[1]陈吉相△,赵婷,黄丹霞,等.凝血酶预处理对大鼠神经细胞凋亡的影响[J].郑州大学学报(医学版),2009,(04):781-783.
 CHEN Jixiang,ZHAO Ting,HUANG Danxia,et al.Effect of thrombin preconditioning of neurocytes apoptosis of rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):781-783.
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凝血酶预处理对大鼠神经细胞凋亡的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年04期
页码:
781-783
栏目:
论著
出版日期:
2009-07-01

文章信息/Info

Title:
Effect of thrombin preconditioning of neurocytes apoptosis of rats
作者:
陈吉相赵婷黄丹霞李慧
华中科技大学同济医学院附属协和医院神经内科 武汉 430022
Author(s):
CHEN Jixiang ZHAO Ting HUANG Danxia LI Hui
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022
关键词:
凝血酶预处理细胞凋亡Caspase3Bcl2
Keywords:
thrombin preconditioningapoptosisCaspase3Bcl2
分类号:
R743
摘要:
观察凝血酶预处理对大鼠神经细胞凋亡的影响。方法:成年雄性SD大鼠50只, 随机分为5组, 每组10只。假手术组(C组):大鼠右侧尾状核注射生理盐水50 μL;小剂量凝血酶组(SDT组):大鼠右侧尾状核注射凝血酶1 U;大剂量凝血酶组(LDT组):大鼠右侧尾状核注射凝血酶10 U;凝血酶预处理组(TL组):大鼠右侧尾状核注射凝血酶1 U,7 d后相同部位注射凝血酶10 U;凝血酶和水蛭素预处理组(THL组):大鼠右侧尾状核注射凝血酶1 U和水蛭素1 U混合液,7 d后相同部位注射凝血酶10 U。5组大鼠均于末次注射后36 h处死取脑,分别行HE和TUNEL染色,RTPCR检测caspase3 和bcl2 mRNA。结果:5组细胞凋亡指数(AI)、caspase2 mRNA及bcl2 mRNA表达量差异有统计学意义(F分别为9.60,9.47,7.49,P均<0.05)。与C组相比,LDT组神经细胞大量凋亡(P<0.01),SDT组没有大量细胞凋亡(P>0.05)。TL组细胞AI较LDT组降低(P<0.01),THL组与LDT组AI差异无统计学意义(P>0.05)。与LDT组相比,TL组caspase3 mRNA表达减少(P<0.01),bcl2 mRNA表达增加(P<0.01)。结论:大剂量凝血酶(10 U)可引起神经细胞凋亡。凝血酶预处理可能通过抑制大剂量凝血酶引起的Caspase3表达增高和Bcl2表达下降发挥抗凋亡作用。

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备注/Memo

备注/Memo:
△男,1962年生,副主任医师,副教授,研究方向:脑血管病、癫痫、运动障碍疾病,Email:cz283895@yahoo.com.cn
更新日期/Last Update: 2010-05-18