[1]王庆祝),张贺),秦贵军)#,等.吡咯烷二硫代氨基甲酸酯和IL18对大鼠肾小球系膜细胞分泌Ⅳ型胶原蛋白和TNFαmRNA的影响[J].郑州大学学报(医学版),2009,(03):588-590.
 WANG Qingzhu),ZHANG He),QIN Guijun),et al.Effect of PDTC and interleukin18 on secretion of COⅣ and expression of TNFα mRNA in rat mesangial cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):588-590.
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吡咯烷二硫代氨基甲酸酯和IL18对大鼠肾小球系膜细胞分泌Ⅳ型胶原蛋白和TNFαmRNA的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
588-590
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Effect of PDTC and interleukin18 on secretion of COⅣ and expression of TNFα mRNA in rat mesangial cells
作者:
王庆祝1)张贺2)秦贵军2)#岳欣阁2)余勤2)董义光2)
1)郑州大学第一附属医院核医学科郑州4500522)郑州大学第一附属医院内分泌科郑州450052
Author(s):
WANG Qingzhu1) ZHANG He2) QIN Guijun2) YUE Xinge2) YU Qin2)etc
1)Department of Nuclear Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
吡咯烷二硫代氨基甲酸酯白细胞介素18系膜细胞细胞外基质肿瘤坏死因子α大鼠
Keywords:
PDTC IL18 mesangial cell extracellular matrix TNFαrat
分类号:
R587.1
摘要:
研究吡咯烷二硫代氨基甲酸酯(PDTC)和白细胞介素18 (IL18)对肾小球系膜细胞(MC)分泌Ⅳ型胶原蛋白和表达肿瘤坏死因子α(TNFα)的作用。 方法:将MC随机分为6组:A组为正常对照组,B组为加入25 μg/L IL18组,C组为加入50 μg/L IL18组,D、E、F组分别在A、B、C组基础上加入PDTC(100 μmol/L),均在高糖环境下培养48 h。采用ELISA检测Ⅳ型胶原蛋白(COⅣ)和层粘连蛋白(LN),RTPCR检测MC TNFα mRNA的表达。结果:高糖环境下,25 μg/L、50 μg/L 的IL18均能促进MC分泌COⅣ和LN,表达TNFα mRNA (P<0.05),随IL18浓度增加,MC分泌COⅣ的量及表达TNFα mRNA有升高趋势(P<0.05)。PDTC能抑制MC分泌COⅣ和LN及表达TNFα mRNA。结论:IL18可能通过NFκB激活途径影响MC分泌COⅣ、LN和TNFα mRNA的表达而参与糖尿病肾病的发生、发展。
Abstract:
To study the effects of PDTC and interleukin18(IL18)on COⅣ secretion and the expression of TNFα in glomerular mesangial cells(MC).Methods:MCs were randomly divided into 6 groups:normal control(group A),concentration of 25 μg/L IL18(group B),concentration of 50 μg/L IL18(group C);at the base of group A,B,C,adding PDTC(100 μmol/L)was group D,E,F.MCs in the 6 groups were cultured for 48 h in high glucose environment.The levels of the type Ⅳ collagen (COⅣ)and laminin(LN) were determined by ELISA. The expression of TNFα mRNA was detected with RTPCR.

参考文献/References:

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[2]Nakamura A,Shikata K, Hiramatsu M,et al. Serum interleukin18 levels are associated with nephropathy and atherosclerosis in Japanese patients with type 2 diabetes[J]. Diabetes Care, 2005,28(12):2 890
[3]谌贻璞,高进,邢宝才,等.肾小球系膜细胞的培养[J].北京医科大学学报,1989,21(4):335
[4]刘华锋,陈孝文,吴瑗,等.原发性肾小球肾炎患者肾组织白介素18的表达[J].中华肾脏病杂志,2003,19(2):127
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备注/Memo

备注/Memo:
#通讯作者,男,1965年生,博士,教授,研究方向:糖尿病的防治,Email:hyqingj@zzu.edu.cn
更新日期/Last Update: 2010-05-28