[1]臧文巧),杨璇),王涛),等.豫医无毛小鼠无毛基因突变部位的定位和分析*[J].郑州大学学报(医学版),2009,(06):1144-1145.
 ZANG Wenqiao),YANG Xuan),WANG Tao),et al.Orientation and analysis of Yuyi hairless mice hairless gene mutation point[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1144-1145.
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豫医无毛小鼠无毛基因突变部位的定位和分析*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1144-1145
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Orientation and analysis of Yuyi hairless mice hairless gene mutation point
作者:
臧文巧1) 杨璇1)王涛2)轩小燕1)李敏1)杜英1)#
1)郑州大学基础医学院微生物学与免疫学教研室 郑州 4500012)河南中医学院第一附属医院血液肿瘤科 郑州 450000
Author(s):
ZANG Wenqiao1)YANG Xuan1)WANG Tao2)XUAN Xiaoyan1)LI Min1)DU Ying1)
1)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 4500012)Department of Hematotumor,the First Affiliated Hospital,College of Traditional Chinese Medical Sciences of Henan Province, Zhengzhou 450000
关键词:
豫医无毛小鼠基因突变
Keywords:
Yuyi hairless mice gene mutation
分类号:
R394.33
摘要:
研究豫医无毛小鼠(YYHL)突变的分子机制。方法:对豫医无毛小鼠和昆明小鼠的基因组DNA进行PCR扩增,对扩增片段克隆后测序,并与Mus muscnlus进行同源性分析。结果:共测出YYHL DNA序列1 824 bp和昆明小鼠DNA序列1 816 bp,其不同之处有27处,变异方式包括插入、缺失和点突变。其中在12外显子中有一无义突变(G→A),导致该部位由编码色氨酸的UGG转变为终止密码子UGA。结论:YYHL无毛性状的产生可能与基因组的变异有关。
Abstract:
To research the hairless gene mutation of Yuyi hairless mice.Methods: Partial hairless gene of Yuyi hairless mice and Kunming mice were cloned, sequenced,and compared with Mus musculus DNA.Results: The DNA fragment of Yuyi hairless mice contained 1 824 bp,that of Kunming mice contained 1 816 bp.There were 27 differences, including insertion, deletion,and point mutation. One nonsense mutation in exon 12 resulted in the introduction of a premature termination codon UGA.Conclusion:Hairless character is probably associated with the hairless gene mutation of Yuyi hairless mice.

参考文献/References:

[1]轩小燕,付庆,杜献堂,等. 豫医无毛小鼠无毛基因DNA原位PCR检测[J].郑州大学学报:医学版,2003,38(2):203
[2]Kim H, Panteleyev AA, Jahoda CA. Genomic organization and analysis of the hairless gene in four hypotrichotic rat strains [J].Mamm Genome, 2004,15(12):975
[3]Zarach JM,Beaudoin GM 3rd,Coulombe PA,et al. The corepressor hairless has a role in epithelial cell differentiation in the skin [J].Development, 2004,131(17):4 189
[4]Martynova MIu, Isaev DA, Koniukhov BV. Analysis of the effects of mutant hairless genes in chimeric mice [J]. Genetika,2003,39(9):1 252
[5]Sieger D, Tautz D, Gajewski M. The role of suppressor of hairless in notch mediated signalling during zebrafish somitogenesis[J]. Mech Dev, 2003,120(9):1 083

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目39970119 #通讯作者,女,1962年生,教授,研究方向:自身免疫病,Email:duying@zzu.edu.cn
更新日期/Last Update: 2010-05-14