[1]刘桂举,梅家转#,栗敏,等.细胞因子诱导的杀伤细胞免疫表型及其对EC9706细胞杀伤活性的影响*[J].郑州大学学报(医学版),2010,(01):24-26.
 LIU Guiju,MEI Jiazhuan,LI Min,et al.Immunophenotype of cytokineinduced killer cells affect cytotoxic activity against EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):24-26.
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细胞因子诱导的杀伤细胞免疫表型及其对EC9706细胞杀伤活性的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年01期
页码:
24-26
栏目:
食管癌研究
出版日期:
2010-01-30

文章信息/Info

Title:
Immunophenotype of cytokineinduced killer cells affect cytotoxic activity against EC9706 cells
作者:
刘桂举梅家转#栗敏林宏伟李瑞君
郑州人民医院肿瘤内科郑州450003
Author(s):
LIU GuijuMEI JiazhuanLI MinLIN HongweiLI Ruijun
Department of Oncology, Zhengzhou People’s Hospital, Zhengzhou 450003
关键词:
细胞因子诱导的杀伤细胞NKG2D食管癌细胞治疗EC9706细胞
Keywords:
cytokineinduced killer cellNKG2Desophagus carcinomacell therapyEC9706 cell
分类号:
R392.12
文献标志码:
A
摘要:
探讨体外培养细胞因子诱导的杀伤细胞(CIK)过程中CD3+CD56+、NKG2D的动态变化及其与CIK细胞对EC9706杀伤活性的关系。方法:流式细胞仪检测EC9706细胞NKG2D配体的表达;体外分离健康供者外周血单个核细胞,干扰素γ、白细胞介素2、CD3单克隆抗体诱导培养,流式细胞仪检测0、7及14d的细胞CD3+CD56+、NKG2D的表达,乳酸脱氢酶释放法测定0、7及14d的细胞在效靶比201和301时对EC9706细胞的杀伤活性。结果:EC9706细胞表达MICA和ULBP2,不表达MICB、ULBP1和ULBP3。0、7及14d细胞表面NKG2D表达率分别为(26.3±1.1)%、(38.3±0.4)%和(67.1±1.3)%,差异有统计学意义(F=1393.352,P<0.001);CD3+CD56+细胞分别为(0.7±0.1)%、(12.2±1.4)%和(56.5±2.0)%,差异有统计学意义(F=1301.226,P<0.001);0、7及14d细胞对EC9706细胞的杀伤活性:效靶比201时分别为(7.1±2.0)%、(17.4±1.4)%和(37.7±0.2)%,差异有统计学意义(F=354.250,P<0.001);效靶比301时分别为(8.7±0.8)%、(27.2±1.6)%和(44.3±1.1)%,差异有统计学意义(F=648.070,P<0.001)。相关分析表明CIK细胞的杀伤活性与细胞表面NKG2D表达及CIK细胞中CD3+CD56+细胞数有关(r分别为0.992、0.965、0.939和0.929,P<0.001)。结论:体外CIK细胞培养增殖过程中,NKG2D分子表达逐渐上调,CD3+CD56+细胞逐渐增多,CIK细胞对EC9706细胞的杀伤活性逐渐增强。
Abstract:
To investigate the expression of NKG2D ligands in human esophagus carcinoma cell line EC9706, the dynamic changes of CD3+CD56+, NKG2D by cytokineinduced killer (CIK) cells and the relationship with the cytotoxicity of CIK cells against EC9706 cells at different times.Methods:The expression of NKG2D ligands of EC9706 cells were analyzed by flow cytometry. CIK cells were generated from peripheral blood mononuclear cells of healthy donors by culturing cells in the presence of IFNγ, antiCD3 antibody and IL2 in vitro.The percentage of CD3+CD56+ cells and the expression of NKG2D of the CIK cells at days 0, 7, 14 were determined by flow cytometry. Cytotoxicitiy of CIK cells against EC9706 cells were detected by LDH releasing assay at effecttotarget cell ratios of 201,301.Results: It was found that MICA, ULBP2 were expressed, MICB, ULBP1, ULBP3 were not expressed in EC9706 cells. At days 0, 7, 14, the percentage of CD3+CD56+ cells were (0.7±0.1)%, (12.2±1.4)%, (56.5±2.0)%, respectively (F=1 301.226,P<0.001), while the expression of NKG2D by the expanded bulk CIK cells were (26.3±1.1)%, (38.3±0.4)%, (67.1±1.3)%, respectively (F=1 393.352,P<0.001). Cytotoxic activity of CIK cells against EC9706 cells were (7.1±2.0)%, (17.4±1.4)%, (37.7±0.2)% at 201 ET ratios, respectively(F=354.250, P<0.001),(8.7±0.8)%, (27.2±1.6)%, (44.3±1.1)% at 301 ET ratios,respectively (F=648.070, P<0.001). The activity of CIK cells against EC9706 cells was correlated with the NKG2D and CD3+CD56+ expressions(r was 0.992, 0.965, 0.939, and 0.929,P<0.001).Conclusion: During CIK cells expansion, NKG2D and CD3+CD56+ are upregulated,cytotoxic activity of CIK cells increase gradually.

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备注/Memo

备注/Memo:
*郑州市科技计划攻关基金资助项目083SGYS332381;郑州市博士创业基金资助项目郑卫2009175 #通讯作者,男,1966年生,博士,主任医师,研究方向:肿瘤生物治疗,Email:mjzhuan@163.com
更新日期/Last Update: 2010-04-26