[1]张卫星),韩广业),王瑞)﹟,等.大鼠精原干细胞的分离纯化*[J].郑州大学学报(医学版),2009,(03):497-500.
 ZHANG Weixing),HAN Guangye),WANG Rui),et al.Separation and purification of rat spermatogonial stem cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):497-500.
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大鼠精原干细胞的分离纯化* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
497-500
栏目:
应用研究
出版日期:
2009-05-01

文章信息/Info

Title:
Separation and purification of rat spermatogonial stem cells
作者:
张卫星1)韩广业1)王瑞1)李松1)韩雪飞2)
1)郑州大学第一附属医院泌尿外科;河南省高等学校临床医学重点学科开放实验室;郑州大学肿瘤分子外科研究所郑州4500522)郑州大学干细胞研究中心郑州450052
Author(s):
ZHANG Weixing1)HAN Guangye1)WANG Rui1)LI Song1)HAN Xuefei2)
关键词:
大鼠精原干细胞分离纯化
Keywords:
rat spermatogonial stem cellseparationpurification
分类号:
R698+.2;R321.1
摘要:
探讨分离纯化大鼠精原干细胞(SSCs)的方法。方法:取大鼠睾丸组织,通过①机械法+两步酶法,②机械法+两步酶法+Percoll分离法,③机械法+两步酶法+Percoll分离法+差速贴壁法得到细胞悬液,进行培养观察。采用免疫组织化学方法鉴定,台盼蓝排斥实验及流式细胞仪分别检测细胞活率与纯度。结果:①、②、③法所得细胞为SSCs,所得SSCs活率分别为(93.26±1.06)%,(93.50±0.85)%和(94.73±0.82)%,差异无统计学意义(P>0.05);①、 ②、③法所得SSCs纯度呈增高趋势(P<0.05)。结论:3种方法在分离纯化过程中对SSCs活率的影响无明显差异。①法能够分离出较高纯度的SSCs,结合Percoll分离法能够使SSCs纯度提高,再结合差速贴壁法能够进一步富集SSCs。

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备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助项目20060037;河南省自然科学基金资助项目72102310025;河南省医学科技攻关基金资助项目200703014#通讯作者,男,1963年生,硕士,教授,研究方向:男科学,Email:huzi918@hotmail.com
更新日期/Last Update: 2010-05-28