[1]高嵩涛)△,邓展生),杜英),等.大鼠去细胞神经支架制备方法的改良[J].郑州大学学报(医学版),2009,(03):616-618.
 GAO Songtao),DENG Zhansheng),DU Ying),et al.Preparation of scaffold of optimized acellular nerve of rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):616-618.
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大鼠去细胞神经支架制备方法的改良 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年03期
页码:
616-618
栏目:
论著
出版日期:
2009-05-01

文章信息/Info

Title:
Preparation of scaffold of optimized acellular nerve of rats
作者:
高嵩涛1)邓展生2)杜英3)张胜利4)郭晓柠5)李宝军2)
1)河南省肿瘤医院骨科郑州4500082)中南大学湘雅医院骨科长沙4100083)郑州大学微生物学与免疫学教研室郑州4500014)重庆市中山医院骨科重庆4080015)中南大学湘雅二医院骨科长沙410083
Author(s):
GAO Songtao1) DENG Zhansheng2) DU Ying3) ZHANG Shengli4) GUO Xiaoning5)etc
关键词:
去细胞神经坐骨神经神经移植大鼠
Keywords:
acellular nervesciatic nervenerve graftrat
分类号:
R651.3;R318.08
摘要:
探讨去除大鼠周围神经中的细胞和髓鞘以取得去细胞神经支架的方法。方法:取15只SD大鼠的坐骨神经30条,随机分为3组(每组10条)。分别为正常神经组(A组),TritonX200处理的化学去细胞组(B组),TritonX200联合sulfobetaine16(SB16)、sulfobetaine10(SB10)去细胞组(C组)。在光镜和电镜下观察各组神经组织的组织学结构,采用免疫组织化学SP法检测各组神经组织中S100蛋白和层黏连蛋白(Laminin)的表达。结果:制备出的去细胞神经呈淡乳白色圆柱状外观,略透亮,其延展性较化学处理前略增加。B、C组神经经去细胞处理后,均去除了Schwann细胞、神经髓鞘和轴突,C组更好地保存了由Schwann细胞基底膜管以及外膜和束膜的细胞外基质构成的三维支架结构。结论:TritonX200联合SB16、SB10改良化学方法处理可获得达到要求的去细胞神经支架。
Abstract:
To explore a method to remove the cells and myelin in peripheral nerve of rats,so as to obtain an acellular nerve scaffold.Methods:30 sciatic nerves from 15 SD rats were divided into three groups randomly(10 nerves in every group). Normal nerves as group A, acellular nerves by chemical disposal of TritonX200 as group B,acellular nerves by chemical disposal of TritonX200, SB10 and SB16 as group C. The characters of the structure were observed under light and electronic microscope, and the components of S100 protein and Laminin protein were analyzed by immunohistochemistry of SP method

参考文献/References:

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相似文献/References:

[1]高嵩涛)△,邓展生)﹟,杜英),等.大鼠脂肪干细胞与不同去细胞神经支架的体外相容性比较[J].郑州大学学报(医学版),2009,(01):132.
 GAO Songtao),DENG Zhansheng),DU Ying),et al.Compatibility between adipose derived stem cells of rats and different acellular nerve scaffold[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):132.

备注/Memo

备注/Memo:
△男,1975年生,博士,主治医师,研究方向:显微外科与骨肿瘤外科,Email:gaost2000@sina.com
更新日期/Last Update: 2010-05-28