[1]沈燕)△,李进香),王喜英),等.无症状HIV感染者HIV共受体及其配体趋化因子的检测*[J].郑州大学学报(医学版),2009,(06):1156-1158.
 SHEN Yan),LI Jinxiang),WANG Xiying),et al.Detection of HIV coreceptors and their ligands in HIVinfected individuals with no clinical symptom of HIV disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1156-1158.
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无症状HIV感染者HIV共受体及其配体趋化因子的检测* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1156-1158
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Detection of HIV coreceptors and their ligands in HIVinfected individuals with no clinical symptom of HIV disease
作者:
沈燕1)李进香2)王喜英1)徐翠英1)
1)郑州大学第一附属医院检验科 郑州 4500522)郑州市儿童医院检验科 郑州 450052
Author(s):
SHEN Yan1) LI Jinxiang2) WANG Xiying1) XU Cuiying1)
1)Department of Clinical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500522)Department of Clinical Laboratory, Zhengzhou Children Hospital, Zhengzhou 450052
关键词:
HIV感染趋化因子HIV共受体
Keywords:
HIV infection chemokine HIV coreceptor
分类号:
R446.6
摘要:
检测无症状HIV感染者HIV共受体CCR5、CXCR4在T淋巴细胞表面的表达以及CCR5、CXCR4的配体MIP1α、RANTES和SDF1在血清中的水平。方法:设立无症状HIV感染组(n=50)和HIV未感染对照组(n=40),采用ELISA方法检测血清中趋化因子MIP1α、RANTES和SDF1水平,流式细胞术分析CCR5、CXCR4在T淋巴细胞胞表面的表达情况。结果:与对照组相比,感染组血清中MIP1α和RANTES水平升高(t分别为2.225、2.706,P<0.05),SDF1水平无变化(t=1.832,P>0.05)。感染组CD4+和CD8+T细胞表面CCR5的表达高于对照组(t分别为11.740、2.157,P<0.05),而感染组CD4+和CD8+T细胞表面CXCR4的表达下降(t分别为2.689、2.756,P<0.05)。感染组MIP1α、RANTES、SDF1、CD4+CCR5+T细胞数、CD4+CXCR4+T细胞数、CD8+CCR5+T细胞数及CD8+CXCR4+T细胞数间无相关关系(P>0.05)。结论:无症状HIV感染者HIV共受体的表达及其配体趋化因子水平均发生明显改变,2者的变化未呈明显的关联性。
Abstract:
To detect the serum levels of MIP1α,RANTES,SDF1 as well as the expression of CCR5/CXCR4 on CD4+/CD8+ T lymphocytes in HIVinfected individuals with no clinical symptom.Methods:A total of 50 HIV infection patients with no clinical symptom of HIV disease (named as the infection group) and 40 HIV uninfection person (called as the control group) were collected. ELISA was used to detect the levels of the investigated chemokines.The expression of CCR5/CXCR4 was analyzed by flow cytometry.Results: Compared with the control group, the levels of MIP1α and RANTES increased(t was 2.225 and 2.706,P<0.05). There was no significant difference between the infection group and the control group on the serum level of SDF1(t=1.832,P>0.05). CD4+CCR5+ and CD8+CCR5+T cells increased in individuals of the infection group than those of the control group (t was 11.740 and 2.157,P<0.05),while CD4+CXCR4+ and CD8+CXCR4+T cells reduced in the infection group(t was 2.689 and 2.756,P<0.05).There was no relationship among MIP1α,RANTES,SDF1,CD4+CCR5+T cells,CD4+CXCR4+T cells,CD8+CCR5+T cells and CD8+CXCR4+T cells in the infection group(P>0.05).Conclusion:The levels of the HIVcoreceptors and their associated chemokines change greatly in HIVinfected individual with no clinical symptom of HIV disease. There is no significant relationship between the HIVcoreceptors and their associated chemokines.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省艾滋病科技攻关计划基金资助项目200620;河南省教育厅自然科学基金资助项目2006320017△女,1966年生,博士,副教授,研究方向:免疫分子的诊断与临床,Email:shenyan_1966@hotmail.com
更新日期/Last Update: 2010-05-14