细胞3d,S1组、S2组、S3组细胞内PPARγmRNA表达水平低于M组、C组(P<0.05),且与N组比较差异无统计学意义(P>0.05)。处理细胞14d后,S1组、S2组、S3组中分化为脂肪细胞的数量少于M组和C组(P<0.05),且与N组比较差异无统计学意义(P>0.05)。结论:siRNA能够有效阻断激素诱导的骨髓基质细胞中PPARγmRNA表达及其成脂分化。
Abstract:
To observe the effects of inhibition for the target RNAi to peroxisome proliferatoractivated receptorγ(PPARγ)on steroidinduced adipogenesis in marrow stromal cells(MSCs).Methods:2nd denesty MSCs,were selected and cultured. MSCs were randomly separated into 6 groups.Group M: the cells were treated with 10-7 mol/L dexamethasone. Group S1, S2, and S3: the cells were treated with siRNA1, siRNA2, and siRNA3 adenovirous 1×106 U corresbondingly and 10-7 mol/L dexamethasone. Group C: the cells were treated with siRNA4 adenovirous. Group N: the cells were treated without the adenovirous and dexamethasone. The cells were stained with Sudan Ⅲ, and then the number of adipocytes were counted on a linght microscope. The impression of PPARγ mRNA was detected by using RTPCR.Results: The impression of PPARγ mRNA in cells of group S1, S2, and S3 were significantly lower than those of group M and group C(P<0.05). The number of adipocytes were significantly lower in cells of group S1, S2, and S3 than those of group M and group C(P<0.05). There was no significant difference between group S1, S2, S3 and group N(P>0.05).Conclusion:siRNA could suppress the expression of PPARγ mRNA and inhibit steroidinduced adipogenesis in MSCs.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划基金资助项目2005016 #通讯作者,男,1951年生,硕士,教授,研究方向:骨坏死的发病机制与防治、关节脊柱外科,Email:wangyisheng@zzu.edu.cn
更新日期/Last Update: 2010-05-17