[1]朱宝菊),乔玉环)#,李印).canstatin基因治疗对小鼠人卵巢癌移植瘤生长及Caspase3、Flk1蛋白表达的影响*[J].郑州大学学报(医学版),2009,(02):292-294.
 ZHU Baoju),QIAO Yuhuan),LI Yin).Effects of canstatin gene theraphy on expression of Flk1 and Caspase3 and growth of human ovarian cancer in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):292-294.
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canstatin基因治疗对小鼠人卵巢癌移植瘤生长及Caspase3、Flk1蛋白表达的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
292-294
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of canstatin gene theraphy on expression of Flk1 and Caspase3 and growth of human ovarian cancer in mice
作者:
朱宝菊1)乔玉环1)#李印2)
1)郑州大学第一附属医院妇产科郑州4500522)河南省肿瘤医院胸外科郑州450008
Author(s):
ZHU Baoju1)QIAO Yuhuan1)LI Yin2)
1)Department of Gynaecology and Obstetrics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 4500522)Department of Thoracic Surgery,Tumor Hospital of Henan,Zhengzhou 450008
关键词:
基因治疗canstatinCaspase3Flk1卵巢癌小鼠
Keywords:
gene theraphy canstatin Caspase3 Flk1 ovarian cancermouse
分类号:
Q737.31
摘要:
探讨canstatin基因治疗对人卵巢癌移植瘤的影响。方法:18只裸鼠皮下接种人卵巢癌HO8910PM细胞制备人卵巢癌移植瘤模型,当移植瘤体积达到50mm3时,随机分3组治疗,分别注射腺病毒介导的canstatin基因(Adcan)、AdGFP和PBS,2次瘤内多点注射,间隔72h,每次4×1010pfu。治疗期间每周2次用游标卡尺测量皮下移植瘤的长径和短径并计算移植瘤体积;治疗30d后处死裸鼠,切取肿瘤检测肿瘤组织中Caspase3、Flk1蛋白的表达。结果:Adcan治疗组肿瘤体积小于其余2组(P<0.05)。3组肿瘤组织中均有坏死,Adcan治疗组坏死明显。Adcan治疗组Caspase3蛋白表达高于AdGFP组和PBS组(P<0.05),Flk1蛋白的表达低于其他2组(P<0.05)。结论:人canstatin基因可能通过诱导肿瘤细胞凋亡、抑制肿瘤的血管生成,从而抑制人卵巢癌细胞的生长。
Abstract:
To investigate the effects of canstatin gene theraphy on human ovarian cancer in BALB/C nude mice.Methods:Tumor xenografts were randomized into three groups, Adcan group,AdGFP group and PBS group,and were given recombinant adenoviruses Adcan,AdGFP and PBS injection into tumors,respectively,4×1010 pfu per injection and two injections separated by a 72hour interval. During the treatment period, the size of tumor was measured with caliper twice a week. All the animals were sacrificed in 30 days after injection, and tumors were resected for both HE and Flk1 and Caspase3 detection by immunohistochemical method.Results:The size of tumor in Adcan group was significantly smaller than those of PBS and AdGFP group(P<0.05). No obvious toxicity was observed in animals. The HE staining showed necrotic regions in tumors, especially in Adcan group. The expression of Flk1 in tumor of Adcan group were lower (P<0.05), however Caspase3 expression were higher compared with those of the two other groups(P<0.05) .Conclusion: Canstatin can inhibit the growth of ovarian cancer by restraining the angiogenesis of tumor and inducing apoptosis.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省卫生厅科技攻关基金资助项目200703064 #通讯作者,女,1946年生,大学本科,教授,研究方向:妇科肿瘤
更新日期/Last Update: 2010-05-17