[1]千智斌)△,姬明丽),宋晓荣).尼可刹米对新生大鼠海马神经元钠通道的影响[J].郑州大学学报(医学版),2009,(06):1245-1247.
 QIAN Zhibin),JI Mingli),SONG Xiaorong).Effects of nikethamide on sodium channel of hippocampal neurons in neonatal rat[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):1245-1247.
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尼可刹米对新生大鼠海马神经元钠通道的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2009年06期
页码:
1245-1247
栏目:
论著
出版日期:
2009-11-01

文章信息/Info

Title:
Effects of nikethamide on sodium channel of hippocampal neurons in neonatal rat
作者:
千智斌1)姬明丽2)宋晓荣2)
1)新乡医学院机能学实验室 新乡 4530032)新乡医学院病理生理学教研室 新乡 453003
Author(s):
QIAN Zhibin1)JI Mingli2)SONG Xiaorong2)
1)Functional Laboratory,Xinxiang Medical University, Xinxiang 4530032)Department of Pathophysiology,Xinxiang Medical University, Xinxiang 453003
关键词:
尼可刹米海马神经元全细胞膜片钳钠通道大鼠
Keywords:
nikethamidehippocampal neuronwholecell patchclampsodium channelrat
分类号:
R338.2
摘要:
观察尼可刹米对新生大鼠海马神经元电压依赖性钠通道的影响。方法:制备大鼠海马脑组织切片,灌流含5 mg/L尼可刹米的人工脑脊液,应用全细胞膜片钳技术记录给药前、给药后10 min脑组织切片海马神经元电压依赖性钠电流,绘制给药前后钠通道的钠电流测试电压(IV)曲线、稳态激活曲线和稳态失活曲线。结果:尼可刹米在脑电位-50~+20 mV范围内增大钠电流。尼可刹米使钠通道稳态激活曲线中钠通道半数激活电压由(-34.6±3.07) mV变为(-37.63±3.37) mV(t=3.38,P=0.02),曲线向超极化方向变化;尼可刹米使钠通道的稳态失活曲线中钠通道半数失活电压由(-43.36±3.06) mV变为(-33.24±2.05) mV(t=6.13,P=0.002),曲线向去极化方向变化。结论:增加电压依赖性钠电流可能是尼可刹米兴奋中枢神经元的作用机制之一。
Abstract:
To explore the effects of nikethamide on sodium channel of hippocampal neurons in neonatal rat.Methods:The sodium currents in hippocampal neurons of brainstem slice in neonatal rat were recorded using the wholecell patchclamp technique.Results:The sodium current in hippocampal neurons was increased by nikethamide when electrocortical potential changed from -50 to +20 mV. After nikethamide application, the voltage for halfactivation changed from (-34.6±3.07) mV to (-37.63±3.37) mV(t=3.38,P=0.02), the curve of steadystate activation of sodium channel was shifted towards more hyperpolarized potential.After nikethamide application, the voltage for halfinactivation changed from (-43.36±3.06) mV to (-33.24±2.05) mV(t=6.13,P=0.002), the curve of steadystate inactivation of sodium channel was shifted towards positive potential.Conclusion: Increasing the voltagedependent sodium currents might be one reason that nikethamide increases the excitability of neurons.

参考文献/References:

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备注/Memo

备注/Memo:
△男,1974年生,博士,副教授,研究方向:神经电生理
更新日期/Last Update: 2010-05-14