[1]梁红霞,樊哲铭#.氧、糖剥夺预处理对大鼠海马神经元热休克蛋白70、cfos表达的影响[J].郑州大学学报(医学版),2009,(05):1035-1036.
 LIANG Hongxia,FAN Zheming.Expression of HSP70,cfos in rat hippocampal neurons after oxygen and glucose deprivation preconditioning in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(05):1035-1036.
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氧、糖剥夺预处理对大鼠海马神经元热休克蛋白70、cfos表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年05期
页码:
1035-1036
栏目:
论著
出版日期:
2009-09-01

文章信息/Info

Title:
Expression of HSP70,cfos in rat hippocampal neurons after oxygen and glucose deprivation preconditioning in vitro
作者:
梁红霞樊哲铭#
郑州市第五人民医院神经内科郑州450003
Author(s):
LIANG HongxiaFAN Zheming
Department of Neurology,the Fifth People’s Hospital of Zhengzhou,Zhengzhou 450003
关键词:
氧、糖剥夺预处理热休克蛋白70cfos海马神经元大鼠
Keywords:
oxygen and glucose deprivation preconditioningHSP70cfoshippocampal neuronsrat
分类号:
Q189
摘要:
观察氧、糖剥夺预处理对体外培养大鼠海马神经元热休克蛋白70(HSP70)、cfos基因表达的影响,探讨预处理的神经保护机制。方法:取新生SD大鼠海马组织神经元进行原代培养,培养至12d时,随机分为4组:对照组,单纯氧、糖剥夺组,氧、糖剥夺预处理组和致死性氧、糖剥夺组(n=30)。以换用无糖Earle’s液培养作缺糖处理,通入含体积分数为85%N2、含体积分数为10%H2和含体积分数为5%CO2混合气体作缺氧处理,各组均采用相应的处理方法。应用免疫细胞化学SP法检测各组神经元HSP70及cfos的表达。结果:HSP70及cfos的表达在4组比较差异有统计学意义(F=31.76,50.44,P<0.05);氧、糖剥夺预处理组HSP70及cfos的表达较对照组、单纯氧、糖剥夺组和致死性氧、糖剥夺组显著增加。结论:氧、糖剥夺预处理可能通过诱导HSP70及cfos的表达,发挥其神经保护作用。
Abstract:
To observe the expression of HSP70,cfos in rat cortical neuron cultured in vitro after oxygen and glucose deprivation preconditioning.Methods:Hippocampal neurons from newborn rat were cultured and randomly allocated into the following groups:control group,oxygen and glucose deprivation group,oxygen and glucose deprivation preconditioning group,oxygenglucose deprivation causing death group(n=30).The expression of HSP70 and cfos in four groups was measured by using immunocytochemical technique.Results:There were significant differences amomg the 4 groups in the expressions HSP70 and cfos(F=31.76,50.44,P<0.05).The expressions of HSP70 and cfos were markedly increased in oxygen and glucose deprivation preconditioning group.Conclusion:Oxygenglucose deprivation preconditioning has neuroprotective effect,which may be realized by promoting HSP70 and cfos expression.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1981年生,硕士,研究方向:脑缺血预处理,Email:Fanzheming@126.com
更新日期/Last Update: 2010-05-14