[1]李留霞),王甜甜),王凯娟),等.六种肿瘤相关抗原自身抗体微阵列诊断卵巢癌的价值[J].郑州大学学报(医学版),2009,(06):1235-1237.
 LI Liuxia),WANG Tiantian),WANG Kaijuan),et al.Value of detecting autoantibodies to six tumorassociated antigens miniarray for ovarian cancer diagnosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1235-1237.
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六种肿瘤相关抗原自身抗体微阵列诊断卵巢癌的价值()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1235-1237
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Value of detecting autoantibodies to six tumorassociated antigens miniarray for ovarian cancer diagnosis
作者:
李留霞1)王甜甜1)王凯娟2)代丽萍2)张建营2)#
1)郑州大学第一附属医院妇产科 郑州 4500522)郑州大学公共卫生学院,郑州大学蛋白质组学应用研究中心 郑州 450052
Author(s):
LI Liuxia1) WANG Tiantian1)WANG Kaijuan2) DAI Liping2)ZHANG Jianying2)
1)Department of Gynaecology and Obstetrics, the First Affliliated Hospital, Zhengzhou University, Zhengzhou 4500522)College of Public Health;Proteomics Research Center,Zhengzhou University,Zhengzhou 450052
关键词:
卵巢癌肿瘤相关抗原自身抗体
Keywords:
ovarian cancertumorassociated antigenautoantibody
分类号:
R737.31
摘要:
检测卵巢肿瘤患者血清中Survivin、P53、P16、CyclinB1、CyclinD1和CyclinE 6种肿瘤相关抗原(TAAs)自身抗体,评价TAAs微阵列对卵巢癌诊断及术后病情监测的价值。方法:用间接ELISA法检测55例原发性上皮性卵巢癌、40例卵巢良性肿瘤、35例正常对照及23例卵巢癌患者术后化疗期间69份血清中以上6种TAAs自身抗体。结果:检测1种TAAs抗体诊断卵巢癌的阳性率为9.1%~23.6%,Survivin最高,CyclinE最低;随TAAs联合种类由1种增加至6种,诊断卵巢癌的灵敏度增加至63.6%,特异度达85.7%,阳性和阴性预测值及约登指数分别为87.5%、60.0%及0.493。6种TAAs微阵列检测阳性率在不同组织学类型和临床分期卵巢癌组织间的差异均无统计学意义(χ2分别为0.622、0.135,P均>0.05),而卵巢癌患者术后化疗各组阳性率(8.7%~34.8%)有较术前(65.2%)降低的趋势。结论:联合6种TAAs微阵列检测血清自身抗体对卵巢癌诊断和术后病情监测可能具有重要参考价值。
Abstract:
To analyze the expression of autoantibodies to six tumorassociated antigens(TAAs) in sera of ovarian tumor patients,including Survivin, P53, P16, CyclinB1, CyclinD1,and CyclinE, and to evaluate the value of TAAs miniarray in diagnosis and state monitoring after operation of ovarian cancer.Methods:A total of 55 primary epithelial ovarian cancer patients, 40 ovarian benign tumor patients,35 normal contol, and 23 patients of ovarian cancer during chemotherapy after operation were subjected to detect the 6 TAAs mentioned above in sera using ELISA.Results: The sensitivity in diagnosing ovarian cancer using single antigen was low, Survivin was the highest of 23.6% and CyclinE was the lowest of 9.1%, but combined with the 6 kinds of TAAs,the sensitivity increased to 63.6%, and specificity was 85.7%, positive and negative predictive proportion,and kappa were 87.5%, 60.0%,and 0.493,respectively.Using the 6 antigens miniarray, positive rate had no difference between different histological types and clinic stages (χ2 was 0.622,0.135,respectively,P>0.05).In primary ovarian cancer patients during chemotherapy after operation, the positive rates (8.7%~34.8%) were tend to decrease.Conclusion: Utilizing the 6 TAAs miniarray to detect autoantibodies in serum may has an important value in diagnosis and state monitoring after operation of ovarian cancer.

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1963年生,博士,教授,博士研究生导师,研究方向:肿瘤流行病学和肿瘤免疫学
更新日期/Last Update: 2010-05-14