[1]刘国红△,杨继要,呼琳,等.胃腺癌及正常胃黏膜组织蛋白质组双向电泳图谱分析[J].郑州大学学报(医学版),2009,(04):786-788.
 LIU Guohong,YANG Jiyao,HU Lin,et al.Analysis of the twodimensional electrophoresis maps of gastric carcinoma and paired normal mucosa[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):786-788.
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胃腺癌及正常胃黏膜组织蛋白质组双向电泳图谱分析 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年04期
页码:
786-788
栏目:
论著
出版日期:
2009-07-01

文章信息/Info

Title:
Analysis of the twodimensional electrophoresis maps of gastric carcinoma and paired normal mucosa
作者:
刘国红杨继要呼琳张钦宪#贾冬音刘璐
郑州大学基础医学院组织学与胚胎学教研室 郑州 450001
Author(s):
LIU GuohongYANG Jiyao HU LinZHANG QinxianJIA DongyinLIU Lu
Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
蛋白质组双向电泳胃癌
Keywords:
proteomicstwodimensional electrophoresisgastric cancer
分类号:
R735.2
摘要:
对胃腺癌与配对正常胃黏膜双向电泳(2DE)图谱进行比较分析,筛选差异表达蛋白质。方法:采用2DE技术分离胃腺癌和正常胃黏膜组织总蛋白,银染后采用magicscan图像获取仪扫描2DE凝胶图像,采用Imagemaster 6.0凝胶图像分析软件进行分析。结果:胃腺癌和正常胃黏膜2DE图谱上蛋白质斑点主要分布在等电点pI 4~7、相对分子质量20 000~90 000范围内,共有28个差异表达蛋白质,其中15个在胃腺癌组织中表达上调,13个表达下调。结论:胃腺癌与正常胃黏膜组织蛋白质组表达有差异。
Abstract:
To analysis the twodimensional electrophoresis(2DE) maps of gastric cancer and paired normal mucosa and to screen differently expressed proteins between them.Methods:2DE was taken to separate the total proteins of gastric cancer and paired normal mucosa.Protein patterns were analyzed by Imagemaster 6.0 software after silverstaining.Results:2DE profiles with high resolution and reproducibility were obtained. 28 protein spots were found as differently expressed proteins,of which 15 upregulated and 13 downregulated in gastric cancer tissue.Conclusion:Gastric cancer expresses proteins differently from paired normal mucosa.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,1953年生,硕士,教授,研究方向:消化道肿瘤分子生物学与转基因动物的建立与应用,Email:qxz53@zzu.edu.cn
更新日期/Last Update: 2010-05-18