[1]周云)△,王晚萍).人重组p53腺病毒感染p53突变mBGC823细胞对顺铂敏感性的影响*[J].郑州大学学报(医学版),2009,(05):948-950.
 ZHOU Yun),WANG Wanping).Adenovirus mediated p53 gene enhances the sensitivity of gastric cancer cells containing mutanttype p53 to cisplatin[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(05):948-950.
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人重组p53腺病毒感染p53突变mBGC823细胞对顺铂敏感性的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年05期
页码:
948-950
栏目:
应用研究
出版日期:
2009-09-01

文章信息/Info

Title:
Adenovirus mediated p53 gene enhances the sensitivity of gastric cancer cells containing mutanttype p53 to cisplatin
作者:
周云1)王晚萍2)
1)河南省人民医院肿瘤科郑州4500032)长治市人民医院肿瘤科长治046000
Author(s):
ZHOU Yun1)WANG Wanping2)
1)Department of Oncology, Henan Provincial People’s Hospital,Zhengzhou 4500032)Department of Oncology,Changzhi People’s Hospital,Changzhi 046000
关键词:
胃肿瘤顺铂人重组p53腺病毒基因治疗
Keywords:
gastric neoplasmcisplatinhuman recombinant adenovirus p53gene therapy
分类号:
R735.2
摘要:
观察人重组p53腺病毒(rAdp53)感染含突变型p53基因胃癌BGC823细胞(mBGC823)对顺铂(CDDP)敏感性的影响。方法:采用免疫细胞化学和WesternBlot法检测rAdp53(5×1010vp/L)感染mBGC823细胞48h后P53蛋白的表达;MTT法测定rAdp53单药组(5×109、5×1010、5×1011vp/L)、CDDP单药组(3.125、6.25、12.50mg/L)及联合用药组(5×109/3.125、5×1010/6.25、5×1011/12.5vp,mg/L)作用后mBGC823细胞的生长抑制率;流式细胞仪检测其细胞周期与凋亡率。结果:rAdp53感染mBGC823细胞48h,P53蛋白的阳性表达率为(63.74±4.21)%,显著高于对照组的(30.80±5.32)%(t=-13.039,P<0.001);rAdp53、CDDP单药及联合用药均可抑制细胞生长,其作用呈剂量依赖性(P均<0.001)。rAdp53单药产生以G2/M期为主的阻滞,凋亡率为(11.76±2.33)%;CDDP单药产生以G1期为主的阻滞,凋亡率为(24.70±2.42)%;联合用药组产生G2/M期为主阻滞,凋亡率为(51.88±2.03)%;各组凋亡率比较差异有统计学意义(F=1620.595,P均<0.001)。结论:腺病毒介导p53基因感染mBGC823细胞改变了细胞内在突变的p53状态,诱导凋亡并增加对CDDP的敏感性。
Abstract:
To observe the effects of transfection of recombinant adenovirus mediated p53 gene (rAdp53) on gastric cancer cells containing mutanttype p53 (mBGC823) to cisplatin(CDDP).Methods:rAdp53 was transfected into mBGC823. P53 protein expression was detected by immunohistochemistry assay and Western Blot assay.mBGC823 cells were treated with rAdp53(5 ×109,5×1010,5×1011 vp/L),CDDP (3.125,6.25,12.50 mg/L) and combined (5×109/3.125,5×1010/6.25,5×1011/12.5 vp,mg/L). The inhibition rate of mBGC823 cell was examined by MTT assay. Cell cycle distribution and apoptotic rate were determined by flow cytometry.Results: The single use of rAdp53 caused P53 protein upregulation [(63.74±4.21)% vs (30.80±5.32)%](t=-13.039,P<0.001). The proliferation of mBGC823 cells was inhibited in dosedependent manner by rAdp53,CDDP single or combined(P<0.001).Infection of rAdp53 induced G2/M arrest and apoptotic subpeak. After exposed to CDDP alone induced G1 arrest and apoptotic subpeak.Combined administration of rAdp53 and CDDP remarkably arrested the cells in G2/M. Meanwhile the apoptotic rate was (51.88±2.03)% in rAdp53+CDDP group,which was significantly higher than that in rAdp53 group(11.76±2.33)% and CDDP group (24.70±2.42)%(F=1 620.595,P<0.001).Conclusion: Infection of rAdp53 can transfer and restore wildtype p53 gene into the gastric carcinoma cell lines with mutant p53 genetic status. rAdp53 can increase the cellular apoptosis and chemosensitivity of gastric carcinoma cells to CDDP.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省科技重点攻关基金资助项目0624410058 △男,1959年生,硕士,教授,研究方向:肿瘤的基础与临床,Email:zhy1336@yahoo.com.cn
更新日期/Last Update: 2010-05-14