[1]潘胜军)△,李丽蓉),王雨若).猫背外侧脑桥中脑被盖区神经细胞损毁对睡眠觉醒状态的影响[J].郑州大学学报(医学版),2009,(06):1215-1217.
 PAN Shengjun),LI Lirong),WANG Yuruo).Effects of damage in cat dorsolateral pontomesencephalic tegmentum on sleepwaking states[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1215-1217.
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猫背外侧脑桥中脑被盖区神经细胞损毁对睡眠觉醒状态的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1215-1217
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Effects of damage in cat dorsolateral pontomesencephalic tegmentum on sleepwaking states
作者:
潘胜军1)李丽蓉1)王雨若2)
1)黄淮学院护理系 驻马店 4630002)郑州大学基础医学院生理学教研室 郑州 450001
Author(s):
PAN Shengjun1)LI Lirong1)WANG Yuruo2)
1)Department of Nursing, Huanghuai University, Zhumadian 4630002)Department of Physiology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
睡眠觉醒状态快眼动睡眠背外侧脑桥中脑被盖区肌迟缓红藻氨酸
Keywords:
sleepwaking statesrapid eye movement sleep dorsolateral pontomesencephalic tegmentum muscle atonia kainic acidcat
分类号:
R338
摘要:
损毁猫背外侧脑桥中脑被盖区神经细胞,观察其对睡眠觉醒状态的影响。方法:采用红藻氨酸(KA)微量注射入猫(6只)双侧背外侧脑桥中脑被盖区(每侧6 μg),损毁该区域。通过脑电活动和肌电活动测量损毁前后动物的觉醒睡眠状态,统计损毁前后各时相数据占每次睡眠描记时间的百分比、每次睡眠描记的快眼动(REM)睡眠次数及每次REM睡眠时间。结果:损毁后觉醒时间的百分比从损毁前的(14.68±1.37)%增加到(20.78±3.37)%(t=-1.350,P=0.235); 慢波睡眠时间从(65.07±2.73)%减少到(64.65±4.35)%(t=0.089,P=0.932);REM睡眠时间从(20.26±2.74)%减少到(13.65±2.25)%(t=3.843, P=0.012); REM睡眠次数从损毁前的(12.2±1.6)次/6 h减少到(10.0±1.4)次/6 h(t=1.976,P=0.119); REM睡眠时间从(6.3±0.6) min/次减少到(5.5±0.5) min/次(t=0.939,P=0.401)。有4只动物在双侧背外侧脑桥中脑被盖区神经细胞损毁后的REM睡眠过程中仍维持一定的肌张力,而在正常睡眠描记过程中肌张力完全消失,只出现时相性的肌肉颤搐。损毁部位的组织学观察显示距损毁部位1~2 mm内的神经细胞消失,损毁区域的神经纤维破坏不明显。结论:背外侧脑桥中脑被盖区的神经细胞在REM睡眠的维持及其伴随的颈肌迟缓的产生中起重要作用。
Abstract:
To investigate the effects of damage in cat dorsolateral pontomesencephalic tegmentum on sleepwaking states.Methods:Kainic acid(KA) were injected bilaterally (6 μg each side) into the dorsolateral pontomesencephalic tegmentum to destroy these areas in 6 cats. Sleepwaking states were measured by electroencephalogram and electromyogram before and after KA injection to compare the percentage changes of every state in each recording time, changes in REM times and REM duration.Results:The percentage of the waking time increased from (14.68±1.37)% before the lesions to (20.78±3.37)%(t=-1.350,P=0.235) after the lesions; slow wave sleep time decreased from (65.07±2.73)% to (64.65±4.35)% (t=0.089,P=0.932); REM sleep time decreased from (20.26±2.74)% to (13.65±2.25)% (t=3.843, P=0.012). The times of REM sleep episode decreased from (12.2±1.6)/6 h before to (10.0±1.4)/6 h (t=1.976,P=0.119)after the lesions; the duration per episode of REM sleep deceased from (6.3±0.6)min to (5.5±0.5) min (t=0.939,P=0.401). In 4 cats, the muscle tension was still kept during REM after neurons lesions in the bilateral dorsolateral pontomesencephalic tegmentum, which was totally absent during the normal REM sleep and showed phasic muscle twitches only. Histological stain in the lesion areas result showed that the neurons disappeared within 1~2 mm area around the KA injection site, whereas the neuronal fibers kept intact. Conclusion:These results suggest that the dorsolateral pontomesencephalic tegmental neurons are critically involved in the maintenance of REM sleep and its muscle atonia events.

参考文献/References:

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备注/Memo

备注/Memo:
△男,1962年生,博士,讲师,研究方向:睡眠机制、血压调节,Email:sjp680@126.com
更新日期/Last Update: 2010-05-14