[1]邢莲梅),赵玉林)#,余文发),等.尼莫地平对rhTGFα诱导的人喉鳞癌Hep2细胞增殖及RNA编辑酶1mRNA表达的影响*[J].郑州大学学报(医学版),2009,(03):537-538.
 XING Lianmei),ZHAO Yulin),YU Wenfa),et al.Effects of nimodipin on proliferation induced by rhTGFα and mRNA expression of RNAdependent adenosine deaminase 1 in human larynx cancer Hep2 cell lines[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):537-538.
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尼莫地平对rhTGFα诱导的人喉鳞癌Hep2细胞增殖及RNA编辑酶1mRNA表达的影响* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
537-538
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Effects of nimodipin on proliferation induced by rhTGFα and mRNA expression of RNAdependent adenosine deaminase 1 in human larynx cancer Hep2 cell lines
作者:
邢莲梅1)赵玉林2)#余文发2)张军辉2)
1)新乡市第一人民医院耳鼻咽喉头颈外科新乡4530002)郑州大学第一附属医院耳鼻咽喉头颈外科郑州450052
Author(s):
XING Lianmei1)ZHAO Yulin2)YU Wenfa2)ZHANG Junhui2)
1)Department of Otolaryngology, the First People's Hospital of Xinxiang, Xinxiang 4530002)Department of Otolaryngology,the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
喉肿瘤钙通道RNA编辑酶1
Keywords:
To investigate the effects of calcium channel antagonist nimodipin on the proliferation induced by rhTGFα and the mRNA expression of RNAdependent adenosine deaminase1(ADAR1)in human larynx cancer Hep2 cell lines.Methods:Hep2 cell was used as an investigat
分类号:
R739.65
摘要:
研究钙离子通道阻滞剂尼莫地平(ND)对rhTGFα诱导的人喉鳞癌Hep2细胞增殖及RNA编辑酶1(ADAR1)表达的影响。方法:采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度ND(1.0×10-6 mol/L、1.0×10-5 mol/L、1.0×10-4 mol/L)对rhTGFα促Hep2细胞增殖的影响;用逆转录聚合酶链反应(RTPCR)检测钙通道阻滞前后Hep2细胞ADAR1 mRNA的表达。结果:ND呈剂量依赖性地抑制了rhTGFα诱导的Hep2细胞增殖;Hep2细胞钙通道被ND阻滞后ADAR1 mRNA相对表达量较阻滞前明显降低(P均<0.05)。结论:阻滞Hep2细胞钙通道可抑制Hep2细胞增殖;Hep2细胞ADAR1 mRNA的表达可能和钙通道密切相关。
Abstract:
To investigate the effects of calcium channel antagonist nimodipin on the proliferation induced by rhTGFα and the mRNA expression of RNAdependent adenosine deaminase1(ADAR1)in human larynx cancer Hep2 cell lines.Methods:Hep2 cell was used as an investigative target. The effects of different concentration ND on the proliferation of Hep2 cell enhanced by rhTGFα was evaluated by MTT assay.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省教育厅自然科学基金资助项目2007320076;河南省医学高新技术发展扶持项目20060011#通讯作者,男,1965年生,博士,教授,主任医师,研究方向:头颈肿瘤的防治,Email:zhaoyulinmail@163.com
更新日期/Last Update: 2010-05-28