[1]申莉),徐宏平),华海婴)#,等.乙酰氧基胡椒酚乙酸酯对人乳癌MCF7细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2009,(02):420-421.
 SHEN Li),XU Hongping),HUA Haiying),et al.Effects of ACA on proliferation and apoptosis of human breast carcinoma cell line MCF7[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(02):420-421.
点击复制

乙酰氧基胡椒酚乙酸酯对人乳癌MCF7细胞增殖及凋亡的影响 ()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年02期
页码:
420-421
栏目:
论著
出版日期:
2009-03-01

文章信息/Info

Title:
Effects of ACA on proliferation and apoptosis of human breast carcinoma cell line MCF7
作者:
申莉1)徐宏平2)华海婴3)张明智1)
1)郑州大学第一附属医院肿瘤科郑州4500522)郑州铁路职业技术学院郑州4500523)郑州大学医药科学研究院郑州450052
Author(s):
SHEN Li1) XU Hongping2)HUA Haiying3) ZHANG Mingzhi1)
1)Department of Oncology,the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Zhengzhou Railway Vocational and Technical College, Zhengzhou 4500523)Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052
关键词:
乙酰氧基胡椒酚乙酸酯MCF7细胞增殖凋亡
Keywords:
1’acetoxychavicol acetatesMCF7 cellsproliferationapoptosis
分类号:
R730.5
摘要:
研究乙酰氧基胡椒酚乙酸酯(ACA)对人乳癌MCF7细胞增殖及凋亡的影响及其作用机制。方法:应用MTT法检测ACA对人乳癌MCF7细胞的增殖抑制作用,观察细胞形态学改变,流式细胞仪分析细胞周期及凋亡率,免疫细胞化学法检测Bcl2、Bax蛋白的表达。结果:ACA剂量依赖性地抑制MCF7细胞增殖,其24h的IC50为(108.57±5.85)μmol/L。100、150μmol/LACA作用后MCF7细胞出现典型的凋亡形态学特征,包括核浓缩致密,细胞皱缩。ACA对MCF7细胞周期分布无明显影响,而终浓度为100、150μmol/L时,出现亚Gl峰。50、100、150μmol/LACA作用后MCF7细胞的Bcl2蛋白表达减少,Bax蛋白表达增加,与对照组比较,差异有统计学意义(P<0.05)。结论:ACA能明显抑制MCF7细胞的增殖,并可通过下调Bcl2和上调Bax蛋白表达诱导其凋亡。
Abstract:
To study the effects and mechanism of 1’acetoxychavicol acetates(ACA) on the proliferation and apoptosis of breast carcinoma cell line MCF7.Methods:Antiproliferative effect of ACA against MCF7 was tested by MTT assay,morphological changes of MCF7 cells were observed by light microscopy,and cell apoptosis percentage and cell cycle phase distribution of MCF7 were measured by flow cytometric assay.The expressions of apoptosisrelated proteins Bcl2 and Bax were determined by immunocytochemical method.Results:A dosedependent proliferation inhibition was demonstrated in ACA and the IC50 for 24 h on MCF7 cells was (108.57 ±5.85) μmol/L. The characteristic morphological changes of apoptosis were observed in cells after treated with ACA,including cell shrinkage and chromatin condensation. There were no significant differences in the percentage of cells treated with ACA in G0/G1,S or G2/M. However, subG1 peak was observed in both the 100 μmol/L and 150 μmol/L treatment groups. With the treatment of ACA,the protein expression of Bcl2 decreased while Bax increased.Conclusion: ACA exhibits the potent proliferation inhibition and apoptosis induction on MCF7 cells, which might be related to the downregulation of Bcl2 expression and upregulation of Bax.

参考文献/References:

[1]Kondo A,Ohigashi H,Murakami A.A potent inhibitor of tumorpromoterinduced EpsteinBarr virus activation, 1'acetoxychavicol acetate from Languas galanga, a traditional Thai condiment[J]. Biosci Biotechnol Biochem, 1993,57:1 344
[2]Ohnishi M, Tanaka T, Makita H, et al. Chemopreventive effect of a xantine oxidase inhibitor, 1'acetoxychavicol acetate, on rat oral carcinogenesis [J]. Jpn J Cancer Res,1996,87(4):349
[3]Moffat J, Hashimoto M, Kojima A, et al. Apoptosis induced by 1'acetoxychavicol acetate in Ehrlich ascites tumor cells is associated with modulation of polyamine metabolism and caspase3 activation [J]. Carcinogenesis, 2000,21(12):2 151
[4]Ito K, Nakazato T, Murakami A, et al. Induction of apoptosis in human myeloid leukemic cells by 1'acetoxychavicol acetate through a mitochondrialand Fasmediated dual mechanism[J]. Clin Cancer Res, 2004,10:2 120
[5]Geng Y J, Libby P. Progression of atheroma: a struggle between death and procreation[J]. Arterioscler Thromb Vasc Biol, 2002,22:1 370
[6]Ito K, Nakazato T, XianMJ, et al. 1′Acetoxychavicol acetate is a novel nuclear factor kappaB inhibitor with significant activity against multiple myeloma in vitro and in vivo[J].Cancer Res,2005,65(10):4 417
[7]Cheryl T Campbell, Misty Prince, Greg M Landry, et al. Proapoptotic effects of 1'acetoxychavicol acetate in human breast carcinoma cells [J].Toxicology Letters,2007,173:151

相似文献/References:

[1]权松霞),张振中),邵彦江),等.脂质体介导的hTERT PEI/ASODN缩合体对乳癌MCF7细胞生长及hTERT表达的影响[J].郑州大学学报(医学版),2010,(04):554.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):554.
[2]崔莹),孙淼淼),崔黎),等.RNA干扰对MCF7细胞eIF4E表达的影响*[J].郑州大学学报(医学版),2011,(03):364.
 CUI Ying,SUN Miaomiao,CUI Li,et al.Effects of RNAi on expression of eukaryotic initiation factor 4E in MCF7 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(02):364.

备注/Memo

备注/Memo:
#通讯作者,女,1957年生,副研究员,研究方向:肿瘤药理基础与新药研究,Email:hhylu@yahoo.com
更新日期/Last Update: 2010-05-17