[1]孟建波)△,康胜群),康素娴),等.骨髓干细胞移植治疗糖尿病兔下肢缺血效果观察*[J].郑州大学学报(医学版),2009,(06):1168-1170.
 MENG Jianbo),KANG Shengqun),KANG Suxian),et al.Effect of transplantation of autologous bone marrow stem cell on diabetic lowe limb ischemia of rabbits[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1168-1170.
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骨髓干细胞移植治疗糖尿病兔下肢缺血效果观察*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1168-1170
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Effect of transplantation of autologous bone marrow stem cell on diabetic lowe limb ischemia of rabbits
作者:
孟建波1)康胜群2)康素娴1)杨彦1)周晨光1)
1)河北医科大学第三医院血液内科 石家庄 0500512)河北医科大学第三医院内分泌科 石家庄 050051
Author(s):
MENG Jianbo1) KANG Shengqun2)KANG Suxian1)YANG Yan1)ZHOU Chenguang1)
1)Department of Hematology,the Third Affiliated Hospital,Hebei Medical University,Shijiazhuang 0500512)Department of Endocrinology,the Third Affiliated Hospital,Hebei Medical University,Shijiazhuang 050051
关键词:
粒细胞集落刺激因子骨髓干细胞移植糖尿病下肢缺血性病变
Keywords:
granulocyte colony stimulating factorbone marrow stem cell transplantationdiabetelower limb ischemiarabbit
分类号:
R587.2;R457.7
摘要:
对比骨髓干细胞移植、粒细胞集落刺激因子(GCSF)动员及局部骨髓干细胞移植联合GCSF动员3种方法对糖尿病兔缺血组织血管新生的作用。方法:40只健康新西兰兔,用四氧嘧啶(ALX)诱导成为糖尿病兔后,均结扎右后肢股动脉,建立糖尿病下肢缺血动物模型,然后随机分为4组,每组10只。其中1组不进行治疗作对照(A组),余3组分别用GCSF动员(B组)、局部骨髓干细胞移植(C组)及GCSF动员联合局部骨髓干细胞移植(D组)进行治疗。28 d后均以右侧股内侧正中部位为中心等距离取局部肌肉组织,免疫组织化学染色检测肌组织微血管密度及血管内皮生长因子(VEGF)表达水平。结果:上述A、B、C、D 4组缺血组织微血管密度(1)分别为(22.0±6.0)、(35.8±7.5)、(38.1±7.2)和(48.7±9.1)个/高倍视野, VEGF蛋白表达水平(2)分别为(8.87±1.51)%、(11.83±1.65)%、(12.32±1.34)%和(17.13±2.73)%,局部干细胞移植(1)和GCSF动员(2)对微血管密度和VEGF的表达有影响(F1分别为50.599、35.930,F2分别为39.846,25.435,P均<0.001,但F1×2分别为2.256,0.437,P>0.05)。结论:GCSF动员和局部骨髓干细胞移植均可用于治疗糖尿病下肢缺血性疾病。
Abstract:
To compare the effects of three methods, which were bone marrow stem cell(BMSCs) transplantation, granulocyte colony stimulating factor(GCSF) mobilization, and transplantation combined with mobilization, on lower limb ischemia of diabetic rabbit.Methods:Rabbits were induced to diabetic model by alloxan and ligatured arteria femoralis of the right side posterior limb, then divided into 4 groups randomly. Group A was the model group. Group B were treated with GCSF mobilization. Group C were treated with BMSCs transplantation. Group D were treated with BMSCs transplantation combined with GCSF mobilization.28 days later, rabbits of the 4 groups were executed. Partial musculatures were stained by the method of immunohistochemistry to detect the microvessel density and VEGF.Results:In group A,B,C,and D, microvessel density was (22.0±6.0),(35.8±7.5),(38.1±7.2), and (48.7±9.1) piece/sight,respectively, and the expression of VEGF was (8.87±1.51)%,(11.83±1.65)%,(12.32±1.34)%,and (17.13±2.73)%,respectively.BMSCs transplantation(1) and GCSF mobilization(2) affected the microvessel density and the expression of VEGF(F1 was 50.599,35.930,F2 was 39.846,25.435,respectively,P<0.001,but F1×2was 2.256,0.437,respectively,P>0.05).Conclusion: Both BMSCs transplantation and GCSF mobilization may be useful for diabetic lower limb ischemia treatment.

参考文献/References:

[1]陆德宾,陈兵,梁自文,等.自体骨髓间充质干细胞移植治疗糖尿病足1例[J].第三军医大学学报,2007,29(11):125
[2]Subramaniyam V, Waller EK, Murrow JR,et al. Bone marrow mobilization with granulocyte macrophage colonystimulating factor improves endothelial dysfunction and exercise capacity in patients with peripheral arterial disease[J].Am Heart J,2009,158(1):53
[3]Wang QR, Wang F, Zhu WB, et al.GMCSF accelerates proliferation of endothelial progenitor cells from murine bone marrow mononuclear cells in vitro[J].Cytokine,2009,45(3):174
[4]Rehman J, Li J, Orschell CM, et al.Peripheral blood “endothelial progenitor cells” are derived from monocyte/macrophages and secrete angiogenic growth factors[J].Circulation,2003,107(8):1 164
[5]Annabi B, Lee Y, Turcotte S, et al.Hypoxia promotes murine bone marrow derived stromal cell migration and tube formation[J].Stem Cells, 2003,21(3):337
[6]Serefhanoglu S, Goker H, Buyukasik Y,et al.Changes in vascular endothelial growth factor, angiopoietins, and Tie2 levels with GCSF stimulation in healthy donors[J].Ann Hematol,2009,88(7):667
[7]Tepper OM,Capla JM,Gallano RD, et al.Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone marrowderived cells[J].Blood, 2005,105(3):1 068
[8]Sbaa E,Dewever J, Martinive P, et al.Caveolin plays a central role in endothelial progenitor cell mobilization and homing in SDF1 driven postischemic vasculogenesis[J].Circ Res,2006,98(9):1 219

备注/Memo

备注/Memo:
△男,1972年生,副教授,副主任医师,研究方向:血液病及外周缺血性疾病的干细胞移植治疗,Email:mjb1972@tom.com *河北省科技厅重大疾病及创新药物专项基金资助项目06276106D
更新日期/Last Update: 2010-05-14