[1]黄作平)),何滢),周新华),等.CD99基因转染对霍奇金淋巴瘤L428细胞株表型、细胞周期与凋亡的影响*[J].郑州大学学报(医学版),2010,(01):30-32.
 HUANG Zuoping),HE Ying),ZHOU Xinhua),et al.Effects of CD99 gene transfection on the phenotype,cell cycle distribution and apoptosis of L428 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):30-32.
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CD99基因转染对霍奇金淋巴瘤L428细胞株表型、细胞周期与凋亡的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年01期
页码:
30-32
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
Effects of CD99 gene transfection on the phenotype,cell cycle distribution and apoptosis of L428 cells
作者:
黄作平1)2)何滢2)周新华2)韩西群1)2)张艳1)温宗华1)赵彤1)2)#
1)南方医科大学南方医院病理科广州5105152)广东省分子肿瘤病理学重点实验室广州510515
Author(s):
HUANG Zuoping12)HE Ying2)ZHOU Xinhua2)HAN Xiqun12)ZHANG Yan1) WEN Zonghua1) ZHAO Tong12)
1)Department of Pathology, Nanfang Hospital, Southern Medical University,Guangzhou 5105152)Key Laboratory of Molecular Tumor Pathology of Guangdong Province,Guangzhou 510515
关键词:
CD99增殖凋亡L428细胞株霍奇金淋巴瘤
Keywords:
CD99proliferationapoptosisL428 cells lineHodgkin lymphoma
分类号:
R733.4
文献标志码:
A
摘要:
观察CD99基因转染对霍奇金淋巴瘤L428细胞株表型、细胞周期与凋亡的影响。方法:L428细胞分为3组:转染CD99基因组、转染空质粒组及空白对照组。采用免疫细胞化学方法检测3组细胞CD15、CD30与CD99的表达,并用流式细胞术分别检测细胞周期和细胞凋亡。结果:与其他2组相比,转染CD99基因组L428细胞CD15、CD30表达消失,但可检测到CD99的表达。3组细胞周期分布存在差异,转染CD99基因组细胞阻滞于G2/M期(F=24.85,P<0.001)。3组细胞凋亡率相比,差异有统计学意义(F=45.30,P<0.001),其中转染CD99基因组细胞凋亡率为(12.7±4.6)%,高于转染空质粒组的(4.0±0.9)%和空白对照组的(3.6±1.6)%(P<0.05)。结论:CD99基因可能在霍奇金淋巴瘤的发生中起重要作用。
Abstract:
To observe the effects of CD99 gene transfection on the phenotype,cell cycle distribution and apoptosis of L428 cells.Methods:L428 cells were allocated into 3 groups:cells transfected with CD99 gene, those with empty plasmid,and L428 cells without treatment.Immunocytochemistry was applied to detect CD15, CD30, and CD99 expression.Cell cycle analysis and apoptosis were determined by flow cytometry.Results:Compared with the other 2 groups, L428 cells transfected with CD99 showed no expression of CD15 or CD30, but that of CD99. In addition,cell cycle arrested at G2/M(F=24.85,P<0.001)and increase of cellular apoptosis was detected in L428 cells transfected with CD99(F=45.30,P<0.001).Conclusion: CD99 may play an important role in the occurrence of Hodgkin lymphoma.

参考文献/References:

[1]Lee IS,Kim SH,Song HG,et al.The molecular basis for the generation of Hodgkin and ReedSternberg cells in Hodgkin’s lymphoma[J].Int J Hematol,2003,77(4):330
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[4]李先茂,李燕,赵彤,等. 霍奇金淋巴瘤CD99 基因表达缺失的意义[J].第四军医大学学报,2004,25(23):2 136
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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30770908 #通讯作者,女,1952年生,硕士,教授,研究方向:淋巴瘤的诊断,Email:tongzhao@fimmu.com
更新日期/Last Update: 2010-04-26