[1]张静),杨波),常可亮),等.人脑胶质瘤组织中MAPK和磷酸化MAPK的表达*[J].郑州大学学报(医学版),2009,(02):315-316.
　ZHANG Jing),YANG Bo),CHANG Keliang),et al.Expression of MAPK and phosphorylated MAPK in human glioma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):315-316.
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人脑胶质瘤组织中MAPK和磷酸化MAPK的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
315-316
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Expression of MAPK and phosphorylated MAPK in human glioma tissue
作者:
张静1); 杨波2); 常可亮2); 李远2); 胡炜2); 王青峰2); 关方霞1)#
1)郑州大学生物工程系郑州4500012)郑州大学第一附属医院神经外科郑州450052
Author(s):
ZHANG Jing1); YANG Bo2); CHANG Keliang2); LI Yuan2); HU Wei2); WANG Qingfeng 2); GUAN Fangxia2)
1)Department of Bioengineering, Zhengzhou University, Zhengzhou 4500012)Department of Neurosurgery,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
脑胶质瘤; 丝裂原激活蛋白激酶
Keywords:
cerebral glioma; mitogenactivated protein kinase
分类号:
R739.41
摘要:
研究人脑胶质瘤组织中丝裂原激活蛋白激酶(MAPK)的表达及活化情况。方法：应用免疫组织化学技术观察新鲜冻存的16例脑胶质瘤组织标本(其中Ⅱ级9例，Ⅲ级7例)和5例脑外伤内减压术后正常脑组织中MAPK及磷酸化MAPK(pMAPK)的表达情况。结果：MAPK阳性表达位于胞质；pMAPK位于胞质和胞核，且胞核阳性信号较强。胶质瘤Ⅱ级、Ⅲ级和正常脑组织中MAPK阳性区域面积百分率分别为(37.78±4.05)%、(53.58±1.60)％和(23.12±2.43)%，pMAPK阳性区域面积百分率分别为(36.91±3.85)%、(53.14±3.47)%和(18.61±3.30)%。胶质瘤组织中MAPK及pMAPK阳性区域面积百分率均高于正常脑组织(P<0.05)；胶质瘤Ⅲ级者高于Ⅱ级者(P<0.05)。结论：MAPK及其异常活化与人脑胶质瘤的发生发展有关。
Abstract:
To explore the expression and abnormal activation of mitogenactivated protein kinase (MAPK) in the development and progression of cerebral gliomas.Methods:Immunohistochemistry was applied to detect expression of MAPK and phosphorylated MAPK(pMAPK） in 16 cases of gliomas and 5 cases of normal control cerebral tissues acquired from decompression surgery after traumatic brain injury.Results: MAPK expressed in cytoplasm, while the expression of pMAPK located at cell nucleus and cytoplasm, especially in nucleus. In grade Ⅱ, grade Ⅲ gliomas and normal brain tissues, the percentage of MAPK expression was (37.78±4.05)%, (53.58±1.60)％ and (23.12±2.43)% respectively; the expression of pMAPK was (36.91±3.85)%, (53.14±3.47)% and (18.61±3.30）% respectively. Expression of MAPK and pMAPK in gliomas was significantly higher than that in control tissues(P＜0.05). Grade Ⅲ gliomas had higher expression than gradeⅡsamples(P＜0.05).Conclusion: Abnormal expression of MAPK and pMAPK indict the altered cell biological behavior and correlated to pathological grades of gliomas.

参考文献/References:

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备注/Memo

备注/Memo:
*教育部新世纪优秀人才基金资助项目2006NCET060610；河南省高校青年骨干教师基金资助项目 #通讯作者，女，1970年生，教授，研究方向：肿瘤分子标记与防治，Email:guanfangxia@126.com
更新日期/Last Update: 2010-05-17