[1]王曼莉,滕军放#,赵莘瑜,等.胰岛素对氧糖剥夺诱导大鼠脑神经元损伤的改善作用[J].郑州大学学报(医学版),2009,(03):565-567.
 WANG Manli,TENG Junfang,ZHAO Xinyu,et al.Insulin prevents OGD/Rinduced injury in cortical neuron of rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):565-567.
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胰岛素对氧糖剥夺诱导大鼠脑神经元损伤的改善作用 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
565-567
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Insulin prevents OGD/Rinduced injury in cortical neuron of rats
作者:
王曼莉滕军放#赵莘瑜周艳丽
郑州大学第一附属医院神经内科郑州450052
Author(s):
WANG Manli TENG Junfang ZHAO Xinyu ZHOU Yanli
Department of Neurology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
大鼠神经元氧糖剥夺/复糖复氧胰岛素生长相关蛋白脑源性神经营养因子
Keywords:
rat neuron sugar oxygen deprivation/rehabilitation of sugaroxygen insulin GAP43BDNF
分类号:
R965
摘要:
探讨胰岛素对氧糖剥夺诱导新生大鼠脑皮质神经元损伤的改善作用。方法:将培养7 d的大鼠皮质神经元随机分为3组,A组为正常对照组,B组采用氧糖剥夺/复糖复氧(OGD/R)处理,C组采用胰岛素预处理加OGD/R处理,B、C组在OGD/R后0、1、6、12、24 h各时间点,以MTT法检测细胞活性,采用脱氧尿嘧啶缺口末端标记法检测神经元凋亡情况,采用免疫细胞化学方法检测生长相关蛋白(GAP43)、脑源性神经营养因子(BDNF)的表达。结果:①B组的神经元细胞活性较A组明显下降,C组细胞活性明显高于B组,3组比较差异有统计学意义(P<0.05)。②B组凋亡神经元细胞明显多于正常对照组,OGD/R后6、12、24 h,C组凋亡细胞数目明显少于B组,3组比较差异有统计学意义(P<0.05)。③B组GAP43、BDNF的表达较正常对照组明显增加,OGD/R后6、12、24 h,C组GAP43、BDNF表达较B组明显增加。
Abstract:
To explore the protective effects of insulin on injury induced by OGD/R in neonatal rats.Methods:Cortical neurons cultured for 7 days were randomly divided into A (normal control group), B (OGD/R alone), C (pretreatment with insulin) 3 groups. Then detect cell survival rate by MTT colorimetry, count the apoptosis neurons by TUNEL(TdT mediated dUTP nick end labeling), observe the expression of GAP43, BDNF using Immunocytochemical on each time point after OGD/R 0 h,1 h, 6 h, 12 h, 24 h of each group.

参考文献/References:

[1]Zhu CZ, Auer RN. Optimal blood glucose levels while using insulin to minimize the size of infarction in focal cerebral ischemia[J].J Neurosury,2004,101(4):664
[2]Venable AM, Mitalipova M, Lyons I, et al. Lectin binding profiles of SSEA4 enriched,pluripotent human embryonic stem cell surfaces[J]. BMC Dev Biol, 2005,5:15
[3]Muotri AR, Chu VT, Marchetto MC, et al. Somatic mosaicism in neuronal precursor cells mediated by L1 retrotransposition [J]. Nature,2005,435(7 044):903
[4]Van Den Berghe G,Schoonheydt K,Becx P,et al.Insulin therapy protects the central and peripheral nervous system of intensive care patients[J].Neurology,2005,64(8):1 348
[5]Marklund N, Bareyre FM, Royo NC, et al.Cognitive outcome following brain injury and treatment with an inhibitor of NogoA in association with an attenuated downregulation of hippocampal growthassociated protein43 expression[J].J Neurosurg,2007,107(4):844
[6]Pillai A, VeerananKarmegam R, Dhandapani KM, et al.Cystamine prevents haloperidolinduced decrease of BDNF/TrkB signaling in mouse frontal cortex[J].J Neurochem,2008,107(4):941
[7]Byerly MS, Simon J, LebihanDuval E, et al.Effects of BDNF, T3, and corticosterone on expression of the hypothalamic obesity gene network in vivo and in vitro[J].Am J Physiol Regul Integr Comp Physiol,2009,296(4):R1 180

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备注/Memo

备注/Memo:
#通讯作者,男,1960年生,教授,主任医师,研究方向:脑血管病和睡眠障碍,Email:tjf6666@yahoo.com.cn
更新日期/Last Update: 2010-05-28