[1]罗执芬,周云#,刘明月.紫杉醇联合替尼泊苷对胃癌BGC823细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2009,(05):942-944.
 LUO Zhifen,ZHOU Yun,LIU Mingyue.Effects of taxol and VM26 on proliferation and apoptosis of gastric cancer cell line BGC823[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(05):942-944.
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紫杉醇联合替尼泊苷对胃癌BGC823细胞增殖和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年05期
页码:
942-944
栏目:
应用研究
出版日期:
2009-09-01

文章信息/Info

Title:
Effects of taxol and VM26 on proliferation and apoptosis of gastric cancer cell line BGC823
作者:
罗执芬周云#刘明月
河南省人民医院肿瘤科郑州450003
Author(s):
LUO ZhifenZHOU Yun LIU Mingyue
Department of Oncology, Henan Provincial People’s Hospital,Zhengzhou 450003
关键词:
紫杉醇替尼泊苷胃肿瘤BGC823细胞凋亡
Keywords:
taxolVW26gastric neoplasmBGC823 cellsapoptosis
分类号:
R735.2
摘要:
探讨紫杉醇(Taxol)和替尼泊苷(VM26)联合应用对胃癌BGC823细胞的体外抑制和诱导细胞凋亡的作用。方法:采用MTT法测定Taxol单药(0.75、1.50、3.00、6.00、12.00mg/L),VM26单药(1.25、2.50、5.00、10.00、20.00mg/L)及联合用药(0.75/1.25,1.5/2.50,3.00/5.00,6.00/10.00,12.00/20.00mg/L)对BGC823细胞的生长抑制率,应用流式细胞仪测定0.75mg/LTaxol、1.25mg/LVM26单药及其联合用药作用0,12,24,48h各时间点的细胞周期和凋亡率。结果:Taxol和VM26单药及联合用药对BGC823细胞的生长抑制率随药物的质量浓度的增加而增加。联合用药指数(CI)为1.43。经1.25mg/L的VM26和0.75mg/LTaxol单药及联合用药均可诱导胃癌BGC823细胞的凋亡,Taxol单药处理后24h,使G2/M细胞显著增多,S期细胞显著减少;VM26单药处理后48h,G1/G0、S期细胞减少,G2/M期细胞显著增加。结论:Taxol与VM26单药均可抑制胃癌BGC823细胞的生长,诱导细胞凋亡,联合用药对胃癌BGC823细胞的生长抑制有拮抗作用。
Abstract:
To investigate the effects of taxol and VM26 on proliferation and apoptosis of gastric cancer cell line BGC823.Methods:MTT assay was used to examine the inhibition rate of cell growth when cells were treated with various concentrations of VM26(1.25,2.50,5.00,10.00,20.00 mg/L),taxol(0.75,1.50,3.00,6.00,12.00 mg/L) single and combination for 48 hours; the apoptosis rate was examined by flow cytometry.Results: The inhibition rate of growth was enhanced in a dosedepandent manner when BGC823 cells treated with VM26 single or combined. The value of CI was 1.43. When the cells were treated with 1.25 mg/L VM26,the cell cycle was arrested in the G2/M phase at 48 h.When the cells were treated with 0.75 mg/L taxol,the cell cycle was arrested in the G2/M phase at 24 h.Conclusion:Taxol and VM26 single or combined can inbibit the growth and induce the apoptosis of BGC823 cells.The combination of VM26 and Taxol has antagonism effects on the growth of gastric cancer cell BGC823.

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备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目0424410087 #通讯作者,男,1959年生,硕士,教授,研究方向:肿瘤的基础与临床,Email:zhy1336@yahoo.com.cn
更新日期/Last Update: 2010-05-14