[1]崔静,千新来#,李永真,等.钙磷酸蛋白C对乳癌细胞MDAMB435S凋亡的影响*[J].郑州大学学报(医学版),2009,(04):731-734.
 CUI Jing,QIAN Xinlai,LI Yongzhen,et al.Effect of Calphostin C on apoptosis of breast carcinoma cell MDAMB435S[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):731-734.
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钙磷酸蛋白C对乳癌细胞MDAMB435S凋亡的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年04期
页码:
731-734
栏目:
应用研究
出版日期:
2009-07-01

文章信息/Info

Title:
Effect of Calphostin C on apoptosis of breast carcinoma cell MDAMB435S
作者:
崔静千新来#李永真冶亚平李新强贺国洋任峰朱慧芳韩芳毅
新乡医学院病理学教研室 新乡 453003
Author(s):
CUI JingQIAN XinlaiLI YongzhenYE YapingLI XinqiangHE GuoyangREN FengZHU HuifangHAN Fangyi
Department of Pathology, Xinxiang Medical University, Xinxiang 453003
关键词:
钙磷酸蛋白C蛋白激酶C乳癌细胞凋亡MDAMB435S
Keywords:
Calphostin Cprotein kinase Cbreast carcinomaapoptosisMDAMB435S
分类号:
R737.9
摘要:
研究钙磷酸蛋白C对乳癌细胞MDAMB435S凋亡的影响及相关机制。方法:采用倒置显微镜、琼脂糖凝胶电泳和流式细胞术等方法观察钙磷酸蛋白C处理前后MDAMB435S细胞形态、基因组DNA断裂和凋亡细胞数的变化;采用RTPCR检测钙磷酸蛋白C处理前后MDAMB435S细胞凋亡相关基因bclXL、bcl2、IAP1、bax及Smac mRNA表达的变化;采用Western blotting法测定Bax和Bcl2蛋白的表达。结果:与MDAMB435S亲本细胞(对照组)相比,钙磷酸蛋白C处理后的MDAMB435S细胞(实验组)出现凋亡形态学特征(如胞膜发泡、染色质凝集、凋亡小体形成等)改变,显示特征性的DNA“梯状”断裂变化,凋亡细胞数明显增多。RTPCR和Western blotting结果显示:与对照组相比,实验组细胞中促凋亡基因bax和Smac mRNA表达上调,抗凋亡基因bcl2 mRNA表达下调,但抗凋亡基因bclXL和IAP1 mRNA表达没有明显变化;实验组Bax蛋白表达上调,Bcl2蛋白表达下调。结论:钙磷酸蛋白C可诱导MDAMB435S凋亡,该作用可能与上调bax和Smac基因表达及下调bcl2基因表达有关。
Abstract:
To investigate the effect of Calphostin C on the apoptosis of breast carcinoma cell MDAMB435S.Methods:The changes of morphology, DNA cleavage, and number of apoptosis of MDAMB435S cells before and after treated with Calphostin C were observed by inverted microscope, agarose gel electrophoresis, and flow cytometry.The expressions of apoptosisrelated genes bcl2,bclXL,bax,IAP1 and Smac mRNA were detected by RTPCR.Bax and Bcl2 proteins were detected by Western blotting assay.Results: Compared with the parental MDAMB435S cells(control group),morphological changes of apoptosis(blowing up in membrance, chromatic agglutination, and apoptotic bodies,etc), the appearance of “DNA ladder” by agarose gel electrophoresis, and the significant increase of the apoptosis by flow cytometry in the MDAMB435S cells treated with Calphostin C(experimental group) were found.The expressions of bax and Smac mRNA were higher, and the expression of bcl2 mRNA was lower, while the expressions of bclXL and IAP1 mRNA had no significant changes in experinental group. The expression of Bax protein was higher,and that of Bcl2 protein was lower in experimetal group.Conclusion: Calphostin C could induce the apoptosis of MDAMB435S cells, which maybe due to the upregulating expressions of bax and Smac, and the downregulating expression of bcl2.

参考文献/References:

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备注/Memo

备注/Memo:
*卫生部科研基金资助项目WKJ20072022;河南省高校杰出科研人才创新工程资助基金项目2007KYCX013;新乡医学院2006年度高学历人才基金资助项目NO.3#通讯作者,男,1966年生,博士,副教授,研究方向:癌变机制及肿瘤生物治疗,Email:qxlfssws@163.com
更新日期/Last Update: 2010-05-18