[1]李冀,沈海娥,布仁满都呼,等.蓝氏贾第鞭毛虫染色体的制备*[J].郑州大学学报(医学版),2009,(06):1182-1184.
 LI Ji,SHEN Haie,Burenmanduhu,et al.Chromosome preparation of Giardia lamblia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1182-1184.
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蓝氏贾第鞭毛虫染色体的制备*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1182-1184
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Chromosome preparation of Giardia lamblia
作者:
李冀沈海娥布仁满都呼高青田喜凤#
华北煤炭医学院生物科学系 唐山 063000
Author(s):
LI JiSHEN Hai’eBurenmanduhuGAO QingTIAN Xifeng
Department of Biological Sciences, North China Coal Medical University,Tangshan 063000
关键词:
蓝氏贾第鞭毛虫染色体制备
Keywords:
Giardia lambliachromosomepreparation
分类号:
Q782
摘要:
探讨蓝氏贾第鞭毛虫(简称贾第虫)在分裂过程中是否形成典型的凝集染色体以及染色体的数目。方法:用改良的TYIS33培养基培养贾第虫滋养体。分别在不同浓度的秋水仙素(0、0.05、0.10、0.20、0.40和0.80 mg/L)、低渗处理不同时间(30、50、55、65和80 min)和不同固定液[V(甲醇)V(冰醋酸)为31和21]等条件下制备贾第虫染色体。结果和结论:在制备贾第虫染色体时,秋水仙素的最佳作用浓度为0.20 mg/L,最佳低渗处理时间为55 min,固定液推荐V(甲醇)V(冰醋酸)为31。贾第虫在分裂过程中形成典型的凝集染色体,染色体数目为2n=10。
Abstract:
To study whether the Giardia lamblia have typical chromosomes and the chromosome number during mitosis.Methods:Trophozoites of Giardia lamblia were grown in modified TYIS33 medium at 37 ℃.The medium was removed and cell cultures were enriched with fresh, warmed medium.The chromosome preparation were reseached from content of colchicines(0,0.05,0.10,0.20,0.40,and 0.80 mg/L),time of low osmosis(30,50,55,65, and 80 min) and the kind of fixation fluid[V(carbinol)V(glacial acetic acid) was 31 or 21].Results and Conclusion:The prior colchicine concentration,low osmosis and the fixation fluid were 0.20 mg/L, 55 min and V(carbinol)V(glacial acetic acid)=31.The Giardia lamblia have typical chromosomes during mitosis, and the chromosome number of Giardia lamblia is 2n=10.

参考文献/References:

[1]Adam RD.Biology of Giardia lamblia[J]. Clin Microbiol Rev,2001,14(3) :447
[2]沈剑钊,李靖炎,卢思奇.源真核生物蓝氏贾第虫核分型的初步研究[J].动物学研究,1996,17(3):295
[3]Sagolla MS,Dawson SC,Mancuso JJ,et al. Threedimensional analysis of mitosis and cytokinesis in the binucleate parasite Giardia intestinalis[J].J Cell Sci,2006,119(23):4 889
[4]Adam RD,Nash TE,Wellems TE. The Giardia lamblia trophozoite contains sets of closely related chromosomes[J].Nucleic Acids Res,1988,16(10):4 555
[5]卢思奇,王正仪,视虹.贾第虫纯培养的建立[J].中国寄生虫学与寄生虫病杂志,1990,8(3):199

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备注/Memo

备注/Memo:
*河北省科技厅科技支撑计划项目07276101D68 #通讯作者,女,1957年生,教授,研究方向:病原微生物
更新日期/Last Update: 2010-05-14