[1]马晓君,秦贵军#,闫晓洁,等.高脂肥胖大鼠肝脏组织中叉头状转录因子O1的表达及其与糖脂代谢的关系*[J].郑州大学学报(医学版),2010,(01):44-46.
 MA Xiaojun,QIN Guijun,YAN Xiaojie,et al.Relationship between FoxO1 expression in liver and glycolipid metabolism in highfat diet obese rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):44-46.
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高脂肥胖大鼠肝脏组织中叉头状转录因子O1的表达及其与糖脂代谢的关系*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年01期
页码:
44-46
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
Relationship between FoxO1 expression in liver and glycolipid metabolism in highfat diet obese rats
作者:
马晓君秦贵军#闫晓洁樊大贝
郑州大学第一附属医院内分泌科郑州450052
Author(s):
MA Xiaojun QIN Guijun YAN Xiaojie FAN Dabei
Department of Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
叉头状转录因子O1肥胖高脂饮食大鼠
Keywords:
FoxO1obesityhighfat dietrat
分类号:
R587.1
文献标志码:
A
摘要:
观察高脂肥胖大鼠肝脏组织中叉头状因子O1(FoxO1)的表达,探讨其与血糖、血脂代谢的关系。方法:雄性SD大鼠30只,随机分为对照组(10只,给予常规饲料)和高脂组(20只,给予高脂饲料),共饲养10周。饲养期末取符合肥胖标准的16只大鼠定为肥胖组。检测2组大鼠空腹血糖(FBG)、胰岛素(Ins)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇指数(HDLC)和低密度脂蛋白胆固醇(LDLC)水平;采用稳态模型指数(HOMAIR)评价胰岛素抵抗程度;实时荧光定量RTPCR检测2组大鼠肝脏FoxO1mRNA的表达。结果:饲养10周后,肥胖组大鼠FBG、Ins、TG、TC、LDLC和HOMAIR水平均较对照组升高(t分别为1.952、25.566、8.642、5.845、9.922和23.234,P均<0.05)。与对照组的(19.3±5.1)相比,肥胖组大鼠肝脏FoxO1mRNA表达量(35.6±4.4)增加(t=8.649,P<0.001)。肥胖组大鼠肝脏FoxO1mRNA表达与FBG(r=0.565,P=0.004)、Ins(r=0.564,P=0.004)、TG(r=0.626,P=0.001)和HOMAIR(r=0.578,P=0.003)呈正相关。结论:高脂肥胖大鼠肝脏组织中FoxO1的表达量升高,且其与血糖、血脂代谢有关,可能参与肥胖和胰岛素抵抗的发病。
Abstract:
To explore the association of the expression of FoxO1 in liver with glycolipid metabolism in highfat diet obese rats.Methods:Thirty male SD rats were randomly divided into 2 groups by taking routine (n=10) and highfat diet (n=20). Levels of fasting blood glucose (FBG), serum insulin (Ins), triglyceride (TG), total cholesterol (TC), high density lipoproteinC(HDLC) and low density lipoproteinC(LDLC) were measured 10 weeks later. Insulin resistance was assessed by HOMAIR. The FoxO1 mRNA expression level in liver was detected by realtime quantitative RTPCR.Results: Levels of FBG, Ins, TG, TC, LDLC and HOMAIR were significantly higher in obese group than those in control group 10 weeks later(t=1.952,25.566,8.642,5.845,9.922,23.234,P<0.05). The mRNA level of FoxO1 in liver of obese group (35.6±4.4) was higher compared with control group(19.3±5.1) (t=8.649,P<0.001).The expression level of FoxO1 mRNA in liver was positively correlated with FBG(r=0.565,P=0.004), Ins(r=0.564,P=0.004), TG(r=0.626,P=0.001) and HOMAIR(r=0.578,P=0.003).Conclusion: The increased expression of FoxO1 in liver of highfat diet obese rats is associated with blood glucose and lipid levels. FoxO1 may be related to obesity and insulin resistance.

参考文献/References:

[1]Accili D, Arden KC. FoxOs at the crossroads of cellular metabolism, differentiation, and transformation[J]. Cell, 2004, 117(4):421
[2]Kaestner KH, Knochel W, Martinez DE. Unified nomenclature for the winged helix/forkhead transcription factors[J].Genes Dev, 2000, 14(2):142
[3]Farmer SR. The forkhead transcription factor Foxo1: a possible link between obesity and insulin resistance[J]. Mol Cell, 2003, 11(1): 6
[4]Zhang W, Patil S, Chauhan B, et al. Foxo1 regulates multiple metabolic pathwas in the liver: effects on gluconeogenic, glycolytic, and lipogenic gene expression[J]. J Biol Chem,2006,281(15):10 105
[5]张德利,刘正娟,李永昕,等. 高脂喂养大鼠血清瘦素水平与肝脏瘦素受体mRNA的表达[J]. 中华内分泌代谢杂志,2005,21(3):273
[6]钱伯初,史红,吕燕萍. 肥胖动物模型的研究进展[J].中国新药杂志,2007,16(15):1 159
[7]朱永生,刘峰,郭锡镕. 脂联素受体2基因在饮食诱导肥胖大鼠肝脏组织中的表达[J].实用儿科临床杂志,2007,22(19):1 479
[8]Barthel A, Schmoll D, Unterman TG. FoxO proteins in insulin action and metabolism[J].Trends Endocrinol Metab, 2005,16(4):183
[9]Qu S, Altomonte J, Perdomo G, et al. Aberrant FoxO1 function is associated with impaired hepatic metabolism[J]. Endocrinology, 2006, 147(12):5 641
[10]Altomonte J, Cong L, Harbaran S, et al. Foxo1 mediates insulin action on apoCⅢ and triglyceride metabolism[J].J Clin Invest, 2004, 114(10):1 493
[11]张庆华,吕国才. 高甘油三酯患者血清载脂蛋白谱分析[J]. 浙江医学,2007,29(10):1 023

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备注/Memo

备注/Memo:
*河南省创新型科技人才队伍建设工程基金资助项目84100410016 #通讯作者,男,1965年生,博士,教授,主任医师,研究方向:糖尿病的发病机制及防治,Email:hyqingj@zzu.edu.cn
更新日期/Last Update: 2010-04-26