[1]贺显君)),索爱琴)#,许予明),等.Aβ142诱导小胶质细胞的炎性上清液对大鼠神经细胞凋亡及Caspase3、PARP蛋白表达的影响[J].郑州大学学报(医学版),2009,(06):1191-1193.
 HE Xianjun)),SUO Aiqin),XU Yuming),et al.Effects of Aβ142induced microglial inflammatory supernatant on rat neuronal apoptosis and expression of Caspase3 and PARP[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1191-1193.
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Aβ142诱导小胶质细胞的炎性上清液对大鼠神经细胞凋亡及Caspase3、PARP蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1191-1193
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Effects of Aβ142induced microglial inflammatory supernatant on rat neuronal apoptosis and expression of Caspase3 and PARP
作者:
贺显君1)2)索爱琴3)许予明1)张杰文3)李玮3)
1)郑州大学第一附属医院神经内科 郑州 4500522)商丘市第一人民医院神经科 商丘 4760003)河南省人民医院神经内科 郑州 450003
Author(s):
HE Xianjun1)2)SUO Aiqin3) XU Yuming1) ZHANG Jiewen3)LI Wei3)
1)Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Neurology, the First People’s Hospital of Shangqiu, Shangqiu 476000 3)Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003
关键词:
Aβ142小胶质细胞阿尔茨海默病Caspase3聚腺苷酸二磷酸核糖转移酶大鼠
Keywords:
Aβ142microgliaAlzheimer's diseaseCaspase3PARPrat
分类号:
R742
摘要:
探讨Aβ142诱导小胶质细胞的炎性上清液对大鼠神经细胞凋亡及Caspase3、聚腺苷酸二磷酸核糖转移酶(PARP)蛋白表达的影响。方法:取生长良好的1940小胶质细胞与终浓度为125 nmol/L的Aβ142共孵育4 h, 取上清液。将培养7 d的大鼠神经细胞分为3组,A组加入炎性上清液,B组加入Aβ142,C组为空白对照组,不做任何处理,分别培养12、24、48与72 h。运用吖啶橙荧光染色法观察神经细胞的凋亡,应用比色试剂盒检测Caspase3的活性,并采用Western Blot检测A组培养不同时间PARP的表达情况。结果:终浓度125 nmol/L的Aβ142诱导的小胶质细胞炎性上清液能够诱导神经细胞凋亡;培养48 h及72 h后,3组细胞Caspase3活性比较差异均有统计学意义(F组间=22.203,P=0.042,F时间=19.883,P=0.048),其中A组培养48 h及72 h后Caspase3活性较B、C组升高(P均<0.05);A组可检测到PARP降解。结论:Aβ142诱导小胶质细胞的炎性上清液可以通过活化Caspase3,降解其底物诱发神经元凋亡。
Abstract:
To investigate the effects of Aβ142induced microglial inflammatory supernatant on rat neuronal apoptosis and expression of Caspase3 and PARP.Methods:The 1940 microglia was mixed with 125 nmol/L Aβ142and cultivated for 4 h,and then collected the supernatant. The rat neurons cultivated for 7 days were allocated into 3 groups. Group A and group B were added microglial inflammatory supernatant and Aβ142,respectively,and group C was normal control group.The cells in 3 groups were cultivated for 12 h,24 h,48 h, and 72 h,respectively. The acridine orange fluorometric method was applied to observe the cell apoptosis. Caspase3 activity was detected with shade selection kit. Western Blot was used to detect PARP degradation of group A at different time points.Results: Microglial inflammatory supernatant induced by 125 nmol/L Aβ142could induce neuron apoptosis. After 48 h or 72 h, the expression of Caspase3 among 3 groups had significant difference (Fgroup=22.203,P=0.042,Ftime=19.883,P=0.048),and the expression of Caspase3 in group A was stronger than those of group B and C(P<0.05).The degradation of PARP could be detected in group A. Conclusion: Aβ142 induced microglial inflammatory supernatant can induce rat neuron apoptosis by activating Caspase3 and degrading its substrate PARP.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,女,1946年生,大学本科,主任医师,教授,研究方向:神经系统变性疾病
更新日期/Last Update: 2010-05-14