[1]袁峰,董铁立#,李长生.异丙酚鞘内注射对蜜蜂毒致痛大鼠脊髓组织中Fos蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):352-355.
 YUAN feng,DONG Tieli,LI Changsheng.Effects of intrathecal propofol on Fos protein expression in spinal cord of rats with bee venom induced pesistent pain[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):352-355.
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异丙酚鞘内注射对蜜蜂毒致痛大鼠脊髓组织中Fos蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
352-355
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of intrathecal propofol on Fos protein expression in spinal cord of rats with bee venom induced pesistent pain
作者:
袁峰董铁立#李长生
郑州大学第二附属医院麻醉科郑州450014
Author(s):
YUAN feng DONG Tieli LI Changsheng
Department of Anesthesiology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014
关键词:
异丙酚脊髓蜜蜂毒镇痛Fos大鼠
Keywords:
propofolspinal cordbee venomanalgesicFosrat
分类号:
R971
摘要:
通过对蜜蜂毒(BV)致痛大鼠蛛网膜下腔给予异丙酚,观察大鼠脊髓背角组织中Fos蛋白表达的改变,从组织形态学角度探讨异丙酚在脊髓水平的镇痛作用及其机制。方法:选取鞘内置管成功的60只SD大鼠随机分为致痛前给药组和致痛后给药组两大组。其中致痛前给药组包括:二甲基亚砜(DMSO)+生理盐水组(DN组)、DMSO+BV组(DB组)、异丙酚5μg+BV组(P5B组)、异丙酚10μg+BV组(P10B组)、异丙酚20μg+BV组(P20B组);致痛后给药组包括:生理盐水+DMSO组(ND组)、BV+DMSO组(BD组)、BV+异丙酚5μg组(BP5组)、BV+异丙酚10μg组(BP10组)、BV+异丙酚20μg组(BP20组)。每组均6只大鼠。DN组、DB组、P5B组、P10B组、P20B组在实验开始时分别鞘内注射DMSO5μL、DMSO5μL、异丙酚5μg、异丙酚10μg、异丙酚20μg,实验开始后15min除DN组在大鼠右侧后足底注射生理盐水50μL外,其余各组均注射体积分数0.4%BV50μL;ND组、BD组、BP5组、BP10组、BP20组在实验开始时除ND组在大鼠右侧后足底注射生理盐水50μL外,其余各组均注射体积分数0.4%BV50μL,实验开始后5min分别鞘内注射DMSO5μL、DMSO5μL、异丙酚5μg、异丙酚10μg、异丙酚20μg。所有药物单次注射容积均为5μL。各组大鼠足底注射后2h取脊髓L4~L6节段行Fos免疫组织化学染色,测定积分光密度(IOD)值及Fos蛋白表达抑制率。结果:BV致痛前,鞘内注射异丙酚(5、10和20μg)后,P5B、P10B、P20B组与DB、DN组比较,Fos表达的IOD值升高(P均<0.01);BV致痛后,鞘内注射同剂量异丙酚,BP5、BP10、BP20组与DN、BD组相比较,Fos表达的IOD值升高(P均<0.01)。且对大鼠脊髓背角Fos的表达产生剂量依赖性抑制作用(P<0.01),而且致痛后给药优于致痛前给药(P<0.05)。结论:异丙酚在脊髓水平具有镇痛作用且有剂量依赖性,对疼痛的治疗作用优于预防作用。
Abstract:
To assess the effects of intrathecal propofol on spinal cord Fos expression in rats following bee venom(BV) induced persistent pain,and to investigate the possible mechanism of analgesic of propofol in spinal level of rats from the view of histochemistry.Methods:A total of sixty male SD rats in which intrathecal (IT) catheters were successfully placed at L5~6 interspace were randomly divided into propain groups and pocpain groups. The propain groups were randomly divided into 5 groups:group Ⅰ,dimethyl sulphoxide(DMSO)+normal saline(DN); group Ⅱ,DMSO+BV(DB); group Ⅲ,propofol 5 μg+(P5B); group Ⅳ,propofol 10 μg+BV(P10B); group Ⅴ,propofol 20 μg+BV(P20B). The pocpain groups were randomly divided into 5 groups: group Ⅵ,normal saline+ DMSO(ND); group Ⅶ,BV+DMSO(BD); group Ⅷ,BV+propofol 5 μg(BP5); group Ⅸ,BV+propofol 10 μg(BP10); group Ⅹ,BV+propofol 20 μg (BP20).There were 6 rats in each group. Group DN, group DB, group P5B, group P10B,and group P20B were treated respectively with DMSO 5 μL IT, DMSO 5 μL IT,propofol 5 μg IT,propofol 10 μg IT,propofol 20 μg IT at the beginning of the experiment. After 15 min, except group DN was injected with normal saline 50 μL subcutaneously into the middle of plantar surface of right hindpaw, group DB, group P5B, group P10B,and group P20B were all injected with 0.4% BV 50 μL. Except group ND was injected with normal saline 50 μL subcutaneously into the middle of plantar surface of right hindpaw, group BD, group BP5, group BP10, group BP20 were all injected with 0.4% BV 50 μL at the beginning of the experiment. 5 min later, group ND, group BD, group BP5, group BP10, group BP20 were treated respectively with DMSO 5 μL IT, DMSO 5 μL IT,propofol 5 μg IT,propofol 10 μg IT,propofol 20 μg IT. The volume of single administration was 5 μL. After 2 hours, all the rats were deeply anesthetized and perfused intracardially; and then, the spinal cord was removed in the L4~L6 segments; finaly, the segments were examined for Fos expression by using Fos immunohistochemistry technique.Results: Before BVinduced pain, intrathecal injection of propofol (5, 10, and 20 μg),the value of IOD of the Fos expression was significantly increased when P5B, P10B, P20B group compared with DN and DB group(P<0.01). After BVinduced pain, intrathecal with the same dose of propofol, the value of IOD of the Fos expression was significantly increased when BP5, BP10, BP20 group compared with the DN and BD group (P<0.01). And Fos expression in the spinal dorsal horn also had a dosedependent inhibition(P<0.01). Propofol given IT pocpain produced dosedependent analgesia which was stronger than that propain produced(P<0.05).Conclusion: Propofol given IT could produce dosedependent analgesic action in the spinal level, and treatment to pain is better than prepair for prevention of pain.

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备注/Memo

备注/Memo:
#通讯作者,男,1959年生,大学本科,教授,研究方向:麻醉与镇痛,Email:tlddtl@163.com
更新日期/Last Update: 2010-05-17