[1]常方方,高明,周云#.奥沙利铂联合顺铂对体外培养BGC823细胞增殖及Bcl2、Bax、Caspase3蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):359-361.
 CHANG Fangfang,GAO Ming,ZHOU Yun.Effects of OXA and DDP on cell proliferation and Bcl2,Bax,Caspase3 expression of BGC823 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):359-361.
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奥沙利铂联合顺铂对体外培养BGC823细胞增殖及Bcl2、Bax、Caspase3蛋白表达的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
359-361
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of OXA and DDP on cell proliferation and Bcl2,Bax,Caspase3 expression of BGC823 cells in vitro
作者:
常方方高明周云#
郑州大学第一附属医院肿瘤内科郑州450052
Author(s):
CHANG FangfangGAO MingZHOU Yun
Department of Oncology,the First Affiliated Hospital,Zhengzhou Univercity,Zhengzhou 450052
关键词:
奥沙利铂顺铂胃癌细胞增殖Bcl2BaxCaspase3BGC823细胞体外
Keywords:
OXADDPgastric carcinomacell proliferationBcl2BaxCaspase3BGC823 cellin vitro
分类号:
R735.2
摘要:
观察奥沙利铂(OXA)联合顺铂(DDP)对胃癌细胞BGC823体外增殖和Bcl2、Bax、Caspase3蛋白表达的影响。方法:采用MTT法测定OXA单药组(3.125、6.250、12.500、25.000mg/L)、DDP单药组(1.5625、3.1250、6.2500、12.5000mg/L)及联合组(3.125/1.5625、6.250/3.1250、12.500/6.2500、25.000/12.5000mg/L)对BGC823细胞的体外生长抑制率。免疫细胞化学法检测联合用药(3.125/1.5625mg/L)和对照细胞Bcl2、Bax、Caspase3蛋白的表达。结果:OXA、DDP单药组及联合组BGC823细胞生长抑制率随着时间和浓度的增加逐渐增高(P均<0.01)。OXA、DDP单药作用于BGC823细胞24、48、72h的IC50值分别为39.5、20.9、10.5mg/L和11.3、7.09、4.07mg/L,2者联合时24、48、72h的IC50值分别为14.68/7.33、6.27/3.14、3.48/1.74mg/L。经OXA(3.125mg/L)、DDP(1.5625mg/L)联合处理48h后,与对照组比较,胃癌细胞Bcl2蛋白表达降低[(37.4±2.4)%vs(73.7±2.2)%],Bax蛋白[(56.4±0.7)%vs(19.9±0.7)%]、Caspase3蛋白[(40.9±1.6)%vs(9.5±1.6)%]表达升高(P均<0.001)。结论:DXA和DDP单药可抑制胃癌细胞BGC823的生长,2者联合对胃癌细胞的抑制作用增强,其机制与通过线粒体途径激活下游的Caspase3诱导细胞凋亡有关。
Abstract:
To observe the effects of OXA and DDP on proliferation and Bcl2,Bax,Caspase3 expression of gastric carcinoma cell BGC823.Methods:The inhibition rate of BGC823 cells was examined by MTT assay after being treated with OXA(3.125,6.250,12.500,25.000 mg/L),DDP(1.562 5,3.125 0,6.250 0,12.500 0 mg/L)and OXA combined with DDP(3.125/1.562 5,6.250/3.125 0,12.500/6.250 0,25.000/12.500 0 mg/L).Bcl2,Bax,Caspase3 expression of BGC823 cell treated with OXA combined wtih DDP was detected by immunohistochemistry assay.Results: The proliferation inhibitive rate of BGC823 cells was in time and dosedependent manner by OXA,DDP single or combined(P<0.01). Compared with single groups, the IC50 of OXA combined with DDP to BGC823 cells was higher(P<0.01). The combined use of OXA and DDP caused Bcl2 upregulation [(37.4±2.4)% vs (73.7±2.2)%] and Bax [(56.4±0.7)% vs (19.9±0.7)%],Caspase3 [(40.9±1.6)% vs (9.5±1.6)%] lowregulation(P<0.001).Conclusion: The proliferation of BGC823 could be inhibited by OXA,DDP single and combined. The combination induces cell apoptosis through mitochondrial pathway.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1959年生,硕士,教授,研究方向:肿瘤基础与临床
更新日期/Last Update: 2010-05-17