[1]李丹),杨冉),曾华金),等.RPHPLC同时测定金钱通淋颗粒中槲皮素、木犀草素及山奈酚的含量[J].郑州大学学报(医学版),2010,(01):145-146.
 LI Dan),YANG Ran),ZENG Huajin),et al.RPHPLC determination of contents of quercetin,luteolin and kaeampferol in Jinqiantonglin granule[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):145-146.
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RPHPLC同时测定金钱通淋颗粒中槲皮素、木犀草素及山奈酚的含量()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年01期
页码:
145-146
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
RPHPLC determination of contents of quercetin,luteolin and kaeampferol in Jinqiantonglin granule
作者:
李丹1)杨冉1)曾华金2)屈凌波13)#
1)郑州大学化学系郑州4500012)郑州大学药学院郑州4500013)河南工业大学郑州450001
Author(s):
LI Dan1)YANG Ran1)ZENG Huajin2)QU Lingbo13)
1)Department of Chemistry,Zhengzhou University, Zhengzhou 4500012)School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 4500013)Henan University of Technology, Zhengzhou 450001
关键词:
槲皮素木犀草素山奈酚金钱通淋颗粒
Keywords:
quercetin luteolin kaeampferol Jinqiantonglin granule
分类号:
R969
文献标志码:
A
摘要:
建立同时测定金钱通淋颗粒中主要有效成分槲皮素、木犀草素及山奈酚的高效液相色谱(HPLC)方法体系。方法:色谱条件:色谱柱KromasilC18(4.6mm×250mm,5μm),柱温34℃,流动相V(甲醇)V(体积分数0.4%磷酸)=5545,流速1.0mL/min,检测波长360nm,以外标法定量。结果:槲皮素、木犀草素和山奈酚分别在(0.25~25.00),(0.14~11.00)和(0.26~33.00)mg/L范围内线性关系良好。3者的平均回收率分别为99.6%、100.3%和99.8%,相应的RSD分别为0.99%、0.47%和0.67%。供试品溶液在24h内稳定。结论:该方法准确、灵敏,对进一步完善金钱通淋颗粒的内在质量控制标准具有重要的参考价值。
Abstract:
To establish a RPHPLC method for determining quercetin, luteolin and kaeampferol synchronously of Jinqiantonglin granule.Methods:The sample was separated on a Kromasil C18 column at the temperature of 34 ℃. The mobile phase was a mixture of methanol and 0.4% phosphoric acid water solution(5545 in volume) with the flowrate of 1.0 mL/min and the detection wavelength of 360 nm.Results: The calibration curves of three flavonoids were linear in the range of (0.25~25.00),(0.14~11.00) and (0.26~33.00) mg/L,respectively. Their average recoveries were 99.6%,100.3% and 99.8% with corresponding RSD of 0.97%,0.47% and 0.67%,respectively.The sample solution was stable within 24 hours.Conclusion: The method is accurate with high sensitivity and is of great value to control the quality of Jinqiantonglin granule.

参考文献/References:

[1]温梅云,钟球,钟鸣. HPLC法测定通淋颗粒中槲皮素和山奈素的含量[J].国际医药卫生导报,2007,13(4):86
[2]曹萍,褚小兰,范崔生.金钱草类中药的研究概况[J].江西医学院学报,2005,45(1):110
[3]钱建明.海金沙的质量监控[J].浙江中西医结合杂志,2005,15(5):323
[4]国家药典委员会.中国药典[M].Ⅰ部.北京:化学工业出版社,2005.

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备注/Memo

备注/Memo:
#通讯作者,男,1963年生,教授,研究方向:分析化学,Email:qulingbo@zzu.edu.cn
更新日期/Last Update: 2010-04-26