[1]杨东斌,宋来君#,杨波,等.不同人羊膜间充质干细胞冻存液冻存细胞效果比较[J].郑州大学学报(医学版),2009,(03):568-571.
 YANG Dongbin,SONG Laijun,YANG Bo,et al.Preliminary study on cryopreservation of human amniotic mesenchymal stem cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):568-571.
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不同人羊膜间充质干细胞冻存液冻存细胞效果比较 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
568-571
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Preliminary study on cryopreservation of human amniotic mesenchymal stem cells
作者:
杨东斌宋来君#杨波李祥生
郑州大学第一附属医院神经外科郑州450052
Author(s):
YANG DongbinSONG LaijunYANG BoLI Xiangsheng
Department of Neurosurgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
人羊膜间充质干细胞冻存复苏冻存液
Keywords:
human amnioticmesenchymal stem cellsfrozenrecovery of frozenfrozen liquid
分类号:
Q421
摘要:
筛选冻存人羊膜间充质干细胞效果较好的冻存液。方法:①不同配方的细胞冻存液对细胞冻存效果的影响。人羊膜间充质干细胞培养至第三代后分为10组,分别加入以下10种不同组成的冻存液。A组:70%DMEM/F12+20%胎牛血清+10% DMSO;B组: 60%DMEM/F12+30%胎牛血清+10% DMSO;C组: 40%DMEM/F12+50%胎牛血清+10% DMSO;D组: 90%胎牛血清+10% DMSO;E组: 70%DMEM/F12+20%胎牛血清+10%甘油;F组: 60%DMEM/F12+30%胎牛血清+10%甘油;G组: 40%DMEM/F12+50%胎牛血清+10%甘油;H: 90%胎牛血清+10%甘油;I组: 90%DMEM/F12+10%DMSO;J组: 90%DMEM/F12+10%甘油。调整细胞密度为5×106 mL-1,放入程序降温盒中,入-80 ℃低温冰箱保存。1个月后复苏,MTT法筛选出吸光光度值较高的一种冻存液。②相同血清含量细胞冻存液对细胞冻存效果的影响。含体积分数为50%胎牛血清的细胞冻存液分3组保存细胞。

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1952年生,教授,主任医师,研究方向:神经外科的基础与临床,Email:laijunsong@126.com
更新日期/Last Update: 2010-05-28