[1]陈长英))△,伍钢),张明智).夏枯草提取物体内外对小鼠T淋巴瘤EL4细胞生长的影响[J].郑州大学学报(医学版),2009,(02):380-382.
 CHEN Changying),WU Gang),ZHANG Mingzhi).Inhibition of the extract of Prunella vulgaris L on growth of mouse T Lymphoma EL4 cell in vivo and in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):380-382.
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夏枯草提取物体内外对小鼠T淋巴瘤EL4细胞生长的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
380-382
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Inhibition of the extract of Prunella vulgaris L on growth of mouse T Lymphoma EL4 cell in vivo and in vitro
作者:
陈长英1)2)伍钢1)张明智2)
1)华中科技大学同济医学院附属协和医院肿瘤中心武汉4300232)郑州大学第一附属医院肿瘤科郑州450052
Author(s):
CHEN Changying12)WU Gang1) ZHANG Mingzhi2)
1)The Cancer Center of Union Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 4300232)Department of Oncology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
夏枯草淋巴瘤EL4细胞细胞凋亡
Keywords:
Prunella vulgaris LlymphomaEL4 cellapoptosis
分类号:
R733.4
摘要:
观察夏枯草提取物体内外对小鼠T淋巴瘤EL4细胞生长的影响,探讨夏枯草提取物的抗肿瘤作用机制。方法:用MTT法、DNA琼脂糖凝胶电泳、TUNEL等方法检测夏枯草提取物体外对小鼠T淋巴瘤EL4细胞生长的影响。建立C57BL/6小鼠EL4细胞移植瘤模型,分别给予500、300、100mg/(kg·d)夏枯草提取物,10d后,计算抑瘤率,对肿瘤组织进行形态学观察,并测定其中Bcl2和Bax蛋白的表达情况。结果:夏枯草提取物体外能抑制EL4细胞生长,半数抑制浓度(IC50)为(23.67±3.05)mg/L;夏枯草提取物能诱导EL4细胞凋亡。夏枯草提取物能抑制EL4细胞在C57BL/6小鼠体内的生长,500、300、100mg/(kg·d)剂量组的抑瘤率分别为(39.54±1.83)%、(65.26±1.31)%、(22.30±3.7)%,平均生存时间分别为(25.50±1.87)、(28.50±2.62)、(24.86±2.19)d,均大于空白对照组(P<0.05),移植瘤Bcl2蛋白表达降低,Bax蛋白表达增加。结论:夏枯草提取物在体内外均能抑制EL4细胞的生长,诱导凋亡可能是其抗肿瘤的主要机制之一。
Abstract:
To observe the effects of the extract of Prunella vulgaris L on the growth of mouse T lymphoma cells EL4 in vivo and in vitro,and explore its mechanism of antitumor.Methods:The growth inhibition of EL4 cells cultured with the extract of Prunella vulgaris L was measured by MTT assay,and the apoptosis was detected using agarose gel electrophoresis, TUNEL.The antitumor activity of the extract of Prunella vulgaris L in the experimental EL2 transplantation tumor in C57BL/6 mouse was evaluated.Results:The extract of Prunella vulgaris L inhibited EL4 cells growth in a dosedependent manner,the IC50 values was (23.67±3.05) mg/L.The extract of Prunella vulgaris L could induce EL4 cells apoptosis in vitro.The extract of Prunella vulgaris L could inhibit EL4 cells growth in C57BL/6 mouse,the inhibition rate of the extract of Prunella vulgaris L of 500,300,100 mg/(kg·d) were (39.54±1.83)%,(65.26±1.31)%,(22.30±3.17)%,the mean live time was (25.50±1.87),(28.50±2.63),(24.86±2.19) d, respectively,higher than that of the control group.The expression of Bcl2 protein degraded,that of Bax protein increased in transplantation tumor tissue.Conclusion: The extract of Prunella vulgaris L can inhibit the growth of EL4 cell in vivo and in vitro,inducing apoptosis may be one of the mechanisms of antitumor.

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备注/Memo

备注/Memo:
△女,1963年生,硕士,研究方向:恶性肿瘤的综合治疗,Email:changing000@126.com
更新日期/Last Update: 2010-05-17