[1]吴成富)),杨建萍),陈明勇),等.RNA干扰沉默桩蛋白对食管鳞癌EC9706细胞体外侵袭能力的影响*[J].郑州大学学报(医学版),2009,(06):1130-1134.
 WU Chengfu)),YANG Jianping),CHEN Mingyong),et al.Effects of silencing expression of Paxillin using RNA interference on invasion of esophageal squamous cell carcinoma EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1130-1134.
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RNA干扰沉默桩蛋白对食管鳞癌EC9706细胞体外侵袭能力的影响* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1130-1134
栏目:
食管癌研究
出版日期:
2009-11-01

文章信息/Info

Title:
Effects of silencing expression of Paxillin using RNA interference on invasion of esophageal squamous cell carcinoma EC9706 cells
作者:
吴成富1)2)杨建萍1) 陈明勇3)李晟磊1)陈奎生1)高冬玲1#)
1)郑州大学第一附属医院病理科;河南省肿瘤病理重点实验室 郑州 4500522)信阳市中心医院麻醉科 信阳 4640003)新乡医学院第三附属医院麻醉科 新乡 453000
Author(s):
WU Chengfu1)2)YANG Jianping1)CHEN Mingyong3)LI Shenglei1) CHEN Kuisheng1)GAO Dongling1)
1)Department of Pathology, the First Affiliated Hospital,Zhengzhou University;Henan Key Laboratory of Tumor Pathology, Zhengzhou 4500522)Department of Anesthesia,Xinyang Central Hospital,Xinyang 4640003)Department of Anesthesia,the Third Affiliated Hospital,Xinxiang Medical college,Xinxiang 453000
关键词:
RNA干扰桩蛋白EC9706黏着斑激酶金属基质蛋白酶2
Keywords:
RNA interferencePaxillinEC9706focal adhension kinasematrix metallo proteinase2
分类号:
R735.1
摘要:
探讨RNA干扰(RNAi)沉默桩蛋白(Paxillin)基因表达对人食管鳞癌EC9706细胞系体外侵袭能力的影响。方法:将食管鳞癌EC9706细胞分为正常对照组、转染siRNA对照组以及Paxillin siRNA组,通过RTPCR和Western Blotting方法检测转染48 h后3组细胞Paxillin、FAK和MMP2 mRNA和蛋白的表达;通过侵袭小室实验观察3组细胞体外侵袭能力的变化。结果:3组细胞中Paxillin、FAK和MMP2 mRNA及蛋白的表达水平相比,差异具有统计学意义(mRNA:F=7 021.388、20 147.737和5 992.522,P<0.001;蛋白:F=4 454.293,10 003.335和3 802.389,P<0.001),正常对照组及转染siRNA对照组细胞Paxillin、FAK和MMP2 mRNA及蛋白的表达均高于Paxillin siRNA组(P<0.05);3组穿膜细胞数比较,差异具有统计学意义(F=288.741,P<0.001),正常对照组及转染siRNA对照组的穿膜细胞数高于Paxillin siRNA组(P<0.05)。结论:RNAi沉默Paxillin基因表达可以使EC9706细胞的体外侵袭能力降低。
Abstract:
To study the effect of downregulation of Paxillin gene evoked by RNAi on invasion ability in vitro in esophageal squamous cell carcinoma (ESCC) cell line EC9706.Methods:Cells were divided into three groups: normal control group,control siRNA group and Paxillin siRNA group.Expression of Paxillin,FAX and MMP2 in three groups were analyzed by RTPCR and Western Blotting methods.Changes of invasion ability of EC9706 cells were investigated by Boyden chamber in vitro.Results: Expression level of Paxillin was markedly downregulated in EC9706 cells stably expressing Paxillin RNA, there was significant difference among three groups cells (mRNA:F=7 021.388,20 147.737,and 5 992.522,P<0.001;Protein:4 454.293,10 003.335,and 3 802.389,P<0.001). Besides, the result of Boyden chamber in vitro invasion experiment demonstrated that the number of cells that have migrated to the lower side of the membrane in control group and control siRNA transfection group was obviously higher than that in Paxillin transfection group (F=288.741,P<0.001).Conclusion: Downregulation of Paxillin expression can reduce the invasion ability in EC9706 cells.

参考文献/References:

[1]Das A,Yaqoob U,Mehta D,et al.FXR promotes endothelial cell motility through coordinated regulation of FAK and MMP9[J].Arterioscler Thromb Vasc Biol,2009,29(4):562
[2]Aponte M,Jiang W,Lakkis M,et al.Activation of plateletactivating factor receptor and pleiotropic effects on tyrosine phosphoEGFR/Src/FAK/Paxillin in ovarian cancer [J].Cancer Res,2008,68(14):5 839
[3]van Zyp JV,Conway WC,Craig DH,et al.Extracellular pressure stimulates tumor cell adhesion in vitro by paxillin activation[J].Cancer Biol Ther,2006,5(9):1 169
[4]Schaller MD. FAK and paxillin:regulators of Ncadherin adhesion and inhibitors of cell migration [J].J Cell Biol,2004,166(2):157
[5]Shafikhani SH, Mostov K, Engel J.Focal adhesion components are essential for mammalian cell cytokinesis [J].Cell Cycle,2008,7(18):2 868
[6]Siesser PM,Meenderink LM,Ryzhova L,et al.A FAK/Src chimera with gainoffunction properties promotes formation of large peripheral adhesions associated with dynamic actin assembly [J].Cell Motil Cytoskeleton,2008,65(1):25
[7]WierzbickaPatynowski I, Mao Y,Schwarzbauer JE.Continuous requirement for pp60Src and phosphopaxillin during fibronectin matrix assembly by transformed cells [J].J Cell Physiol, 2007,210(3):750
[8]Jagadeeswaran R,Surawska H,Krishnaswamy S,et al.Paxillin is a target for somatic mutations in lung cancer: implications for cell growth and invasion [J].Cancer Res,2008,68(1):132
[9]Li HG,Xie DR,Shen XM,et al.Clinicopathological significance of expression of paxillin, syndecan1 and EMMPRIN in hepatocellular carcinoma [J].World J Gastroenterol,2005,11(10):1 445
[10]Short SM,Yoder BJ,Tarr SM,et al.The expression of the cytoskeletal focal adhesion protein paxillin in breast cancer correlates with HER2 overexpression and may help predict response to chemotherapy:a retrospective immunohistochemical study [J].Breast J,2007,13(2):130
[11]Schaepfer DD,Mitra SK.Multiple connection s link FAK to cell motility and invasion[J].Curr Opin Genet Der,2004,14(1):92
[12]Yano H,Mazaki Y,Karokawa K,et al. Roles played by subset of integrin signaling molecules in cadherinbased cell cell adhension[J]. J Cell Biol,2004,166(2):283
[13]Chambers AF, Matrisian LM. Changing views of the role of matrix metalloproteinases in metastasis [J]. J Natl Cancer Inst,1997,89(17):1 260
[14]OCharoenrat P,RhysEvans PH,Eccles SA.Expression of matrix metalloproteinases and their inhibitors correlates with invasion and metastasis in squamous cell carcinoma of the head and neck [J]. Arch Otolaryngol Head Neck Surg, 2001,127(7):813
[15]Owens LV,Xu LH,Grven RJ,et al.Overexpression of the focal adhesion kinase(p125FAK) in invasive human tumors [J]. Cancer Res,1995,55(13):2 752
[16]Xie B, Zhao J,Kitagawa M, et al. Focal adhesion kinase activates stat1 in integrinmediated cell migration and adhesion [J]. J Biol Chem, 2001,276 (22):19 512

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备注/Memo

备注/Memo:
*河南省基础与前沿技术研究基金资助082300453202 #通讯作者,女,1964年生,大学本科,主任技师,研究方向:肿瘤病理,Email:gaodlxq@yahoo.com.cn
更新日期/Last Update: 2010-05-14