[1]朱红灿)△,卢欣),赵鹏),等.促红细胞生成素对体外培养的SD大鼠多巴胺能神经元细胞活力的影响*[J].郑州大学学报(医学版),2009,(06):1185-1187.
 ZHU Hongcan),LU Xin),ZHAO Peng),et al.Effects of erythropoietin on viability of dopaminergic neurons of SD rats in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1185-1187.
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促红细胞生成素对体外培养的SD大鼠多巴胺能神经元细胞活力的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1185-1187
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Effects of erythropoietin on viability of dopaminergic neurons of SD rats in vitro
作者:
朱红灿1)卢欣1)赵鹏1)臧卫东2)张华2)任秀花2)张博爱1)
1)郑州大学第一附属医院神经内科 郑州 4500522)郑州大学基础医学院人体解剖学教研室 郑州 450001
Author(s):
ZHU Hongcan1)LU Xin1)ZHAO Peng1)ZANG Weidong2)ZHANG Hua2)REN Xiuhua2)ZHANG Bo’ai1)
1)Department of Neurology, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
促红细胞生成素多巴胺能神经元细胞活力帕金森病SD大鼠体外
Keywords:
erythropoietindopaminergic neuroncell viabilityParkinson's diseaseSD ratin vitro
分类号:
R742.5
摘要:
探讨促红细胞生成素(EPO)对体外培养的SD大鼠帕金森病细胞模型多巴胺能神经元细胞活力的影响。方法:取孕14.5 d的SD大鼠胚胎中脑原代培养。将细胞分成:正常对照组、6羟基多巴(6OHDA)组和6OHDA+不同剂量EPO(0.10、1.00、3.25、10.00和32.50 U)组。在培养的第6天给实验组加入不同剂量的EPO,第8天加入6OHDA(100 μmol/L)作用0.5 h,收集各组细胞,用酪氨酸羟化酶(TH)免疫组织化学染色法观察TH阳性神经元生长情况,用MTT法测定多巴胺能神经元的细胞活力。结果:与正常对照组相比,6OHDA组TH阳性细胞数减少,为(27.2±3.4)个/mm2,而预先给予EPO干预的各组细胞6OHDA的毒性作用明显减轻,TH阳性细胞数分别为(28.0±1.1)、(39.2±1.9)、(41.8±1.3)、(66.4±4.2)和(65.3±3.0)(F=48.29,P=0.02)个/mm2。与正常对照组相比,6OHDA组多巴胺能神经元的细胞活力为(51.2±1.2)%,而预先给予EPO干预的各组细胞6OHDA的毒性作用明显减轻,细胞活力分别为(52.2±1.0)%、(60.5±3.5)%、(68.1±2.7)%、(89.6±3.1)%和(76.5±4.0)%(F=28.42,P=0.01)。结论:EPO能够对抗6OHDA的毒性作用,增加多巴胺能神经元的细胞活力。
Abstract:
To study the effects of erythropoietin (EPO) on viability of dopaminergic neurons in Parkinson's disease (PD) model in vitro.Methods:Cultured mesencephalic neurons were prepared from embryonic 14.5 d SD rats.The dopaminergic neurons were categorized into seven groups: control, 6OHDA, and 6OHDA+EPO(0.10,1.00,3.25,10.00,32.50 U).Dopaminergic neuron cells were treated with or without various dose of EPO (0.10,1.00,3.25,10.00,32.5 U)on 6th day of culture. On 8th day of culture, the cells were cotreated with the toxin 6OHDA (100 μmol/L) for 0.5 h, and then were collected.Tyrosine hydroxylase (TH) immunohistochemical method was used to observe the growth of TH positive neurons, and the cell viability was evaluated by MTT assay.Results: There were significant differences between 6OHDA group and control.The number of TH positive neurons was (27.2±3.4), and the cell viability was (51.2±1.2)% in 6OHDA group; while the number of TH positive neurons and the viability of neurons increased in the 6OHDA+EPO (0.10,1.00,3.25,10.00,32.50 U) groups.The number of TH positive neurons were (28.0±1.1),(39.2±1.9),(41.8±1.3),(66.4±4.2), and (65.3±3.0) respetively(F=48.29,P=0.02),and the cell viability were (52.2±1.0)%,(60.5±3.5)%,(68.1±2.7)%,(89.6±3.1)%,and (76.5±4.0)% respetively(F=28.42,P=0.01).Conclusion: EPO could increase cell viability and has protective effects on dopaminergic neurons of SD rats in vitro.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省自然科学基金资助项目511040100 △男,1966年生,博士,副主任医师,副教授,研究方向:帕金森病,Email:zhc660407@hotmail.com
更新日期/Last Update: 2010-05-14