[1]史祖宣,李克,樊青霞#.食管鳞状细胞癌组织中神经性钙黏附蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(06):1138-1140.
 SHI Zuxuan,LI Ke,FAN Qingxia.Expression of Ncadherin protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1138-1140.
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食管鳞状细胞癌组织中神经性钙黏附蛋白和mRNA的表达* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1138-1140
栏目:
食管癌研究
出版日期:
2009-11-01

文章信息/Info

Title:
Expression of Ncadherin protein and mRNA in esophageal squamous cell carcinoma tissue
作者:
史祖宣李克樊青霞#
郑州大学第一附属医院肿瘤科 郑州 450052
Author(s):
SHI Zuxuan LI Ke FAN Qingxia
Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
食管肿瘤鳞状细胞癌神经性钙黏附蛋白浸润转移
Keywords:
esophageal neoplasmsquamous cell carcinoma Ncadherin invasionmetastasis
分类号:
R735.1
摘要:
检测食管鳞状细胞癌组织中神经性钙黏附蛋白(Ncadherin) 和mRNA的表达。方法:采用免疫组织化学PV法和RTPCR法检测62例食管鳞状细胞癌组织及62例正常食管黏膜组织中Ncadherin蛋白和mRNA的表达。结果:正常食管黏膜组织及食管鳞状细胞癌组织中Ncadherin蛋白的阳性表达率和mRNA的相对含量分别为29.0%、75.8%和(0.154±0.019)、(0.663±0.016),差异均有统计学意义(χ2=23.758,t=1 290.840,P均<0.001)。Ncadherin蛋白阳性表达率及mRNA的相对含量与食管鳞状细胞癌的分化程度、浸润深度及淋巴结转移密切相关(χ2=6.924、9.345、4.486,F/t=555.231、4.971、7.269,P<0.05或0.01)。结论:Ncadherin的高表达与食管鳞状细胞癌的发生发展及浸润转移密切相关。
Abstract:
To explore expression of Ncadherin protein and mRNA in esophageal squamous cell carcinoma (ESCC) tissue.Methods:PV immunohistochemical method and RTPCR were used to detect the expression of Ncadherin protein and mRNA in 62 cases of normal esophageal epithelium and 62 cases of ESCC tissue.Results: The positive expression rate of Ncadherin protein and mRNA expression level in normal esophageal epithelium and ESCC tissue were 29.0%,75.8%,and (0.154±0.019),(0.663±0.016)(χ2=23.758,t=1 290.840,P<0.001). Both protein positive expression rate and mRNA expression level of Ncadherin were related to the differentiation grade,infiltration depth, and lymph node metastasis of ESCC(χ2=6.924,9.345,4.486,F/t=555.231,4.971,7.269,P<0.05 or 0.01).Conclusion: The high expression of Ncadherin is involved in the development, invasion,and metastasis of ESCC.

参考文献/References:

[1]Uchikado Y, Natsugoe S, Okumura H, et al.Slug expression in the Ecadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma[J].Clin Cancer Res, 2005,11(3):1 174
[2]Nair KS,Naidoo R,Chetty R.Microsatellite analysis of the APC gene and immunoexpression of Ecadherin, catenin, and tubulin in esophageal squamous cell carcinoma[J]. Hum Pathol, 2006,37(2):125
[3]Jaggi M,Nazemi T,Abrahams NA,et al.Ncadherin switching occurs in high gleason grade prostate cancer[J].Prostate, 2006,66(2):193
[4]Nagi C,Guttman M,Jaffer S,et al.Ncadherin expression in breast cancer: correlation with an aggressive histologic variantinvasive micropapillary carcinoma[J].Breast Cancer Res Treat, 2005,94(3):225
[5]许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996,6(4):229
[6]Pettitt J.The cadherin superfamily[J].WormBook, 2005:1
[7]Suyama K, Shapiro I, Guttman M,et al. A signaling pathway leading to metastasis is controlled by Ncadherin and the FGF receptor [J].Cancer Cell,2002,2(4):301

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备注/Memo

备注/Memo:
*河南省科技攻关基金资助项目072102310054 #通讯作者,女,1952年生,教授,研究方向:恶性肿瘤的综合防治,Email:fqx2243@yahoo.com.cn
更新日期/Last Update: 2010-05-14