[1]赵先兰△,张会利,饶燕玲.子宫颈鳞状细胞癌组织中DNMT1mRNA表达和Ecad甲基化检测[J].郑州大学学报(医学版),2009,(03):558-561.
 ZHAO Xianlan,ZHANG Huili,RAO Yanling.Detection of DNMT1 mRNA and Ecadherin hypermethylation in cervical cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):558-561.
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子宫颈鳞状细胞癌组织中DNMT1mRNA表达和Ecad甲基化检测()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
558-561
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Detection of DNMT1 mRNA and Ecadherin hypermethylation in cervical cancer
作者:
赵先兰张会利饶燕玲
郑州大学第一附属医院妇产科郑州450052
Author(s):
ZHAO Xianlan ZHANG Huili RAO Yanling
Department of Gynecology and Obstetrics,the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
宫颈鳞状细胞癌DNA甲基化转移酶1上皮钙粘附素甲基化甲基化特异性PCR
Keywords:
cervical carcinoma DNMT1 Ecad methylation MSP
分类号:
R711.74
摘要:
探讨宫颈鳞状细胞癌组织中DNA甲基化转移酶(DNMT1) mRNA的表达和上皮钙粘附素(Ecadherin )的甲基化状况,分析其与宫颈鳞状细胞癌发生发展的关系。方法:应用原位杂交技术检测20例正常宫颈、60例CIN(CINⅠ、CINⅡ、CINⅢ各20例)和40例宫颈鳞状细胞癌组织中DNMT1 mRNA的表达情况;利用甲基化特异性PCR(methylation specific polymerase chain reaction,MSP)方法检测Ecad基因启动子区甲基化状况,分析宫颈鳞状细胞癌中DNMT1mRNA的表达与Ecad启动子区甲基化状况及2者之间的相关性。结果:DNMT1 mRNA在正常宫颈、CIN和宫颈鳞状细胞癌组织中表达逐渐增加,差异有统计学意义(P<0.05);DNMT1 mRNA在CINⅠ、CINⅡ、CINⅢ组织中表达的差异有统计学意义(P<0.05)。

参考文献/References:

[1]Robertson Kn,Uzvolgyi E,Liang G, et al. The human DNA methyltransferases(DNMTs)1,3a and 3b:coordinate mRNA expresstion in normal tissues and overexpresstion in tumors[J].Nueleie Aeids Res,1999, 27(11):2 291
[2]Anastasiadis PZ, Reynolds AB. The P120 catenin family: complex roles in adhesion,signaling and cancer[J]. J Cell Sci,2000,113(Pt8):1 319
[3]Zuo S, Luo J, Liu M,et al. Suppressing effects of downregulating DNMT1 and DNMT3b expression on the growth of human cholangiocarcinoma cell line[J]. J Huazhong Univ Sci Technolog Med Sci,2008, 28(3):276
[4]Liao X, Siu MK, Chan KY,et al. Hypermethylation of RAS effector related genes and DNA methyltransferase 1 expression in endometrial carcinogenesis[J]. Int J Cancer,2008, 123(2):296
[5]Ding WJ, Fang JY, Chen XY,et al. The expression and clinical significance of DNA methyltransferase proteins in human gastric cancer[J]. Dig Dis Sci,2008, 53(8):2 083
[6]Fan H, Zhao Z, Quan Y,et al. DNA methyltransferase 1 knockdown induces silenced CDH1 gene reexpression by demethylation of methylated CpG in hepatocellular carcinoma cell line SMMC7721[J].Eur J Gastroenterol Hepatol,2007, 19(11):952
[7]Sawada M, Kanai Y, Arai E, et al. Increased expression of DNA methyltransferase 1 (DNMT1) protein in uterine cervix squamous cell carcinoma and its precursor lesion[J]. Cancer Lett, 2007, 251(2):211
[8]Etoh T, Kanai Y, Ushijima S, et al. Increased DNA Methyltransferase 1(DNMT1) protein expression correlates significantly with poorer tumor differentiation and frequent DNA hypermethylation of multiple CpG islands in gastric cancers[J].Am J Pathol, 2004, 164(2):689

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备注/Memo

备注/Memo:
△女,1965年生,教授,主任医师,研究方向:妇科肿瘤,Email:13623812129@139.com
更新日期/Last Update: 2010-05-28