[1]韩德昌)),梁建锋),韩新光)#.LY294002对舌鳞状细胞癌细胞增殖及PTEN蛋白表达的影响[J].郑州大学学报(医学版),2009,(03):613-615.
 HAN Dechang),LIANG Jianfeng),HAN Xinguang).Effect of LY294002 on human tongue squamous carcinoma cell proliferation and PTEN protein expression in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):613-615.
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LY294002对舌鳞状细胞癌细胞增殖及PTEN蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
613-615
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Effect of LY294002 on human tongue squamous carcinoma cell proliferation and PTEN protein expression in vitro
作者:
韩德昌1)2)梁建锋1)韩新光1)#
1)郑州大学第一附属医院口腔颌面外科郑州4500522)郑州人民医院口腔科郑州450053
Author(s):
HAN Dechang12) LIANG Jianfeng1) HAN Xinguang1)
1)Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Stomatology, the People’s Hospital of Zhengzhou,Zhengzhou 450052
关键词:
PI3K/Akt通路LY294002Tca8113细胞细胞增殖PTEN蛋白
Keywords:
PI3K/Akt signal pathway LY294002 Tca8113 cell cell proliferation PTEN protein
分类号:
R739.86
摘要:
探讨PI3K/Akt通路特异性抑制剂LY294002对体外培养的人舌鳞状细胞癌Tca8113细胞增殖活性和PTEN蛋白表达的影响。方法:取处于对数生期的Tca8113细胞以2.5×104 mL-1的浓度接种于96孔板,加入不同浓度(5、10、20、40μmol/L)的 LY294002,以等体积的培养液为空白对照组,分别作用24、48、72、96 h,MTT法检测细胞增殖抑制率;免疫细胞化学法检测浓度为40 μmol/L实验组的PTEN蛋白表达。结果:随药物浓度的增加及作用时间的延长,实验组Tca8113细胞的细胞增殖抑制率逐渐增大,LY294002抑制作用与浓度及作用时间呈依赖关系;浓度为40 μmol/L实验组的PTEN蛋白阳性表达率( 82.5%)高于对照组(24.3%),差异有统计学意义(P<0.05)。结论:LY294002 对体外培养的人舌鳞状细胞癌Tca8113细胞增殖有抑制作用,并可能促使PTEN蛋白表达。
Abstract:
To investigate the effect of the specific inhibitor LY294002 of PI3K/Akt signal transduction pathway on proliferation and expression of PTEN of human tongue squamous carcinoma Tca8113 cell lines cultured in vitro.Methods:The Tca8113 cells were treated with LY294002 at a dose of 0 (the control group),5,10,20,40μmol/L for 24,48,72,96 h.The cell proliferation inhibition rate of the Tca8113 cells were detected by MTT; Immunocytochemical method was used to detect the expression of PTEN of the experiment group 40 μmol/L.

参考文献/References:

[1]Schmid AC, Byrne RD, Vilar R, et a1. Bisperoxovanadium compounds are a potent PTEN inhibitors[J].FEBS Lett,2004, 566(1/3):35
[2]黄文林, 朱孝锋. 信号转导[M]. 北京: 人民卫生出版社, 2005: 219
[3]Li J,Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain , breast and prostate cancer[J]. Science, 1997,275 (5 308):1 876
[4]Rane MJ, Coxon PY, Powell DW, et al.p38 Kinasedependen MAPKAPK2 activation functions as 3phosphoinositidedependent kinase2 for Akt in human neutrophils[J].J Biol Chem,2001,276(5):3 517
[5]Nguyen DM, Chen GA, Reddy R,et al. Potentiation of paclitaxel cytotoxicity in lung and esophageal cancer cells by pharmacologic inhibition of the phosphoinositide3kinase/protein kinase B(Akt)mediated signaling pathway [J].J Thorac Cardiovasc Surg, 2004,127(2):365
[6]张伟峰. PTEN基因的研究进展[J]. 国外医学:外科学分册,2003, 30(5): 294
[7]Squarize CH,Castilho RM,Santos Pinto D Jr.Immunohisto chemical evidence of PTEN in oral squamous cell carcinoma and its correlation with the histological malignancy grading system[J]. J Oral Pathol Med,2002,31(7):379
[8]Lee JI, Soria JC, Hassan KA, et a1.Loss of PTEN expression as a prognostic marker for tongue cancer[J]. Arch Otolaryngol Head Neck Surg,2001,127(12):1 441

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备注/Memo

备注/Memo:
#通讯作者,男,1966年生,博士,主任医师,副教授,研究方向:口腔颌面部肿瘤的防治,Email:xinguanghan@sina.com.cn
更新日期/Last Update: 2010-05-28