[1]陈志敏,史惠蓉#.子宫内膜腺癌组织中核干细胞因子和增殖细胞核抗原的表达[J].郑州大学学报(医学版),2009,(03):562-564.
 CHEN Zhimin,SHI Huirong.Expressions of Nucleostemin and PCNA in endometrial carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):562-564.
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子宫内膜腺癌组织中核干细胞因子和增殖细胞核抗原的表达 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
562-564
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Expressions of Nucleostemin and PCNA in endometrial carcinoma
作者:
陈志敏史惠蓉#
郑州大学第一附属医院妇产科郑州450052
Author(s):
CHEN Zhimin SHI Huirong
Department of Obstetrics and Gynecology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
核干细胞因子增殖细胞核抗原子宫内膜肿瘤
Keywords:
Nucleostemin PCNA endometrial neoplasms
分类号:
R737.33
摘要:
探讨子宫内膜腺癌(EMC)组织中核干细胞因子(NS)和增殖细胞核抗原(PCNA)表达与临床病理特性、预后的关系。方法:采用RTPCR方法检测15例正常子宫内膜、26例子宫内膜增生过长及52例子宫内膜癌标本中NS mRNA的表达情况,应用免疫组织化学SP法检测PCNA蛋白的表达情况。结果:EMC与正常子宫内膜和子宫内膜增生过长组织间NS mRNA和PCNA蛋白的表达差异有统计学意义(P<0.05),且二者的表达与EMC的病理分级、临床分期有关(P<0.05),PCNA蛋白的表达还与EMC的浸润深度有关(P<0.05)。在子宫内膜腺癌组织中NS mRNA和PCNA的表达呈正相关(rs=0.609,P<0.05)。
Abstract:
To analyze expressions of Nucleostemin(NS) and PCNA in endometrial carcinomas and investigate their clinical pathological correlations and prognostic value.Methods:Semiquantitative reverse transcriptasepolymerase chain (RTPCR) was used to detect expression of NS mRNA in samples of normal endometria, endometrial hyperplasia and endometrial carcinoma. Immunohistochemistry was applied to examine expression of PCNA protein in the paraffin sections of these samples.

参考文献/References:

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[2]Tsai RY, McKay RD. A multistep, GTPdriven mechanism controlling the dynamic cycling of nucleostemin[J]. J Cell Biol, 2005,168(2):179
[3]Jafarnejad SM,Mowla SJ,Matin MM.Knockingdown the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53independent manner[J].Cell Prolif,2008,41(1):28
[4]Bernardi R, Pandolfi PP. The nucleolus: at the stem of immortality[J]. Nat Med, 2003,9(1):24
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[6]Sijin L, Ziwei C, Yajun L, et al. The effect of knockingdown nucleostemin gene expression on the in vitro proliferation and in vivo tumorigenesis of HeLa cells[J]. J Exp Clin Cancer Res, 2004, 23(3): 529
[7]张功员, 尹磊, 李晟磊,等.食管鳞状细胞癌组织中核干细胞因子蛋白的表达[J].郑州大学学报:医学版, 2007, 42(6):1 088
[8]Bravo R, Frank R, Blundell PA, et al. Cyclin/PCNA is the auxiliary protein of DNA polymerasedelta[J]. Nature,1987,326(6 112):515
[9]苏联珍, 吴静. PTEN和PCNA在子宫内膜癌组织中的表达[J]. 西安交通大学学报:医学版,2007,28(2):187

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备注/Memo

备注/Memo:
#通讯作者,女,1956年生,博士,教授,研究方向:妇科肿瘤,Email:huirongshi@yahoo.com.cn
更新日期/Last Update: 2010-05-28