[1]王亚莉)),许燕)),韩雪飞),等.人脐血单个核细胞移植对大鼠脊髓损伤的修复作用*[J].郑州大学学报(医学版),2009,(02):309-311.
 WANG Yali),XU Yan),HAN Xuefei),et al.Effects of transplantation of human cord blood mononuclear cells on function recovery after spinal cord injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):309-311.
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人脐血单个核细胞移植对大鼠脊髓损伤的修复作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
309-311
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of transplantation of human cord blood mononuclear cells on function recovery after spinal cord injury in rats
作者:
王亚莉1)2)许燕2)3)韩雪飞2)郭思3)鄢文海2)3)邢莹2)#
1)新乡医学院生理学与神经生物学教研室新乡4530002)郑州大学医学院干细胞研究中心郑州4500523)郑州大学基础医学院病理生理学教研室郑州450001
Author(s):
WANG Yali12)XU Yan23)HAN Xuefei2)GUO Si3)YAN Wenhai23)XING Ying2)
1)Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 4530002)Stem Cells Research Center,College of Medical Sciences,Zhengzhou University, Zhengzhou 4500523)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 450001
关键词:
脐血单个核细胞脊髓损伤分化迁移大鼠
Keywords:
cord blood mononuclear cell spinal cord injury differentiation immigration rat
分类号:
R744.1
摘要:
观察人脐血单个核细胞移植入大鼠脊髓损伤部位后的分化、迁移情况和损伤后功能的修复。方法:建立SD大鼠T10脊髓节段损伤模型,随机分为移植组和对照组,每组20只。分离人脐血单个核细胞,Hoechst33258标记后移植入脊髓损伤部位,观察迁移情况,并用免疫荧光双标法观察移植细胞是否可以表达神经元特异性烯醇化酶(NSE)和神经胶质原纤维酸性蛋白(GFAP),同时采用BBB运动功能评分观察脊髓功能的修复情况。结果:人脐血单个核细胞在移植区脊髓内存活,并有GFAP和NSE阳性表达的移植细胞,移植细胞沿脊髓长轴发生迁移,10周时最多可向脊髓头尾两侧迁移7mm。BBB评分结果显示,移植组4周后与对照组相比[(11.55±1.05)vs(5.93±0.80)]、10周后与对照组相比[(13.50±0.88)vs(8.07±0.82)],差异均有统计学意义(P<0.05)。结论:人脐血单个核细胞对脊髓损伤的功能修复有促进作用,可能是一种治疗脊髓损伤的新的种子细胞。
Abstract:
To observe the differentiation, immigration,and improvement of behavior after infusion human cord blood mononuclear cells (HCMNCs) in rat spinal cord injury model.Methods:The model of rat spinal cord injury at the level T10 was established and randomly divided into HCMNCs group and control group. HCMNCs labeled by Hoechst 33258 were infused to site of the injury to measure the immigration of HCMNCs. The differentiation of the transplanted cells was detected by the expression of neuron specific enolase (NSE) and glial fibrillary acidic protein(GFAP) by using immunofluorescence. Meanwhile, the recovery of function was assessed by the modified BBB score.Results: The transplanted HCMNCs could survive at least 10 weeks, with the expression of NSE and GFAP. The 7 mm immigration of HCMNCs from the site of injury to the terminals of the spinal cord after infusion 10 weeks was detected. Functional recovery determined by modified BBB score was significantly improved in the HCMNCs group compared with the control group both at 4 weeks(11.55±1.05,5.93±0.80,P<0.05) and 10 weeks (13.50±0.88,8.07±0.82,P<0.05) after infusion(P<0.05).Conclusion: HCMNCs could improve functional recovery of spinal cord injury and it may be a seed cell in the treatment of spinal cord injury.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目0424410085;河南省医学科技创新人才工程基金资助项目2004013 #通讯作者,男,1957年生,博士,教授,研究方向:神经损伤与功能重建,Email:xingy@zzu.edu.cn
更新日期/Last Update: 2010-05-17