[1]高铁杰,李卫民,刘毅,等.北京昌平区北京W系结核分枝杆菌的流行特征*[J].郑州大学学报(医学版),2010,(01):47-50.
 GAO Tiejie,LI Weimin,LIU Yi,et al.Characteristics of epidemiology of Beijing/W lineage Mycobacterium tuberculosis isolates in Changping, Beijing[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):47-50.
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北京昌平区北京W系结核分枝杆菌的流行特征*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年01期
页码:
47-50
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
Characteristics of epidemiology of Beijing/W lineage Mycobacterium tuberculosis isolates in Changping, Beijing
作者:
高铁杰1李卫民2刘毅2张治国1周辉1
1)北京市昌平区结核病防治所北京1022002)北京市结核病胸部肿瘤研究所北京1011493)中国动物卫生与流行病学中心青岛266032
Author(s):
GAO Tiejie1 LI Weimin2LIU Yi2ZHANG Zhiguo2ZHOU Hui1QIU Yunqing2TIAN Miao2ZHANG Xuxia2 ZHANG Jianyuan2 HAN Yuefei1GAO Feng3LI Chuanyou2
1)Changping District Tuberculosis Control Institute, Beijing 1022002)Beijing Tuberculosis and Thoratic Tumor Research Institute, Beijing 1011493)China Animal Health and Epidemiology Center, Qingdao 266032
关键词:
结核分枝杆菌北京/W系进化树北京流行病学
分类号:
R181.3+3
文献标志码:
A
摘要:
探讨北京/W系结核分枝杆菌在北京市昌平区的流行特征。方法:连续收集北京市昌平区2004年1月1日至2006年12月31日的结核分枝杆菌分离株336株,采用Spoligotyping和多重PCR法对其进行分型及亚分型;同时采用谱系定义靶标RD105、RD181、RD150、RD142和RealtimePCR方法进行分型和亚分型,并分析北京/W系的2个亚系与其对应患者年龄、出生地、抗结核药物敏感谱和卡介苗(BCG)接种情况之间的关系。结果:2种方法对该地区结核分枝杆菌分型及亚分型结果完全一致,均为:299株为北京/W系和37株为非北京/W系结核分枝杆菌;在北京/W系结核分枝杆菌中,存在RD181的非典型北京菌株47株(15.7%),而相对现代的缺失RD181的W菌/典型北京家族菌株252株(84.3%);在W菌/典型北京家族菌株中,RD150和RD142缺失的菌株分别为168和173株。W菌/典型北京家族菌株和非典型北京菌株2个亚系的分布与患者出生地、BCG接种情况、年龄以及抗结核药物敏感谱(链霉素、异烟肼、利福平和乙胺丁醇)均无关(χ2分别为0.530、1.623、2.180、<0.001、0.849、0.038和0.006,P均>0.05)。结论:初步绘制出北京市昌平区结核分枝杆菌的系统进化树,其中北京/W系呈现较为明显的优势。北京/W系中较现代的W菌/典型北京家族菌株又占明显优势。以RD105为靶标的RealtimePCR方法鉴定北京/W系结核分枝杆菌快速、准确。

参考文献/References:

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[4]李卫民,王苏民, 裴秀英,等. 北京、广东、宁夏三地结核分支杆菌DNA指纹的应用研究[J].中华流行病学杂志,2003,24(5):381
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备注/Memo

备注/Memo:
*北京市自然科学基金资助项目5062016;北京市优秀人才培养专项基金资助项目 #通讯作者,男,1966年生,博士,研究员,研究方向:结核病细菌免疫学,Email:lichuanyou6688@hotmail.com
更新日期/Last Update: 2010-04-26