[1]臧卫周△,杨红旗,徐军,等.姜黄素对缺氧复氧损伤PC12细胞的影响[J].郑州大学学报(医学版),2009,(03):594-596.
 ZANG Weizhou,YANG Hongqi,XU Jun,et al.Effects of curcumin on PC12 cells impaired by mimic ischemia and reperfusion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):594-596.
点击复制

姜黄素对缺氧复氧损伤PC12细胞的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
594-596
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Effects of curcumin on PC12 cells impaired by mimic ischemia and reperfusion
作者:
臧卫周杨红旗徐军王文霞
河南省人民医院老年病研究中心神经内科郑州450003
Author(s):
ZANG Weizhou YANG Hongqi XU Jun WANG Wenxia
Department of Neurology,Research Center of Geriatry, Henan Provinicial People's Hospital,Zhengzhou 450003
关键词:
姜黄素PC12细胞缺氧复氧Bcl2BaxCaspase3
Keywords:
curcumin PC12 cellischemia and reperfusionBcl2 Bax Caspase3
分类号:
R282.71
摘要:
观察姜黄素对缺氧复氧致PC12细胞损伤的保护作用,并探讨其可能的机制。方法:实验分为对照组、缺氧复氧组(IR组)和低、中、高剂量姜黄素组 (C1、C2和C3组)。C1、C2和C3组分别应用20、40、100 μmol/L的姜黄素预处理。IR组、C1、C2和C3组均给予缺氧复氧损伤处理。通过MTT比色法检测细胞存活率,并应用Western blotting方法检测细胞内Bcl2、Bax、Caspase3蛋白含量的变化。结果:与对照组相比,IR组细胞存活率降低(P<0.05);与IR组比较,C2、C3组细胞存活率升高(P<0.05)。姜黄素可促进缺氧复氧诱导的PC12细胞Bcl2表达增加,抑制Bax和Caspase3蛋白的表达,其作用呈剂量依赖性。结论:姜黄素对缺氧复氧导致的PC12细胞损伤有保护作用,这一作用与其抗凋亡特性有关。
Abstract:
To observe the effects of curcumin on PC12 cells impaired by the mimic ischemia and reperfusion(IR), and to investigate the possible mechanism.Methods:PC12 cells were grouped as following: the control group,IR group, and curcumin groups(C1, C2 and C3), which were pretreated with curcumin of 20 μmol/L, 40 μmol/L and 100 μmol/L,separately.IR group,C1,C2 and C3 groups were treated with IR. Cellular survivability was measured by MTT colorimetry.

参考文献/References:

[1]Xu Y, Ku B, Tie L, et al. Curcumin reverses the effects of chronic stress on behavior, the HPA axis, BNDF expression and phosphorylation of CREB[J]. Brain Res, 2006, 1 122(1):56
[2]张书刚, 胡梅, 周其冈, 等. 姜黄素对血管性痴呆大鼠学习记忆的影响[J]. 南京医科大学学报:自然科学版, 2006,26(4): 246
[3]唐小卿, 聂亚雄, 冯鉴强, 等. 姜黄素对H2O2损伤PC12细胞的保护作用[J]. 中国药理学通报, 2004, 20(6): 672
[4]Grutter MG. Caspases: key players in programmed cell death[J]. Curr Opin Struct Biol, 2000, 10(6):649
[5]Nicholls DG, Budd SL. Mitochondria and neuronal survival[J]. Physiol Rev, 2000,80(1):315
[6]Tatton WG, Olanow CW. Apoptosis in neurodegenerative diseases: the role of mitochondria[J]. Biochim Biophys Acta, 1999, 1 410(2):195
[7]Chen J, Graham SH, Chan PH, et al. bcl2 is expressed in neurons that survive focal ischemia in the rat[J]. Neuroreport, 1995,6(2):394
[8]Lawrence MS, Ho DY,Sun GH,et al.Overexpression of Bcl2 with herpes simplex virus vectors protects CNS neurons against neurological insults in vitro and in vivo[J]. J Neurosci,1996,16(2):486
[9]Cory S,Adams JM.The Bcl2 family: regulators of cellular lifeordeath switch[J].Nat Rev Cancer,2002,2(9):647
[10]程虹, 王芳, 刘惟莞, 等. 姜黄素对培养乳鼠心肌细胞损伤的抗氧化作用[J]. 广东药学院学报, 2005,21(6):703
[11]陈静,唐小卿,胡志苹,等.姜黄素对MPP+诱导PC12细胞凋亡的影响[J].中国药理学通报,2005,21(3):335
[12]陈静, 唐二虎, 潘渊明,等. 姜黄素对MPP+诱导PC12细胞凋亡的保护作用及其与iNOS的关系[J]. 中山大学学报:医学科学版, 2005, 26(4):424

相似文献/References:

[1]舒建昌△,朱海燕,吴海恩,等.姜黄素对人肝星状细胞形态学的影响*[J].郑州大学学报(医学版),2009,(06):1141.
 SHU Jianchang,ZHU Haiyan,WU Haien,et al.Effects of curcumin on morphology of human hepatic stellate cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):1141.
[2]何航),华海婴)#,戈士文),等.姜黄素对四氯化碳诱导大鼠肝纤维化的影响*[J].郑州大学学报(医学版),2009,(05):986.
 HE Hang),HUA Haiying),GE Shiwen),et al.Effect of curcumin on rat hepatic fibrosis induced by carbon tetrachoride[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):986.
[3]龚全峰)△,杨红旗).烷丙苯胺对6羟基多巴胺致PC12细胞损伤的改善作用[J].郑州大学学报(医学版),2009,(05):1003.
 GONG Quanfeng),YANG Hongqi).Neuroprotective effects of deprenyl against 6hydroxydopamine inducing neurotoxicity in PC12 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):1003.
[4]潘帧婕,曹靖,李鸣,等.谷氨酸钠对PC12细胞钠离子通道蛋白1.7表达的影响*[J].郑州大学学报(医学版),2013,(05):591.
 PAN Zhenjie,CAO Jing,LI Ming,et al.Effects of sodium glutamate on sodium channel protein 1.7 subunit expression in PC12 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(03):591.
[5]齐跃东△,张晓坚.姜黄素对多囊卵巢综合征大鼠代谢紊乱的影响[J].郑州大学学报(医学版),2014,(04):571.
 QI Yuedong,ZHANG Xiaojian.Effects of curcumin on rats with polycystic ovary syndrome[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(03):571.
[6]任京力),林玲),王雁梅),等.氯通道阻断剂DIDS对过氧化氢诱导PC12细胞凋亡的影响*[J].郑州大学学报(医学版),2015,(01):17.
 REN Jingli),LIN Ling),WANG Yanmei),et al.Effect of chloride channel blocker DIDS on apoptosis of PC12 cells induced by hydrogen peroxide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(03):17.
[7]潘 峰△.姜黄素对鱼藤酮损伤的多巴胺能神经元的保护作用[J].郑州大学学报(医学版),2016,(02):271.
[8]顾 然,王 露,唐 蔓,等.miR-422a过表达联合EPO对H2O2诱导的PC12细胞活力、凋亡及PI3K/AKT信号通路的影响[J].郑州大学学报(医学版),2019,(04):593.[doi:10.13705/j.issn.1671-6825.2018.11.079]
 GU Ran,WANG Lu,TANG Man,et al.Effects of miR-422a overexpression combined with EPO on viability, apoptosis and PI3K/AKT signaling pathway of PC12 cells induced by H2O2[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(03):593.[doi:10.13705/j.issn.1671-6825.2018.11.079]

备注/Memo

备注/Memo:
△男,1974年生,硕士,主治医师,研究方向:阿尔茨海默病的基础和临床
更新日期/Last Update: 2010-05-28