[1]徐宏平),何航)#.水飞蓟宾对人肝星状细胞LX2凋亡及α1(I)胶原mRNA表达的影响[J].郑州大学学报(医学版),2009,(03):597-598.
 XU Hongping),HE Hang).Effects of silibinin on apoptosis and α1(I) collagen mRNA expression in human hepatic stellate cells LX2[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):597-598.
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水飞蓟宾对人肝星状细胞LX2凋亡及α1(I)胶原mRNA表达的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
597-598
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Effects of silibinin on apoptosis and α1(I) collagen mRNA expression in human hepatic stellate cells LX2
作者:
徐宏平1)何航2)#
1)郑州铁路职业技术学院郑州4500522)河南中医学院实验中心免疫室郑州450008
Author(s):
XU Hongping1) HE Hang2)
1)Zhengzhou Railway VocationalTechnical College,Zhengzhou 4500522)Department of Immunology, Laboratory Center for Medical Sciences, Henan College of Traditional Chinese Medicine,Zhengzhou 450008
关键词:
水飞蓟宾肝星状细胞凋亡胶原ⅠmRNA
Keywords:
silibinin hepatic stellate cell apoptosis α1(I) collagen mRNA
分类号:
R285.5
摘要:
观察水飞蓟宾对人肝星状细胞LX2凋亡与α1(I) 胶原mRNA表达的影响。方法:人肝星状细胞株LX2分为对照组与实验组,对照组以体积分数10%胎牛血清处理,实验组分别以体积分数10%胎牛血清和终浓度分别为6.25 mg/L、12.5 mg/L、 25 mg/L、50 mg/L的水飞蓟宾处理48 h后,应用流式细胞仪Annexin/PI双染方法检测细胞凋亡,应用RTPCR检测各组细胞α1(I)胶原 mRNA的表达。结果:12.5 mg/L、 25 mg/L、50 mg/L水飞蓟宾可促进LX2细胞凋亡,细胞凋亡率随水飞蓟宾浓度增加而升高(P<0.05);不同浓度的水飞蓟宾明显降低LX2细胞α1(I)胶原mRNA的表达(P<0.05)。结论:水飞蓟宾可促进肝星状细胞凋亡,抑制α1(I)胶原mRNA的表达。
Abstract:
To investigate the effects of silibinin on apoptosis and α1(I) collagen mRNA expression in cultured human hepatic stellate cells (HSCs) LX2 in vitro.Methods:HSCs were divided into control group and experiment group. HSCs in the control group were treated with 10% fetal calf serum (FCS)/DMEM and HSCs in the experiment group were treated with 10% FCS/DMEM and silibinin with different concentrations (6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L) for 48 hours.

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备注/Memo

备注/Memo:
#通讯作者,女,1971年生,硕士,讲师,研究方向:肝病药理,Email:hehang03961971@163.com
更新日期/Last Update: 2010-05-28